New improved lacZ gene fusion vectors

Chaitanya Jain

doi:10.1016/0378-1119(93)90231-Q

New improved lacZ gene fusion vectors

Chaitanya Jain

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

New plasmid vectors suitable for creating fusions with the lacZ gene have been developed. These vectors represent an improvement over currently available vectors and possess the following features: (1) an undetectable background β-galactosidase (βGal) activity in the absence of fusion, (2) an extended multiple cloning site (MCS), and (3) the ability to conveniently subclone in any one of three translational frames. Medium- and high-copy-number versions of these vectors have been developed.

Original language	English (US)
Pages (from-to)	99-102
Number of pages	4
Journal	Gene
Volume	133
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(93)90231-Q
State	Published - Oct 29 1993
Externally published	Yes

Keywords

cloning vectors
fusion proteins
pUC19 Polylinker
recombinant DNA
transcriptional terminators
translational reading frame
β-Galactosidase

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(93)90231-Q

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abstract = "New plasmid vectors suitable for creating fusions with the lacZ gene have been developed. These vectors represent an improvement over currently available vectors and possess the following features: (1) an undetectable background β-galactosidase (βGal) activity in the absence of fusion, (2) an extended multiple cloning site (MCS), and (3) the ability to conveniently subclone in any one of three translational frames. Medium- and high-copy-number versions of these vectors have been developed.",

keywords = "cloning vectors, fusion proteins, pUC19 Polylinker, recombinant DNA, transcriptional terminators, translational reading frame, β-Galactosidase",

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AU - Jain, Chaitanya

PY - 1993/10/29

Y1 - 1993/10/29

N2 - New plasmid vectors suitable for creating fusions with the lacZ gene have been developed. These vectors represent an improvement over currently available vectors and possess the following features: (1) an undetectable background β-galactosidase (βGal) activity in the absence of fusion, (2) an extended multiple cloning site (MCS), and (3) the ability to conveniently subclone in any one of three translational frames. Medium- and high-copy-number versions of these vectors have been developed.

AB - New plasmid vectors suitable for creating fusions with the lacZ gene have been developed. These vectors represent an improvement over currently available vectors and possess the following features: (1) an undetectable background β-galactosidase (βGal) activity in the absence of fusion, (2) an extended multiple cloning site (MCS), and (3) the ability to conveniently subclone in any one of three translational frames. Medium- and high-copy-number versions of these vectors have been developed.

KW - cloning vectors

KW - fusion proteins

KW - pUC19 Polylinker

KW - recombinant DNA

KW - transcriptional terminators

KW - translational reading frame

KW - β-Galactosidase

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DO - 10.1016/0378-1119(93)90231-Q

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VL - 133

SP - 99

EP - 102

JO - Gene

JF - Gene

SN - 0378-1119

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