Nicotinamide mononucleotide adenylyltransferase maintains active zone structure by stabilizing Bruchpilot

Shaoyun Zang, Yousuf O. Ali, Kai Ruan, R. Grace Zhai

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Active zones are specialized presynaptic structures critical for neurotransmission. We show that a neuronal maintenance factor, nicotinamide mononucleotide adenylyltransferase (NMNAT), is required for maintaining active zone structural integrity in Drosophila by interacting with the active zone protein, Bruchpilot (BRP), and shielding it from activity-induced ubiquitin-proteasome-mediated degradation. NMNAT localizes to the peri-active zone and interacts biochemically with BRP in an activity-dependent manner. Loss of NMNAT results in ubiquitination, mislocalization and aggregation of BRP, and subsequent active zone degeneration. We propose that, as a neuronal maintenance factor, NMNAT specifically maintains active zone structure by direct protein-protein interaction.

Original languageEnglish (US)
Pages (from-to)87-94
Number of pages8
JournalEMBO Reports
Volume14
Issue number1
DOIs
StatePublished - Jan 2013

Keywords

  • active zone
  • BRP
  • chaperone
  • NMNAT
  • protein homeostasis

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biochemistry

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