New improved lacZ gene fusion vectors

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


New plasmid vectors suitable for creating fusions with the lacZ gene have been developed. These vectors represent an improvement over currently available vectors and possess the following features: (1) an undetectable background β-galactosidase (βGal) activity in the absence of fusion, (2) an extended multiple cloning site (MCS), and (3) the ability to conveniently subclone in any one of three translational frames. Medium- and high-copy-number versions of these vectors have been developed.

Original languageEnglish (US)
Pages (from-to)99-102
Number of pages4
Issue number1
StatePublished - Oct 29 1993
Externally publishedYes


  • cloning vectors
  • fusion proteins
  • pUC19 Polylinker
  • recombinant DNA
  • transcriptional terminators
  • translational reading frame
  • β-Galactosidase

ASJC Scopus subject areas

  • Genetics


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