New insight into the inhibition of the inflammatory response to experimental delayed-type hypersensitivity reactions in mice by scropolioside A

Esperanza Bas Infante, M. Carmen Recio, Salvador Máñez, Rosa M. Giner, José M. Escandell, Concha López-Ginés, José Luis Ríos

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Scropolioside A, an iridoid isolated from Scrophularia auriculata ssp. pseudoauriculata, showed anti-inflammatory properties against different experimental models of delayed-type hypersensitivity. This iridoid reduced the oedema induced by oxazolone by 79% (72 h) at 0.5 mg/ear while reducing that induced by sheep red blood cells by 47% (18 h), 45% (24 h) and 36% (48 h) at 10 mg/kg. In vivo it reduced both oedema formation and cell infiltration whereas in vitro it reduced the proliferation of activated T-lymphocytes (IC50 of 67.74 μM). Treatment with scropolioside A (100 μM) 18 and 24 h after phytohemagglutinin stimulation increased the number of cells arrested in the subG0 phase whereas treatment 3 h after stimulation clearly increased the number of cells that passed to the S phase. Scropolioside A also inhibited the production of prostaglandin E2, leukotriene B4, nitric oxide, interleukin-1β, interleukin-2, interleukin-4, tumour necrosis factor-α and interferon-γ, but had no effect on the production of interleukin-10. Moreover, it modified the expression of both nitric oxide synthase-2 and cyclooxygenase-2, as well as the activation of nuclear factor-κB in RAW 264.7 macrophages.

Original languageEnglish (US)
Pages (from-to)199-210
Number of pages12
JournalEuropean Journal of Pharmacology
Volume555
Issue number2-3
DOIs
StatePublished - Jan 26 2007
Externally publishedYes

Fingerprint

Delayed Hypersensitivity
Scrophularia
Edema
Cell Count
Oxazolone
Leukotriene B4
Phytohemagglutinins
Cyclooxygenase 2
Interleukin-1
S Phase
Dinoprostone
Nitric Oxide Synthase
Interleukin-4
Interleukin-10
Interferons
Inhibitory Concentration 50
Interleukin-2
Ear
Sheep
Nitric Oxide

Keywords

  • Anti-inflammatory activity
  • Delayed-type hypersensitivity
  • Iridoid
  • Lymphocyte proliferation
  • Scropolioside A

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

New insight into the inhibition of the inflammatory response to experimental delayed-type hypersensitivity reactions in mice by scropolioside A. / Bas Infante, Esperanza; Recio, M. Carmen; Máñez, Salvador; Giner, Rosa M.; Escandell, José M.; López-Ginés, Concha; Ríos, José Luis.

In: European Journal of Pharmacology, Vol. 555, No. 2-3, 26.01.2007, p. 199-210.

Research output: Contribution to journalArticle

Bas Infante, Esperanza ; Recio, M. Carmen ; Máñez, Salvador ; Giner, Rosa M. ; Escandell, José M. ; López-Ginés, Concha ; Ríos, José Luis. / New insight into the inhibition of the inflammatory response to experimental delayed-type hypersensitivity reactions in mice by scropolioside A. In: European Journal of Pharmacology. 2007 ; Vol. 555, No. 2-3. pp. 199-210.
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