Nicotine indirectly inhibits [3H]dopamine uptake at concentrations that do not directly promote [3H]dopamine release in rat striatum

Sari E Izenwasser, Henry M. Jacocks, John G. Rosenberger, Brian M. Cox

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The effects of both (-)- and (+)-nicotine isomers were examined on in vitro uptake and release of [3H]dopamine in rat striatum. Both isomers inhibited uptake of [3H]dopamine in chopped tissue at concentrations well below those necessary for promoting release of preloaded [3H]dopamine. (-)-Nicotine was more potent than (+)-nicotine both at inhibiting uptake and at promoting release. Unlike other dopamine uptake inhibitors, however, nicotine inhibited only 50% of the total uptake. In the presence of 1 nM nicotine, the residual [3H]dopamine uptake was less sensitive to inhibition by cocaine than uptake in the absence of nicotine. Nicotine did not compete against the binding of [3H]GBR 12935, a selective dopamine uptake inhibitor. The nicotinic receptor agonists carbachol and 1,1-dimethyl-4 phenylpiperazinium iodide also inhibited uptake, whereas the nicotinic antagonists chlorisondamine and mecamylamine blocked nicotine's effect. Thus, the effect of nicotine on dopamine uptake appears to be mediated by a receptor similar to the nicotinic acetylcholine receptor. These receptors do not seem to be on the terminals that are accumulating dopamine, however, since tetrodotoxin prevented the effect of nicotine on [3H]dopamine uptake and nicotine had no effect on uptake in a synaptosomal preparation.

Original languageEnglish
Pages (from-to)603-610
Number of pages8
JournalJournal of Neurochemistry
Volume56
Issue number2
StatePublished - Dec 1 1991
Externally publishedYes

Fingerprint

Nicotine
Rats
Dopamine
Dopamine Uptake Inhibitors
Nicotinic Receptors
Isomers
Chlorisondamine
Nicotinic Agonists
Nicotinic Antagonists
Mecamylamine
Tetrodotoxin
Carbachol
Iodides
Cocaine
Tissue

Keywords

  • Cocaine
  • Dopamine release
  • Dopamine uptake
  • Nicotine

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Nicotine indirectly inhibits [3H]dopamine uptake at concentrations that do not directly promote [3H]dopamine release in rat striatum. / Izenwasser, Sari E; Jacocks, Henry M.; Rosenberger, John G.; Cox, Brian M.

In: Journal of Neurochemistry, Vol. 56, No. 2, 01.12.1991, p. 603-610.

Research output: Contribution to journalArticle

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