Abstract
This study investigated the possible contribution of neutrophils to development of reexpansion pulmonary edema (RPE) in rabbits. Rabbits' right lungs were collapsed for 7 days and then reexpanded with negative intrathoracic pressure for 2 h before study, a model that creates unilateral edema in the reexpanded lungs but not in contralateral left lungs. Two hours after lung reexpansion, significant increases in lavage albumin concentration (17-fold), percent neutrophils (14-fold), and total number of neutrophils (7-fold) recovered occurred in the reexpanded lung but not in the left. After 2 h of reexpansion increased leukotriene B4 was detected in lavage supernatant from right lungs (335 ± 33 pg/ml) compared with the left (110 ± 12 pg/mg, P < 0.01), and right lung lavage acid phosphatase activity similarly increased (6.67 ± 0.35 U/l) compared with left (4.73 ± 0.60 U/l, P < 0.05). Neutropenia induced by nitrogen mustard (17 ± 14 > neutrophils/μl) did not prevent RPE, because reexpanded lungs from six neutropenic rabbits were edematous (wet-to-dry lung weight ratio 6.34 ± 0.43) compared with their contralateral lungs (4.97 ± 0.04, P < 0.01). An elevated albumin concentration in reexpanded lung lavage from neutropenic rabbits (8-fold) confirmed an increase in permeability. Neutrophil depletion before reexpansion did not prevent unilateral edema, although neutrophils were absent from lung sections and alveolar lavage fluid from neutropenic rabbits.
| Original language | English |
|---|---|
| Pages (from-to) | 228-234 |
| Number of pages | 7 |
| Journal | Journal of Applied Physiology |
| Volume | 65 |
| Issue number | 1 |
| State | Published - Jan 1 1988 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Endocrinology
- Physiology
- Orthopedics and Sports Medicine
- Physical Therapy, Sports Therapy and Rehabilitation
Cite this
Neutrophils in reexpansion pulmonary edema. / Jackson, Robert; Veal, C. F.; Alexander, C. B.; Brannen, A. L.; Fulmer, J. D.
In: Journal of Applied Physiology, Vol. 65, No. 1, 01.01.1988, p. 228-234.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neutrophils in reexpansion pulmonary edema
AU - Jackson, Robert
AU - Veal, C. F.
AU - Alexander, C. B.
AU - Brannen, A. L.
AU - Fulmer, J. D.
PY - 1988/1/1
Y1 - 1988/1/1
N2 - This study investigated the possible contribution of neutrophils to development of reexpansion pulmonary edema (RPE) in rabbits. Rabbits' right lungs were collapsed for 7 days and then reexpanded with negative intrathoracic pressure for 2 h before study, a model that creates unilateral edema in the reexpanded lungs but not in contralateral left lungs. Two hours after lung reexpansion, significant increases in lavage albumin concentration (17-fold), percent neutrophils (14-fold), and total number of neutrophils (7-fold) recovered occurred in the reexpanded lung but not in the left. After 2 h of reexpansion increased leukotriene B4 was detected in lavage supernatant from right lungs (335 ± 33 pg/ml) compared with the left (110 ± 12 pg/mg, P < 0.01), and right lung lavage acid phosphatase activity similarly increased (6.67 ± 0.35 U/l) compared with left (4.73 ± 0.60 U/l, P < 0.05). Neutropenia induced by nitrogen mustard (17 ± 14 > neutrophils/μl) did not prevent RPE, because reexpanded lungs from six neutropenic rabbits were edematous (wet-to-dry lung weight ratio 6.34 ± 0.43) compared with their contralateral lungs (4.97 ± 0.04, P < 0.01). An elevated albumin concentration in reexpanded lung lavage from neutropenic rabbits (8-fold) confirmed an increase in permeability. Neutrophil depletion before reexpansion did not prevent unilateral edema, although neutrophils were absent from lung sections and alveolar lavage fluid from neutropenic rabbits.
AB - This study investigated the possible contribution of neutrophils to development of reexpansion pulmonary edema (RPE) in rabbits. Rabbits' right lungs were collapsed for 7 days and then reexpanded with negative intrathoracic pressure for 2 h before study, a model that creates unilateral edema in the reexpanded lungs but not in contralateral left lungs. Two hours after lung reexpansion, significant increases in lavage albumin concentration (17-fold), percent neutrophils (14-fold), and total number of neutrophils (7-fold) recovered occurred in the reexpanded lung but not in the left. After 2 h of reexpansion increased leukotriene B4 was detected in lavage supernatant from right lungs (335 ± 33 pg/ml) compared with the left (110 ± 12 pg/mg, P < 0.01), and right lung lavage acid phosphatase activity similarly increased (6.67 ± 0.35 U/l) compared with left (4.73 ± 0.60 U/l, P < 0.05). Neutropenia induced by nitrogen mustard (17 ± 14 > neutrophils/μl) did not prevent RPE, because reexpanded lungs from six neutropenic rabbits were edematous (wet-to-dry lung weight ratio 6.34 ± 0.43) compared with their contralateral lungs (4.97 ± 0.04, P < 0.01). An elevated albumin concentration in reexpanded lung lavage from neutropenic rabbits (8-fold) confirmed an increase in permeability. Neutrophil depletion before reexpansion did not prevent unilateral edema, although neutrophils were absent from lung sections and alveolar lavage fluid from neutropenic rabbits.
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M3 - Article
C2 - 2841277
AN - SCOPUS:0023726083
VL - 65
SP - 228
EP - 234
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 1
ER -