Abstract
New plasmid vectors suitable for creating fusions with the lacZ gene have been developed. These vectors represent an improvement over currently available vectors and possess the following features: (1) an undetectable background β-galactosidase (βGal) activity in the absence of fusion, (2) an extended multiple cloning site (MCS), and (3) the ability to conveniently subclone in any one of three translational frames. Medium- and high-copy-number versions of these vectors have been developed.
Original language | English (US) |
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Pages (from-to) | 99-102 |
Number of pages | 4 |
Journal | Gene |
Volume | 133 |
Issue number | 1 |
DOIs | |
State | Published - Oct 29 1993 |
Externally published | Yes |
Keywords
- cloning vectors
- fusion proteins
- pUC19 Polylinker
- recombinant DNA
- transcriptional terminators
- translational reading frame
- β-Galactosidase
ASJC Scopus subject areas
- Genetics