Nitric oxide synthesis is increased in cybrid cells with m.3243A>G mutation

Juliana Gamba, Luana T. Gamba, Gabriela S. Rodrigues, Beatriz H. Kiyomoto, Carlos T Moraes, Celia H. Tengan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Nitric oxide (NO) is a free radical and a signaling molecule in several pathways, produced by nitric oxide synthase (NOS) from the conversion of L-arginine to citrulline. Supplementation of L-arginine has been used to treat MELAS (mitochondrial encephalopathy with lactic acidosis and stroke like syndrome), a mitochondrial disease caused by the m.3243A>G mutation. Low levels of serum arginine and endothelium dysfunction have been reported in MELAS and this treatment may increase NO in endothelial cells and promote vasodilation, decreasing cerebral ischemia and strokes. Although clinical benefits have been reported, little is known about NO synthesis in MELAS. In this study we found that osteosarcoma derived cybrid cells with high levels of m.3243A>G had increased nitrite, an NO metabolite, and increased intracellular NO, demonstrated by an NO fluorescent probe (DAF-FM). Muscle vessels from patients with the same mutation had increased staining in NADPH diaphorase, suggestive of increased NOS. These results indicate increased production of NO in cells harboring the m.3243A>G, however no nitrated protein was detected by Western blotting. Further studies are necessary to clarify the exact mechanisms of L-arginine effect to determine the appropriate clinical use of this drug therapy.

Original languageEnglish
Pages (from-to)394-410
Number of pages17
JournalInternational Journal of Molecular Sciences
Volume14
Issue number1
DOIs
StatePublished - Jan 1 2013

Fingerprint

Nitric oxide
nitric oxide
mutations
Nitric Oxide
MELAS Syndrome
Mutation
Arginine
synthesis
cells
Nitric Oxide Synthase
strokes
Stroke
Drug therapy
NADPH Dehydrogenase
acidosis
vasodilation
Mitochondrial Diseases
Citrulline
Lactic Acidosis
Endothelial cells

Keywords

  • Arginine
  • Mitochondria
  • Mitochondrial disease
  • Mitochondrial DNA
  • Nitric oxide

ASJC Scopus subject areas

  • Computer Science Applications
  • Molecular Biology
  • Catalysis
  • Inorganic Chemistry
  • Spectroscopy
  • Organic Chemistry
  • Physical and Theoretical Chemistry

Cite this

Nitric oxide synthesis is increased in cybrid cells with m.3243A>G mutation. / Gamba, Juliana; Gamba, Luana T.; Rodrigues, Gabriela S.; Kiyomoto, Beatriz H.; Moraes, Carlos T; Tengan, Celia H.

In: International Journal of Molecular Sciences, Vol. 14, No. 1, 01.01.2013, p. 394-410.

Research output: Contribution to journalArticle

Gamba, Juliana ; Gamba, Luana T. ; Rodrigues, Gabriela S. ; Kiyomoto, Beatriz H. ; Moraes, Carlos T ; Tengan, Celia H. / Nitric oxide synthesis is increased in cybrid cells with m.3243A>G mutation. In: International Journal of Molecular Sciences. 2013 ; Vol. 14, No. 1. pp. 394-410.
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