Neutralizing antibodies in sera from macaques immunized with attenuated simian immunodeficiency virus

Alphonse J. Langlois, Ronald Charles Desrosiers, Mark G. Lewis, Vineet N. Kewalramani, Dan R. Littman, Ji Ying Zhou, Kelledy Manson, Michael S. Wyand, Dani P. Bolognesi, David C. Montefiori

Research output: Contribution to journalArticle

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Abstract

Infection with attenuated simian immunodeficiency virus (SIV) in rhesus macaques has been shown to raise antibodies capable of neutralizing an animal challenge stock of primary SIVmac251 in CEMx174 cells that correlate with resistance to infection after experimental challenge with this virulent virus (M. S. Wyand, K. H. Manson, M. Garcia-Moll, D.C. Montefiori, and R. C. Desrosiers, J. Virol. 70:3724-3733, 1996). Here we show that these neutralizing antibodies are not detected in human and rhesus peripheral blood mononuclear cells (PBMC). In addition, neutralization of primary SIVmac251 in human and rhesus PBMC was rarely detected with plasma samples from a similar group of animals that had been infected either with SIVmac239Δnef for 1.5 years or with SIVmac239Δ3 for 3.2 years, although low-level neutralization was detected in CEMx174 cells. Potent neutralization was detected in CEMx174 cells when the latter plasma samples were assessed with laboratory-adapted SIVmac251. In contrast to primary SIVmac251, laboratory-adapted SIVmac251 did not replicate in human and rhesus PBMC despite its ability to utilize CCR5, Bonzo/STRL33, and BOB/gpr15 as coreceptors for virus entry. These results illustrate the importance of virus passage history and the choice of indicator cells for making assessments of neutralizing antibodies to lentiviruses such as SIV. They also demonstrate that primary SIVmac251 is less sensitive to neutralization in human and rhesus PBMC than it is in established cell lines. Results obtained in PBMC did not support a role for neutralizing antibodies as a mechanism of protection in animals immunized with attenuated SIV and challenged with primary SIVmac251.

Original languageEnglish (US)
Pages (from-to)6950-6955
Number of pages6
JournalJournal of Virology
Volume72
Issue number8
StatePublished - Aug 1998
Externally publishedYes

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Simian immunodeficiency virus
Simian Immunodeficiency Virus
Macaca
mononuclear leukocytes
Neutralizing Antibodies
neutralizing antibodies
Blood Cells
neutralization
Serum
viruses
cells
Viruses
Virus Internalization
animals
Lentivirus
Macaca mulatta
Infection
infection
History
cell lines

ASJC Scopus subject areas

  • Immunology

Cite this

Langlois, A. J., Desrosiers, R. C., Lewis, M. G., Kewalramani, V. N., Littman, D. R., Zhou, J. Y., ... Montefiori, D. C. (1998). Neutralizing antibodies in sera from macaques immunized with attenuated simian immunodeficiency virus. Journal of Virology, 72(8), 6950-6955.

Neutralizing antibodies in sera from macaques immunized with attenuated simian immunodeficiency virus. / Langlois, Alphonse J.; Desrosiers, Ronald Charles; Lewis, Mark G.; Kewalramani, Vineet N.; Littman, Dan R.; Zhou, Ji Ying; Manson, Kelledy; Wyand, Michael S.; Bolognesi, Dani P.; Montefiori, David C.

In: Journal of Virology, Vol. 72, No. 8, 08.1998, p. 6950-6955.

Research output: Contribution to journalArticle

Langlois, AJ, Desrosiers, RC, Lewis, MG, Kewalramani, VN, Littman, DR, Zhou, JY, Manson, K, Wyand, MS, Bolognesi, DP & Montefiori, DC 1998, 'Neutralizing antibodies in sera from macaques immunized with attenuated simian immunodeficiency virus', Journal of Virology, vol. 72, no. 8, pp. 6950-6955.
Langlois AJ, Desrosiers RC, Lewis MG, Kewalramani VN, Littman DR, Zhou JY et al. Neutralizing antibodies in sera from macaques immunized with attenuated simian immunodeficiency virus. Journal of Virology. 1998 Aug;72(8):6950-6955.
Langlois, Alphonse J. ; Desrosiers, Ronald Charles ; Lewis, Mark G. ; Kewalramani, Vineet N. ; Littman, Dan R. ; Zhou, Ji Ying ; Manson, Kelledy ; Wyand, Michael S. ; Bolognesi, Dani P. ; Montefiori, David C. / Neutralizing antibodies in sera from macaques immunized with attenuated simian immunodeficiency virus. In: Journal of Virology. 1998 ; Vol. 72, No. 8. pp. 6950-6955.
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