Nicotinamide-rich diet in dba/2j mice preserves retinal ganglion cell metabolic function as assessed by perg adaptation to flicker

Tsung Han Chou, Giovanni Luca Romano, Rosario Amato, Vittorio Porciatti

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Flickering light increases metabolic demand in the inner retina. Flicker may exacerbate defective mitochondrial function in glaucoma, which will be reflected in the pattern electroretinogram (PERG), a sensitive test of retinal ganglion cell (RGC) function. We tested whether flicker altered the PERG of DBA/2J (D2) glaucomatous mice and whether vitamin B3-rich diet contributed to the flicker effect. D2 mice fed with either standard chow (control, n = 10) or chow/water enriched with nicotinamide (NAM, 2000 mg/kg per day) (treated, n = 10) were monitored from 3 to 12 months. The PERG was recorded with superimposed flicker (F-PERG) at either 101 Hz (baseline) or 11 Hz (test), and baseline-test amplitude difference (adaptation) evaluated. At endpoint, flat-mounted retinas were immunostained (RBPMS and mito-tracker). F-PERG adaptation was 41% in 3-month-old D2 and decreased with age more in control D2 than in NAM-fed D2 (GEE, p < 0.01). At the endpoint, F-PERG adaptation was 0% in control D2 and 17.5% in NAM-fed D2, together with higher RGC density (2.4×), larger RGC soma size (2×), and greater intensity of mitochondrial staining (3.75×). F-PERG adaptation may provide a non-invasive tool to assess RGC autoregulation in response to increased metabolic demand and test the effect of dietary/pharmacological treatments on optic nerve disorders.

Original languageEnglish (US)
Article number1910
JournalNutrients
Volume12
Issue number7
DOIs
StatePublished - Jul 2020

Keywords

  • Adaptation
  • Mitochondria
  • Nicotinamide
  • Pattern electroretinogram
  • Retinal ganglion cell

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

Fingerprint Dive into the research topics of 'Nicotinamide-rich diet in dba/2j mice preserves retinal ganglion cell metabolic function as assessed by perg adaptation to flicker'. Together they form a unique fingerprint.

Cite this