NGF, BDNF and NT-3 play unique roles in the in vitro development and patterning of innervation of the mammalian inner ear

Hinrich Staecker, Thomas R. Van De Water, Philippe P. Lefebvre, Wei Liu, Masseih Moghadassi, Vera Galinovic-Schwartz, Brigitte Malgrange, Gustave Moonen

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Developing cochleovestibular ganglion (CVG) neurons depend upon interaction with the otocyst, their peripheral target tissue, for both trophic support and tropic guidance. RT-PCR of E11 through E14 otocyst-CVG RNA extracts have shown that NGF as well as BDNF and NT-3 are expressed in the developing inner ear (in situ RT-PCR on tissue sections of E12 otocysts localized all three neurotrophins to the otocyst). To evaluate the functional significance of NGF, BDNF and NT-3 expression, E10.5 otocyst-CVG explants were treated with antisense oligonucleotides and compared to sense treated and control cultures. Confocal microscopic analysis revealed that treatment with BDNF antisense resulted in extensive neuronal cell death, downregulation of NGF caused an inhibition of neuritogenesis and a decrease in the neuronal population of the CVG, whereas treatment with NT-3 antisense resulted in a loss of target directed CVG neuritic ingrowth in this in vitro model. The effect of NGF or BDNF antisense treatment could be prevented by the simultaneous addition of the respective growth factor. These findings demonstrate that each of the three neurotrophins have important roles during the onset of neuritic ingrowth of the CVG neurons to the otocyst.

Original languageEnglish (US)
Pages (from-to)49-60
Number of pages12
JournalDevelopmental Brain Research
Volume92
Issue number1
DOIs
StatePublished - Mar 29 1996

Keywords

  • Antisense oligonucleotide
  • Cochleovestibular ganglion
  • In vitro
  • Neurite guidance
  • Neuritogenesis
  • Neuronal survival
  • Neurotrophin
  • Otocyst

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

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    Staecker, H., Van De Water, T. R., Lefebvre, P. P., Liu, W., Moghadassi, M., Galinovic-Schwartz, V., Malgrange, B., & Moonen, G. (1996). NGF, BDNF and NT-3 play unique roles in the in vitro development and patterning of innervation of the mammalian inner ear. Developmental Brain Research, 92(1), 49-60. https://doi.org/10.1016/0165-3806(95)00198-0