Nicotine attenuates arachidonic acid-induced neurotoxicity in cultured spinal cord neurons

Rosario Garrido, Andrzej Malecki, Bernhard Hennig, Michal J Toborek

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Arachidonic acid release from cellular membranes due to spinal cord trauma may be one of the principal destructive events that can lead to progressive injury to spinal cord tissue. Exposure to arachidonic acid can compromise neuronal survival and viability. Because nicotine is known to be a neuroprotective agent, we propose that it can prevent arachidonic acid- induced neurotoxicity. To study this hypothesis, effects of nicotine on mitochondrial function, cellular energy content and apoptotic cell death were measured in cultured spinal cord neurons treated with arachidonic acid. Nicotine attenuated arachidonic acid-induced compromised cell viability and cellular ATP levels in spinal cord neurons. Nicotine exerted these protective effects when used at the concentration of 10 μM and only after a 2-h pre- treatment before a co-exposure to arachidonic acid. Antagonists of nicotinic receptors, such as α-bungarotoxin or mecamylamine, only partially reversed these neuroprotective effects of nicotine. In addition, nicotine prevented arachidonic acid-induced activation of caspase-3 activity and apoptotic cell death. These results indicate that nicotine pre-treatment can exert a protective effect against arachidonic acid-induced injury to spinal cord neurons. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)59-68
Number of pages10
JournalBrain Research
Volume861
Issue number1
DOIs
StatePublished - Apr 7 2000
Externally publishedYes

Fingerprint

Nicotine
Arachidonic Acid
Spinal Cord
Neurons
Spinal Cord Injuries
Neuroprotective Agents
Cell Death
Mecamylamine
Bungarotoxins
Nicotinic Receptors
Caspase 3
Cell Survival
Adenosine Triphosphate
Membranes

Keywords

  • Apoptosis
  • Free fatty acid
  • Nicotinic receptor
  • Spinal cord trauma

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Nicotine attenuates arachidonic acid-induced neurotoxicity in cultured spinal cord neurons. / Garrido, Rosario; Malecki, Andrzej; Hennig, Bernhard; Toborek, Michal J.

In: Brain Research, Vol. 861, No. 1, 07.04.2000, p. 59-68.

Research output: Contribution to journalArticle

Garrido, Rosario ; Malecki, Andrzej ; Hennig, Bernhard ; Toborek, Michal J. / Nicotine attenuates arachidonic acid-induced neurotoxicity in cultured spinal cord neurons. In: Brain Research. 2000 ; Vol. 861, No. 1. pp. 59-68.
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