Nicotine upregulates nerve growth factor expression and prevents apoptosis of cultured spinal cord neurons

Rosario Garrido, Kelley King-Pospisil, Kwang Won Son, Bernhard Hennig, Michal J Toborek

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Modulation of neurotrophic factor expression may constitute an important part of neuroprotective effects of nicotine. Therefore, the effects of nicotine on expression of nerve growth factor (NGF) and its receptor, tyrosine receptor kinase A (trkA), were studied in cultured spinal cord neurons treated with arachidonic acid. Because injury to spinal cord is associated with elevated levels of arachidonic acid, this cell culture system has been developed in our laboratory as an in vitro model of neuronal injury in spinal cord trauma. Treatment with nicotine markedly upregulated NGF mRNA and protein expression in spinal cord neurons. In addition, a 12h treatment with nicotine increased mRNA levels of trkA. Both nicotine and exogenous NGF inhibited arachidonic acid induced apoptosis of spinal cord neurons. However, the blockage of the trkA receptor prevented nicotine-mediated anti-apoptotic effects. The present results indicate that increased expression of NGF may be an important element of the neuroprotective effects of nicotine in injured spinal cord neurons.

Original languageEnglish
Pages (from-to)349-355
Number of pages7
JournalNeuroscience Research
Volume47
Issue number3
DOIs
StatePublished - Nov 1 2003
Externally publishedYes

Fingerprint

Nerve Growth Factor
Nicotine
Spinal Cord
Up-Regulation
Apoptosis
Neurons
Receptor Protein-Tyrosine Kinases
Arachidonic Acid
Neuroprotective Agents
Spinal Cord Injuries
Nerve Growth Factor Receptor
Messenger RNA
Nerve Growth Factors
Cell Culture Techniques
Wounds and Injuries
Proteins

Keywords

  • Fatty acids
  • Neuroprotection
  • Nicotine
  • Spinal cord neurons
  • Spinal cord trauma

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Nicotine upregulates nerve growth factor expression and prevents apoptosis of cultured spinal cord neurons. / Garrido, Rosario; King-Pospisil, Kelley; Son, Kwang Won; Hennig, Bernhard; Toborek, Michal J.

In: Neuroscience Research, Vol. 47, No. 3, 01.11.2003, p. 349-355.

Research output: Contribution to journalArticle

Garrido, Rosario ; King-Pospisil, Kelley ; Son, Kwang Won ; Hennig, Bernhard ; Toborek, Michal J. / Nicotine upregulates nerve growth factor expression and prevents apoptosis of cultured spinal cord neurons. In: Neuroscience Research. 2003 ; Vol. 47, No. 3. pp. 349-355.
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