New improved lacZ gene fusion vectors

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

New plasmid vectors suitable for creating fusions with the lacZ gene have been developed. These vectors represent an improvement over currently available vectors and possess the following features: (1) an undetectable background β-galactosidase (βGal) activity in the absence of fusion, (2) an extended multiple cloning site (MCS), and (3) the ability to conveniently subclone in any one of three translational frames. Medium- and high-copy-number versions of these vectors have been developed.

Original languageEnglish (US)
Pages (from-to)99-102
Number of pages4
JournalGene
Volume133
Issue number1
DOIs
StatePublished - Oct 29 1993
Externally publishedYes

Keywords

  • cloning vectors
  • fusion proteins
  • pUC19 Polylinker
  • recombinant DNA
  • transcriptional terminators
  • translational reading frame
  • β-Galactosidase

ASJC Scopus subject areas

  • Genetics

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