New and specific nucleoside diphosphate glucose substrates for glycogenin

Miriam D. Alonso, Erik J. Lagzdins, Joseph Lomako, Wieslawa M. Lomako, William J. Whelan

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Glycogenin, the autocatalytic, self-glucosylating primer for glycogen synthesis by glycogen synthase, is presumed, in vivo, to use UDP-glucose as the source of the glucose residues it adds to itself. When we tested its ability to utilize other nucleoside diphosphate glucoses, it emerged that purine nucleotides are not utilized but two pyrimidine nucleotides are used, in addition to UDP-glucose. These are CDP-glucose and TDP-glucose. CDP-glucose is utilized at 70% of the rate of UDP-glucose. While there is no evidence that CDP-glucose is a natural substrate for glycogenin, it has the advantage over UDP-glucose in that it can be used specifically to detect and assay glycogenin in the presence of glycogen synthase because CDP-glucose, unlike UDP-glucose, is not a substrate for the synthase.

Original languageEnglish (US)
Pages (from-to)110-112
Number of pages3
JournalFEBS letters
Volume359
Issue number2-3
DOIs
StatePublished - Feb 13 1995

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Keywords

  • Apo-glycogenin
  • CDP-glucose
  • Glycogenin
  • TDP-glucose

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

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