Neurotrophic effects of BDNF and CNTF, alone and in combination, on postnatal day 5 rat acoustic ganglion neurons

C. J. Hartnick, H. Staecker, B. Malgrange, P. P. Lefebvre, W. Liu, G. Moonen, T. R. Van De Water

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

The neuronal survival promoting ability of brain derived neurotrophic factor (BDNF), and ciliary neurotrophic factor (CNTF), individually and in combination, was evaluated in dissociated cell cultures of postnatal day 5 (PS) rat acoustic ganglia. The neuritogenic promoting effect of these same neurotrophic factors was examined in organotypic explants of P5 rat acoustic ganglia. The results showed that BDNF was maximally effective at a concentration of 10 ng/mL in promoting both survival and neuritogenesis of these postnatal auditory neurons in vitro. CNTF was maximally effective at a concentration of 0.01 ng/mL at promoting both survival and neuritogenesis in the acoustic ganglion cultures. BDNF had its strongest effect on neuronal survival while CNTF was most effective in stimulating neurite outgrowth. These two neurotrophic factors, when added together at their respective maximally effective concentrations, behave in an additive manner for promoting both survival and neuritic outgrowth by the auditory neurons.

Original languageEnglish (US)
Pages (from-to)246-254
Number of pages9
JournalJournal of Neurobiology
Volume30
Issue number2
DOIs
StatePublished - Jun 1 1996

Keywords

  • additive effect
  • auditory neurons
  • cytokine
  • in vitro
  • neurotrophin

ASJC Scopus subject areas

  • Neuroscience(all)

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