Next generation sequencing: Implications in personalized medicine and pharmacogenomics

Bahareh Rabbani, Hirofumi Nakaoka, Shahin Akhondzadeh, Mustafa Tekin, Nejat Mahdieh

Research output: Contribution to journalReview article

33 Citations (Scopus)

Abstract

A breakthrough in next generation sequencing (NGS) in the last decade provided an unprecedented opportunity to investigate genetic variations in humans and their roles in health and disease. NGS offers regional genomic sequencing such as whole exome sequencing of coding regions of all genes, as well as whole genome sequencing. RNA-seq offers sequencing of the entire transcriptome and ChIP-seq allows for sequencing the epigenetic architecture of the genome. Identifying genetic variations in individuals can be used to predict disease risk, with the potential to halt or retard disease progression. NGS can also be used to predict the response to or adverse effects of drugs or to calculate appropriate drug dosage. Such a personalized medicine also provides the possibility to treat diseases based on the genetic makeup of the patient. Here, we review the basics of NGS technologies and their application in human diseases to foster human healthcare and personalized medicine.

Original languageEnglish (US)
Pages (from-to)1818-1830
Number of pages13
JournalMolecular BioSystems
Volume12
Issue number6
DOIs
StatePublished - 2016

Fingerprint

Precision Medicine
Pharmacogenetics
Genome
Exome
Transcriptome
Epigenomics
Pharmaceutical Preparations
Disease Progression
RNA
Technology
Delivery of Health Care
Health
Genes

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

Cite this

Next generation sequencing : Implications in personalized medicine and pharmacogenomics. / Rabbani, Bahareh; Nakaoka, Hirofumi; Akhondzadeh, Shahin; Tekin, Mustafa; Mahdieh, Nejat.

In: Molecular BioSystems, Vol. 12, No. 6, 2016, p. 1818-1830.

Research output: Contribution to journalReview article

Rabbani, Bahareh ; Nakaoka, Hirofumi ; Akhondzadeh, Shahin ; Tekin, Mustafa ; Mahdieh, Nejat. / Next generation sequencing : Implications in personalized medicine and pharmacogenomics. In: Molecular BioSystems. 2016 ; Vol. 12, No. 6. pp. 1818-1830.
@article{e674e9a426c843b590fb54febdbf894e,
title = "Next generation sequencing: Implications in personalized medicine and pharmacogenomics",
abstract = "A breakthrough in next generation sequencing (NGS) in the last decade provided an unprecedented opportunity to investigate genetic variations in humans and their roles in health and disease. NGS offers regional genomic sequencing such as whole exome sequencing of coding regions of all genes, as well as whole genome sequencing. RNA-seq offers sequencing of the entire transcriptome and ChIP-seq allows for sequencing the epigenetic architecture of the genome. Identifying genetic variations in individuals can be used to predict disease risk, with the potential to halt or retard disease progression. NGS can also be used to predict the response to or adverse effects of drugs or to calculate appropriate drug dosage. Such a personalized medicine also provides the possibility to treat diseases based on the genetic makeup of the patient. Here, we review the basics of NGS technologies and their application in human diseases to foster human healthcare and personalized medicine.",
author = "Bahareh Rabbani and Hirofumi Nakaoka and Shahin Akhondzadeh and Mustafa Tekin and Nejat Mahdieh",
year = "2016",
doi = "10.1039/c6mb00115g",
language = "English (US)",
volume = "12",
pages = "1818--1830",
journal = "Molecular BioSystems",
issn = "1742-206X",
publisher = "Royal Society of Chemistry",
number = "6",

}

TY - JOUR

T1 - Next generation sequencing

T2 - Implications in personalized medicine and pharmacogenomics

AU - Rabbani, Bahareh

AU - Nakaoka, Hirofumi

AU - Akhondzadeh, Shahin

AU - Tekin, Mustafa

AU - Mahdieh, Nejat

PY - 2016

Y1 - 2016

N2 - A breakthrough in next generation sequencing (NGS) in the last decade provided an unprecedented opportunity to investigate genetic variations in humans and their roles in health and disease. NGS offers regional genomic sequencing such as whole exome sequencing of coding regions of all genes, as well as whole genome sequencing. RNA-seq offers sequencing of the entire transcriptome and ChIP-seq allows for sequencing the epigenetic architecture of the genome. Identifying genetic variations in individuals can be used to predict disease risk, with the potential to halt or retard disease progression. NGS can also be used to predict the response to or adverse effects of drugs or to calculate appropriate drug dosage. Such a personalized medicine also provides the possibility to treat diseases based on the genetic makeup of the patient. Here, we review the basics of NGS technologies and their application in human diseases to foster human healthcare and personalized medicine.

AB - A breakthrough in next generation sequencing (NGS) in the last decade provided an unprecedented opportunity to investigate genetic variations in humans and their roles in health and disease. NGS offers regional genomic sequencing such as whole exome sequencing of coding regions of all genes, as well as whole genome sequencing. RNA-seq offers sequencing of the entire transcriptome and ChIP-seq allows for sequencing the epigenetic architecture of the genome. Identifying genetic variations in individuals can be used to predict disease risk, with the potential to halt or retard disease progression. NGS can also be used to predict the response to or adverse effects of drugs or to calculate appropriate drug dosage. Such a personalized medicine also provides the possibility to treat diseases based on the genetic makeup of the patient. Here, we review the basics of NGS technologies and their application in human diseases to foster human healthcare and personalized medicine.

UR - http://www.scopus.com/inward/record.url?scp=84971324208&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84971324208&partnerID=8YFLogxK

U2 - 10.1039/c6mb00115g

DO - 10.1039/c6mb00115g

M3 - Review article

C2 - 27066891

AN - SCOPUS:84971324208

VL - 12

SP - 1818

EP - 1830

JO - Molecular BioSystems

JF - Molecular BioSystems

SN - 1742-206X

IS - 6

ER -