New improved lacZ gene fusion vectors

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

New plasmid vectors suitable for creating fusions with the lacZ gene have been developed. These vectors represent an improvement over currently available vectors and possess the following features: (1) an undetectable background β-galactosidase (βGal) activity in the absence of fusion, (2) an extended multiple cloning site (MCS), and (3) the ability to conveniently subclone in any one of three translational frames. Medium- and high-copy-number versions of these vectors have been developed.

Original languageEnglish
Pages (from-to)99-102
Number of pages4
JournalGene
Volume133
Issue number1
DOIs
StatePublished - Oct 29 1993
Externally publishedYes

Fingerprint

Galactosidases
Lac Operon
Gene Fusion
Organism Cloning
Plasmids

Keywords

  • cloning vectors
  • fusion proteins
  • pUC19 Polylinker
  • recombinant DNA
  • transcriptional terminators
  • translational reading frame
  • β-Galactosidase

ASJC Scopus subject areas

  • Genetics

Cite this

New improved lacZ gene fusion vectors. / Jain, Chaitanya.

In: Gene, Vol. 133, No. 1, 29.10.1993, p. 99-102.

Research output: Contribution to journalArticle

Jain, Chaitanya. / New improved lacZ gene fusion vectors. In: Gene. 1993 ; Vol. 133, No. 1. pp. 99-102.
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