Nicotine augments glomerular injury in a rat model of acute nephritis

Edgar A. Jaimes, Run Xia Tian, Mahesh S. Joshi, Leopoldo Raij

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background/Aims: Epidemiologic studies suggest that cigarette smoke worsens the progression of renal injury in patients with glomerular diseases. The mechanisms involved have not been elucidated. These studies were designed to determine whether nicotine worsens markers of inflammation including glomerular cell proliferation and fibronectin deposition in an in vivomodel of glomerular injury. Methods: Sprague-Dawley rats were injected with anti-Thy1 antibody and given either tap water or nicotine in the drinking water until sacrifice at day 14. Fibronectin expression was measured by Western blot and immunohistochemistry. COX-2 expression was also determined by Western blot in the kidney cortex of rats treated with nicotine and in cultured human mesangial cells treated with nicotine. Results: Anti-Thy1 antibody administration resulted in a significant increase in the number of cells per glomerulus that was further increased by the administration of nicotine. In nephritic rats, the administration of nicotine significantly increased fibronectin and COX-2 expression. In cultured human mesangial cells we also demonstrated that nicotine increases COX-2 expression and activity and that COX-2 mediates mesangial cell proliferation in response to nicotine. Conclusion: Either in vivo or in vitro treatment with nicotine leads to activation of inflammatory mediators and hallmarks of glomerular injury, which may explain the mechanisms involved in the deleterious effects of cigarette smoking on renal disease.

Original languageEnglish
Pages (from-to)319-326
Number of pages8
JournalAmerican Journal of Nephrology
Volume29
Issue number4
DOIs
StatePublished - Mar 1 2009

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Nephritis
Nicotine
Wounds and Injuries
Mesangial Cells
Fibronectins
Kidney
Anti-Idiotypic Antibodies
Western Blotting
Cell Proliferation
Kidney Cortex
Smoke
Tobacco Products
Drinking Water
Sprague Dawley Rats
Epidemiologic Studies
Cell Count
Smoking
Immunohistochemistry
Inflammation
Water

Keywords

  • Cigarette smoking
  • Glomerulonephritis
  • Kidney glomerulus
  • Nicotine

ASJC Scopus subject areas

  • Nephrology

Cite this

Nicotine augments glomerular injury in a rat model of acute nephritis. / Jaimes, Edgar A.; Tian, Run Xia; Joshi, Mahesh S.; Raij, Leopoldo.

In: American Journal of Nephrology, Vol. 29, No. 4, 01.03.2009, p. 319-326.

Research output: Contribution to journalArticle

Jaimes, Edgar A. ; Tian, Run Xia ; Joshi, Mahesh S. ; Raij, Leopoldo. / Nicotine augments glomerular injury in a rat model of acute nephritis. In: American Journal of Nephrology. 2009 ; Vol. 29, No. 4. pp. 319-326.
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