Abstract
Early-life respiratory infection with Pseudomonas aeruginosa is common in children with cystic fibrosis or immune deficits. Although many of its clinical manifestations involve neural reflexes, little information is available on the peripheral nervous system of infected airways. This study sought to determine whether early-life infection triggers a neurogenic-mediated immunoinflammatory response, the mechanisms of this response, and its relationship with other immunoinflammatory pathways. Weanling and adult rats were inoculated with suspensions containing P. aeruginosa (PAO1) coated on alginate microspheres suspended in Tris-CaCl2 buffer. Five days after infection, rats were injected with capsaicin to stimulate nociceptive nerves in the airway mucosa, and microvascular permeability was measured using Evans blue as a tracer. PAO1 increased neurogenic inflammation in the extra- and intrapulmonary compartments of weanlings but not in adults. The mechanism involves selective overexpression of NGF, which is critical for the local increase in microvascular permeability and for the infiltration of polymorphonuclear leukocytes into infected lung parenchyma. These effects are mediated in part by induction of downstream inflammatory cytokines and chemokines, especially IL-1β, IL-18, and leptin. Our data suggest that neurogenic-mediated immunoinflammatory mechanisms play important roles in airway inflammation and hyperreactivity associated with P. aeruginosa when infection occurs early in life.
| Original language | English |
|---|---|
| Journal | American Journal of Physiology - Lung Cellular and Molecular Physiology |
| Volume | 299 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 1 2010 |
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Keywords
- Cystic fibrosis
- Immunodeficiency
- Nerve growth factor
- Neuroimmunomodulation
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
- Physiology (medical)
- Cell Biology
- Physiology
- Medicine(all)
Cite this
Neurotrophic and neuroimmune responses to early-life Pseudomonas aeruginosa infection in rat lungs. / Cardenas, Silvia; Scuri, Mario; Samsell, Lennie; Ducatman, Barbara; Bejarano, Pablo; Auais, Alexander; Doud, Melissa; Mathee, Kalai; Piedimonte, Giovanni.
In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 299, No. 3, 01.09.2010.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neurotrophic and neuroimmune responses to early-life Pseudomonas aeruginosa infection in rat lungs
AU - Cardenas, Silvia
AU - Scuri, Mario
AU - Samsell, Lennie
AU - Ducatman, Barbara
AU - Bejarano, Pablo
AU - Auais, Alexander
AU - Doud, Melissa
AU - Mathee, Kalai
AU - Piedimonte, Giovanni
PY - 2010/9/1
Y1 - 2010/9/1
N2 - Early-life respiratory infection with Pseudomonas aeruginosa is common in children with cystic fibrosis or immune deficits. Although many of its clinical manifestations involve neural reflexes, little information is available on the peripheral nervous system of infected airways. This study sought to determine whether early-life infection triggers a neurogenic-mediated immunoinflammatory response, the mechanisms of this response, and its relationship with other immunoinflammatory pathways. Weanling and adult rats were inoculated with suspensions containing P. aeruginosa (PAO1) coated on alginate microspheres suspended in Tris-CaCl2 buffer. Five days after infection, rats were injected with capsaicin to stimulate nociceptive nerves in the airway mucosa, and microvascular permeability was measured using Evans blue as a tracer. PAO1 increased neurogenic inflammation in the extra- and intrapulmonary compartments of weanlings but not in adults. The mechanism involves selective overexpression of NGF, which is critical for the local increase in microvascular permeability and for the infiltration of polymorphonuclear leukocytes into infected lung parenchyma. These effects are mediated in part by induction of downstream inflammatory cytokines and chemokines, especially IL-1β, IL-18, and leptin. Our data suggest that neurogenic-mediated immunoinflammatory mechanisms play important roles in airway inflammation and hyperreactivity associated with P. aeruginosa when infection occurs early in life.
AB - Early-life respiratory infection with Pseudomonas aeruginosa is common in children with cystic fibrosis or immune deficits. Although many of its clinical manifestations involve neural reflexes, little information is available on the peripheral nervous system of infected airways. This study sought to determine whether early-life infection triggers a neurogenic-mediated immunoinflammatory response, the mechanisms of this response, and its relationship with other immunoinflammatory pathways. Weanling and adult rats were inoculated with suspensions containing P. aeruginosa (PAO1) coated on alginate microspheres suspended in Tris-CaCl2 buffer. Five days after infection, rats were injected with capsaicin to stimulate nociceptive nerves in the airway mucosa, and microvascular permeability was measured using Evans blue as a tracer. PAO1 increased neurogenic inflammation in the extra- and intrapulmonary compartments of weanlings but not in adults. The mechanism involves selective overexpression of NGF, which is critical for the local increase in microvascular permeability and for the infiltration of polymorphonuclear leukocytes into infected lung parenchyma. These effects are mediated in part by induction of downstream inflammatory cytokines and chemokines, especially IL-1β, IL-18, and leptin. Our data suggest that neurogenic-mediated immunoinflammatory mechanisms play important roles in airway inflammation and hyperreactivity associated with P. aeruginosa when infection occurs early in life.
KW - Cystic fibrosis
KW - Immunodeficiency
KW - Nerve growth factor
KW - Neuroimmunomodulation
UR - http://www.scopus.com/inward/record.url?scp=77956674294&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77956674294&partnerID=8YFLogxK
U2 - 10.1152/ajplung.00017.2010
DO - 10.1152/ajplung.00017.2010
M3 - Article
C2 - 20543002
AN - SCOPUS:77956674294
VL - 299
JO - American Journal of Physiology - Cell Physiology
JF - American Journal of Physiology - Cell Physiology
SN - 0363-6143
IS - 3
ER -