Abstract
New plasmid vectors suitable for creating fusions with the lacZ gene have been developed. These vectors represent an improvement over currently available vectors and possess the following features: (1) an undetectable background β-galactosidase (βGal) activity in the absence of fusion, (2) an extended multiple cloning site (MCS), and (3) the ability to conveniently subclone in any one of three translational frames. Medium- and high-copy-number versions of these vectors have been developed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 99-102 |
| Number of pages | 4 |
| Journal | Gene |
| Volume | 133 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 29 1993 |
| Externally published | Yes |
Keywords
- cloning vectors
- fusion proteins
- pUC19 Polylinker
- recombinant DNA
- transcriptional terminators
- translational reading frame
- β-Galactosidase
ASJC Scopus subject areas
- Genetics
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Cite this
Jain, C. (1993). New improved lacZ gene fusion vectors. Gene, 133(1), 99-102. https://doi.org/10.1016/0378-1119(93)90231-Q