Abstract
New plasmid vectors suitable for creating fusions with the lacZ gene have been developed. These vectors represent an improvement over currently available vectors and possess the following features: (1) an undetectable background β-galactosidase (βGal) activity in the absence of fusion, (2) an extended multiple cloning site (MCS), and (3) the ability to conveniently subclone in any one of three translational frames. Medium- and high-copy-number versions of these vectors have been developed.
| Original language | English |
|---|---|
| Pages (from-to) | 99-102 |
| Number of pages | 4 |
| Journal | Gene |
| Volume | 133 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 29 1993 |
| Externally published | Yes |
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Keywords
- cloning vectors
- fusion proteins
- pUC19 Polylinker
- recombinant DNA
- transcriptional terminators
- translational reading frame
- β-Galactosidase
ASJC Scopus subject areas
- Genetics
Cite this
New improved lacZ gene fusion vectors. / Jain, Chaitanya.
In: Gene, Vol. 133, No. 1, 29.10.1993, p. 99-102.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - New improved lacZ gene fusion vectors
AU - Jain, Chaitanya
PY - 1993/10/29
Y1 - 1993/10/29
N2 - New plasmid vectors suitable for creating fusions with the lacZ gene have been developed. These vectors represent an improvement over currently available vectors and possess the following features: (1) an undetectable background β-galactosidase (βGal) activity in the absence of fusion, (2) an extended multiple cloning site (MCS), and (3) the ability to conveniently subclone in any one of three translational frames. Medium- and high-copy-number versions of these vectors have been developed.
AB - New plasmid vectors suitable for creating fusions with the lacZ gene have been developed. These vectors represent an improvement over currently available vectors and possess the following features: (1) an undetectable background β-galactosidase (βGal) activity in the absence of fusion, (2) an extended multiple cloning site (MCS), and (3) the ability to conveniently subclone in any one of three translational frames. Medium- and high-copy-number versions of these vectors have been developed.
KW - cloning vectors
KW - fusion proteins
KW - pUC19 Polylinker
KW - recombinant DNA
KW - transcriptional terminators
KW - translational reading frame
KW - β-Galactosidase
UR - http://www.scopus.com/inward/record.url?scp=0027505060&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027505060&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(93)90231-Q
DO - 10.1016/0378-1119(93)90231-Q
M3 - Article
C2 - 8224902
AN - SCOPUS:0027505060
VL - 133
SP - 99
EP - 102
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1
ER -