New ubiquitin-positive intraneuronal inclusions in the extra-motor cortices in patients with amyotrophic lateral sclerosis

Koichi Okamoto, Shunsaku Hirai, Tsuneo Yamazaki, Xiaoyan Sun, Yoichi Nakazato

Research output: Contribution to journalArticle

216 Citations (Scopus)

Abstract

We examined the brains of 27 amyotrophic lateral sclerosis (ALS) patients and 50 controls by light, electron and immunoelectron microscopy. Ubiquitin-positive intraneuronal inclusions were seen in the hippocampal granular cell layer and entorhinal cortex of 7 out of the ALS patients. Similar inclusions could not be seen in the same areas in the controls. They were not seen on light microscope staining, nor did they show anilinophilia, argentophilia or congophilia. They were not reacted with other antibodies including neurofilament, tau and paired helical filament (PHF). Immunoelectron microscopy by the preembedding method using anti-ubiquitin antibody showed those inclusions consist of loosely arranged lineal filaments and granular materials. These results suggest that ubiquitin-related cytoskeletal abnormalities are present in cerebral non-motor small neurons in some sporadic ALS patients.

Original languageEnglish (US)
Pages (from-to)233-236
Number of pages4
JournalNeuroscience Letters
Volume129
Issue number2
DOIs
StatePublished - Aug 19 1991
Externally publishedYes

Fingerprint

Amyotrophic Lateral Sclerosis
Motor Cortex
Ubiquitin
Immunoelectron Microscopy
Light
Entorhinal Cortex
Intermediate Filaments
Anti-Idiotypic Antibodies
Electron Microscopy
Staining and Labeling
Neurons
Antibodies
Brain

Keywords

  • Amyotrophic lateral sclerosis
  • Ubiquitin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

New ubiquitin-positive intraneuronal inclusions in the extra-motor cortices in patients with amyotrophic lateral sclerosis. / Okamoto, Koichi; Hirai, Shunsaku; Yamazaki, Tsuneo; Sun, Xiaoyan; Nakazato, Yoichi.

In: Neuroscience Letters, Vol. 129, No. 2, 19.08.1991, p. 233-236.

Research output: Contribution to journalArticle

Okamoto, Koichi ; Hirai, Shunsaku ; Yamazaki, Tsuneo ; Sun, Xiaoyan ; Nakazato, Yoichi. / New ubiquitin-positive intraneuronal inclusions in the extra-motor cortices in patients with amyotrophic lateral sclerosis. In: Neuroscience Letters. 1991 ; Vol. 129, No. 2. pp. 233-236.
@article{e28adacc3ec14051837bd144114652cd,
title = "New ubiquitin-positive intraneuronal inclusions in the extra-motor cortices in patients with amyotrophic lateral sclerosis",
abstract = "We examined the brains of 27 amyotrophic lateral sclerosis (ALS) patients and 50 controls by light, electron and immunoelectron microscopy. Ubiquitin-positive intraneuronal inclusions were seen in the hippocampal granular cell layer and entorhinal cortex of 7 out of the ALS patients. Similar inclusions could not be seen in the same areas in the controls. They were not seen on light microscope staining, nor did they show anilinophilia, argentophilia or congophilia. They were not reacted with other antibodies including neurofilament, tau and paired helical filament (PHF). Immunoelectron microscopy by the preembedding method using anti-ubiquitin antibody showed those inclusions consist of loosely arranged lineal filaments and granular materials. These results suggest that ubiquitin-related cytoskeletal abnormalities are present in cerebral non-motor small neurons in some sporadic ALS patients.",
keywords = "Amyotrophic lateral sclerosis, Ubiquitin",
author = "Koichi Okamoto and Shunsaku Hirai and Tsuneo Yamazaki and Xiaoyan Sun and Yoichi Nakazato",
year = "1991",
month = "8",
day = "19",
doi = "10.1016/0304-3940(91)90469-A",
language = "English (US)",
volume = "129",
pages = "233--236",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - New ubiquitin-positive intraneuronal inclusions in the extra-motor cortices in patients with amyotrophic lateral sclerosis

AU - Okamoto, Koichi

AU - Hirai, Shunsaku

AU - Yamazaki, Tsuneo

AU - Sun, Xiaoyan

AU - Nakazato, Yoichi

PY - 1991/8/19

Y1 - 1991/8/19

N2 - We examined the brains of 27 amyotrophic lateral sclerosis (ALS) patients and 50 controls by light, electron and immunoelectron microscopy. Ubiquitin-positive intraneuronal inclusions were seen in the hippocampal granular cell layer and entorhinal cortex of 7 out of the ALS patients. Similar inclusions could not be seen in the same areas in the controls. They were not seen on light microscope staining, nor did they show anilinophilia, argentophilia or congophilia. They were not reacted with other antibodies including neurofilament, tau and paired helical filament (PHF). Immunoelectron microscopy by the preembedding method using anti-ubiquitin antibody showed those inclusions consist of loosely arranged lineal filaments and granular materials. These results suggest that ubiquitin-related cytoskeletal abnormalities are present in cerebral non-motor small neurons in some sporadic ALS patients.

AB - We examined the brains of 27 amyotrophic lateral sclerosis (ALS) patients and 50 controls by light, electron and immunoelectron microscopy. Ubiquitin-positive intraneuronal inclusions were seen in the hippocampal granular cell layer and entorhinal cortex of 7 out of the ALS patients. Similar inclusions could not be seen in the same areas in the controls. They were not seen on light microscope staining, nor did they show anilinophilia, argentophilia or congophilia. They were not reacted with other antibodies including neurofilament, tau and paired helical filament (PHF). Immunoelectron microscopy by the preembedding method using anti-ubiquitin antibody showed those inclusions consist of loosely arranged lineal filaments and granular materials. These results suggest that ubiquitin-related cytoskeletal abnormalities are present in cerebral non-motor small neurons in some sporadic ALS patients.

KW - Amyotrophic lateral sclerosis

KW - Ubiquitin

UR - http://www.scopus.com/inward/record.url?scp=0025814639&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025814639&partnerID=8YFLogxK

U2 - 10.1016/0304-3940(91)90469-A

DO - 10.1016/0304-3940(91)90469-A

M3 - Article

C2 - 1660578

AN - SCOPUS:0025814639

VL - 129

SP - 233

EP - 236

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 2

ER -