Neutral lipids facilitate transfer of bone morphogenetic proteins and other noncollagenous proteins

M. R. Urist, K. Behnam, F. Kerendi, D. Bagga, H. D. Adkisson, J. Nevenzel, Theodore Malinin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In the process of separation of bone morphogenetic proteins from bone matrix, lipids were found in unexpected amounts closely associated with noncollagenous proteins soluble in guanidine hydrochloride. Lipids representing 33.7-49.9% by weight were recovered with various solvents. Composites of noncollagenous proteins and lipids soluble in either chloroform- methanol or acetone implanted in the hindquarter muscles of mice induced the formation of large deposits of heterotopic bone. The protein-lipid aggregates formed microspherules which were stained by Sudan Black B. Implants of bone morphogenetic proteins and noncollagenous proteins-lipid microspherules stained with Sudan Black B induced bone development in the same manner as unstained delipidized bone morphogenetic proteins associated with noncollagenous proteins. Lipid-free osteocalcein, osteonectin, albumin and other bone matrix proteins did not induce bone formation or bind Sudan Black B. The more highly purified the noncollagenous proteins, with or without activity of bone morphogenetic proteins, the lower the level of binding with Sudan Black B. Acetone-soluble bone matrix lipids consisted chiefly of triglycerides, cholesterol and saturated short chain fatty acids, and included little or no phospholipids or monounsaturated fatty acids. Composites of recombinant bone morphogenetic proteins-2 and acetone-soluble lipids induced larger deposits of bone than implants of recombinant bone morphogenetic proteins-2 without acetone-soluble lipids. The hypothesis that an association of bone lipids with protein facilitates the local transport of bone morphogenetic proteins warrants further investigation.

Original languageEnglish
Pages (from-to)465-475
Number of pages11
JournalMedical Hypotheses
Volume49
Issue number6
DOIs
StatePublished - Dec 1 1997

Fingerprint

Bone Morphogenetic Proteins
Lipids
Acetone
Proteins
Bone Matrix
Bone Morphogenetic Protein 2
Recombinant Proteins
Bone and Bones
Osteonectin
Monounsaturated Fatty Acids
Volatile Fatty Acids
Bone Development
Guanidine
Chloroform
Osteogenesis
Methanol
Albumins
Phospholipids
Triglycerides
Cholesterol

ASJC Scopus subject areas

  • Developmental Biology
  • Medicine(all)
  • Drug Discovery

Cite this

Urist, M. R., Behnam, K., Kerendi, F., Bagga, D., Adkisson, H. D., Nevenzel, J., & Malinin, T. (1997). Neutral lipids facilitate transfer of bone morphogenetic proteins and other noncollagenous proteins. Medical Hypotheses, 49(6), 465-475. https://doi.org/10.1016/S0306-9877(97)90064-5

Neutral lipids facilitate transfer of bone morphogenetic proteins and other noncollagenous proteins. / Urist, M. R.; Behnam, K.; Kerendi, F.; Bagga, D.; Adkisson, H. D.; Nevenzel, J.; Malinin, Theodore.

In: Medical Hypotheses, Vol. 49, No. 6, 01.12.1997, p. 465-475.

Research output: Contribution to journalArticle

Urist, MR, Behnam, K, Kerendi, F, Bagga, D, Adkisson, HD, Nevenzel, J & Malinin, T 1997, 'Neutral lipids facilitate transfer of bone morphogenetic proteins and other noncollagenous proteins', Medical Hypotheses, vol. 49, no. 6, pp. 465-475. https://doi.org/10.1016/S0306-9877(97)90064-5
Urist, M. R. ; Behnam, K. ; Kerendi, F. ; Bagga, D. ; Adkisson, H. D. ; Nevenzel, J. ; Malinin, Theodore. / Neutral lipids facilitate transfer of bone morphogenetic proteins and other noncollagenous proteins. In: Medical Hypotheses. 1997 ; Vol. 49, No. 6. pp. 465-475.
@article{c22934a9d3bf45389992e376ebd1dff3,
title = "Neutral lipids facilitate transfer of bone morphogenetic proteins and other noncollagenous proteins",
abstract = "In the process of separation of bone morphogenetic proteins from bone matrix, lipids were found in unexpected amounts closely associated with noncollagenous proteins soluble in guanidine hydrochloride. Lipids representing 33.7-49.9{\%} by weight were recovered with various solvents. Composites of noncollagenous proteins and lipids soluble in either chloroform- methanol or acetone implanted in the hindquarter muscles of mice induced the formation of large deposits of heterotopic bone. The protein-lipid aggregates formed microspherules which were stained by Sudan Black B. Implants of bone morphogenetic proteins and noncollagenous proteins-lipid microspherules stained with Sudan Black B induced bone development in the same manner as unstained delipidized bone morphogenetic proteins associated with noncollagenous proteins. Lipid-free osteocalcein, osteonectin, albumin and other bone matrix proteins did not induce bone formation or bind Sudan Black B. The more highly purified the noncollagenous proteins, with or without activity of bone morphogenetic proteins, the lower the level of binding with Sudan Black B. Acetone-soluble bone matrix lipids consisted chiefly of triglycerides, cholesterol and saturated short chain fatty acids, and included little or no phospholipids or monounsaturated fatty acids. Composites of recombinant bone morphogenetic proteins-2 and acetone-soluble lipids induced larger deposits of bone than implants of recombinant bone morphogenetic proteins-2 without acetone-soluble lipids. The hypothesis that an association of bone lipids with protein facilitates the local transport of bone morphogenetic proteins warrants further investigation.",
author = "Urist, {M. R.} and K. Behnam and F. Kerendi and D. Bagga and Adkisson, {H. D.} and J. Nevenzel and Theodore Malinin",
year = "1997",
month = "12",
day = "1",
doi = "10.1016/S0306-9877(97)90064-5",
language = "English",
volume = "49",
pages = "465--475",
journal = "Medical Hypotheses",
issn = "0306-9877",
publisher = "Churchill Livingstone",
number = "6",

