Neurotrophic and neuroimmune responses to early-life Pseudomonas aeruginosa infection in rat lungs

Silvia Cardenas, Mario Scuri, Lennie Samsell, Barbara Ducatman, Pablo Bejarano, Alexander Auais, Melissa Doud, Kalai Mathee, Giovanni Piedimonte

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Early-life respiratory infection with Pseudomonas aeruginosa is common in children with cystic fibrosis or immune deficits. Although many of its clinical manifestations involve neural reflexes, little information is available on the peripheral nervous system of infected airways. This study sought to determine whether early-life infection triggers a neurogenic-mediated immunoinflammatory response, the mechanisms of this response, and its relationship with other immunoinflammatory pathways. Weanling and adult rats were inoculated with suspensions containing P. aeruginosa (PAO1) coated on alginate microspheres suspended in Tris-CaCl2 buffer. Five days after infection, rats were injected with capsaicin to stimulate nociceptive nerves in the airway mucosa, and microvascular permeability was measured using Evans blue as a tracer. PAO1 increased neurogenic inflammation in the extra- and intrapulmonary compartments of weanlings but not in adults. The mechanism involves selective overexpression of NGF, which is critical for the local increase in microvascular permeability and for the infiltration of polymorphonuclear leukocytes into infected lung parenchyma. These effects are mediated in part by induction of downstream inflammatory cytokines and chemokines, especially IL-1β, IL-18, and leptin. Our data suggest that neurogenic-mediated immunoinflammatory mechanisms play important roles in airway inflammation and hyperreactivity associated with P. aeruginosa when infection occurs early in life.

Original languageEnglish (US)
Pages (from-to)L334-L344
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume299
Issue number3
DOIs
StatePublished - Sep 1 2010

Keywords

  • Cystic fibrosis
  • Immunodeficiency
  • Nerve growth factor
  • Neuroimmunomodulation

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology
  • Physiology
  • Medicine(all)

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    Cardenas, S., Scuri, M., Samsell, L., Ducatman, B., Bejarano, P., Auais, A., Doud, M., Mathee, K., & Piedimonte, G. (2010). Neurotrophic and neuroimmune responses to early-life Pseudomonas aeruginosa infection in rat lungs. American Journal of Physiology - Lung Cellular and Molecular Physiology, 299(3), L334-L344. https://doi.org/10.1152/ajplung.00017.2010