}

TY - JOUR

T1 - Neutral lipids facilitate transfer of bone morphogenetic proteins and other noncollagenous proteins

AU - Urist, M. R.

AU - Behnam, K.

AU - Kerendi, F.

AU - Bagga, D.

AU - Adkisson, H. D.

AU - Nevenzel, J.

AU - Malinin, Theodore

PY - 1997/12/1

Y1 - 1997/12/1

N2 - In the process of separation of bone morphogenetic proteins from bone matrix, lipids were found in unexpected amounts closely associated with noncollagenous proteins soluble in guanidine hydrochloride. Lipids representing 33.7-49.9% by weight were recovered with various solvents. Composites of noncollagenous proteins and lipids soluble in either chloroform- methanol or acetone implanted in the hindquarter muscles of mice induced the formation of large deposits of heterotopic bone. The protein-lipid aggregates formed microspherules which were stained by Sudan Black B. Implants of bone morphogenetic proteins and noncollagenous proteins-lipid microspherules stained with Sudan Black B induced bone development in the same manner as unstained delipidized bone morphogenetic proteins associated with noncollagenous proteins. Lipid-free osteocalcein, osteonectin, albumin and other bone matrix proteins did not induce bone formation or bind Sudan Black B. The more highly purified the noncollagenous proteins, with or without activity of bone morphogenetic proteins, the lower the level of binding with Sudan Black B. Acetone-soluble bone matrix lipids consisted chiefly of triglycerides, cholesterol and saturated short chain fatty acids, and included little or no phospholipids or monounsaturated fatty acids. Composites of recombinant bone morphogenetic proteins-2 and acetone-soluble lipids induced larger deposits of bone than implants of recombinant bone morphogenetic proteins-2 without acetone-soluble lipids. The hypothesis that an association of bone lipids with protein facilitates the local transport of bone morphogenetic proteins warrants further investigation.

AB - In the process of separation of bone morphogenetic proteins from bone matrix, lipids were found in unexpected amounts closely associated with noncollagenous proteins soluble in guanidine hydrochloride. Lipids representing 33.7-49.9% by weight were recovered with various solvents. Composites of noncollagenous proteins and lipids soluble in either chloroform- methanol or acetone implanted in the hindquarter muscles of mice induced the formation of large deposits of heterotopic bone. The protein-lipid aggregates formed microspherules which were stained by Sudan Black B. Implants of bone morphogenetic proteins and noncollagenous proteins-lipid microspherules stained with Sudan Black B induced bone development in the same manner as unstained delipidized bone morphogenetic proteins associated with noncollagenous proteins. Lipid-free osteocalcein, osteonectin, albumin and other bone matrix proteins did not induce bone formation or bind Sudan Black B. The more highly purified the noncollagenous proteins, with or without activity of bone morphogenetic proteins, the lower the level of binding with Sudan Black B. Acetone-soluble bone matrix lipids consisted chiefly of triglycerides, cholesterol and saturated short chain fatty acids, and included little or no phospholipids or monounsaturated fatty acids. Composites of recombinant bone morphogenetic proteins-2 and acetone-soluble lipids induced larger deposits of bone than implants of recombinant bone morphogenetic proteins-2 without acetone-soluble lipids. The hypothesis that an association of bone lipids with protein facilitates the local transport of bone morphogenetic proteins warrants further investigation.

UR - http://www.scopus.com/inward/record.url?scp=0031459367&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031459367&partnerID=8YFLogxK

U2 - 10.1016/S0306-9877(97)90064-5

DO - 10.1016/S0306-9877(97)90064-5

M3 - Article

C2 - 9466369

AN - SCOPUS:0031459367

VL - 49

SP - 465

EP - 475

JO - Medical Hypotheses

JF - Medical Hypotheses

SN - 0306-9877

IS - 6

ER -