Neurotransmitter receptor alterations in Huntington's disease: Auoradiographic and homogenate studies with special reference to benzodiazepine receptor complexes

P. J. Whitehouse, R. R. Trifiletti, B. E. Jones, S. Folstein, D. L. Price, S. H. Snyder, M. J. Kuhar

Research output: Contribution to journalArticle

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Abstract

In vitro receptor autoradiography was used to construct semiquantitative maps of subtypes of muscarinic cholinergic (labeled with [3H]N-methylscopolamine), benzodiazepine ([3H]flunitrazepam), γ-aminobutyric acid ([3H]muscimol), dopamine, and serotonin ([3H]spiperone) receptors in frontal cortex, parietal cortex, caudate, putamen, and globus pallidus in tissue sections from 5 patients with clinically well-evaluated Huntington's disease and 5 controls matched with respect to age, sex, and postmortem delay. Homogenates were prepared from the remaining cortical and striatal tissue and used to characterize pharmacologically these same receptors, as well as histamine, adenosine, and nitrendipine receptors. Neuronal loss and gliosis were assessed in the contralateral formalin-fixed caudate and putamen. All binding sites measured (except serotonin) were reduced relative to control values in striatum primarily because of changes in the number of receptor rather than in affinity. Autoradiographic studies generally revealed that these changes were greater in the caudate than the putamen, paralleling the more severe neuropathological changes present in the caudate. In addition, autoradiographic studies demonstrated an increase in γ-aminobutyric acid-related receptors in the globus pallidus. In the cortex, receptor alterations were limited to an increase in the number of benzodiazepine receptors in the frontal cortex which was most prominent in superficial cortical layers.

Original languageEnglish
Pages (from-to)202-210
Number of pages9
JournalAnnals of Neurology
Volume18
Issue number2
DOIs
StatePublished - Oct 31 1985

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Neurotransmitter Receptor
Putamen
Huntington Disease
GABA-A Receptors
Aminobutyrates
Globus Pallidus
Frontal Lobe
Cholinergic Agents
Serotonin
N-Methylscopolamine
Nitrendipine
Flunitrazepam
Corpus Striatum
Muscimol
Parietal Lobe
Purinergic P1 Receptors
Gliosis
Autoradiography
Benzodiazepines
Histamine

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Neurotransmitter receptor alterations in Huntington's disease : Auoradiographic and homogenate studies with special reference to benzodiazepine receptor complexes. / Whitehouse, P. J.; Trifiletti, R. R.; Jones, B. E.; Folstein, S.; Price, D. L.; Snyder, S. H.; Kuhar, M. J.

In: Annals of Neurology, Vol. 18, No. 2, 31.10.1985, p. 202-210.

Research output: Contribution to journalArticle

Whitehouse, P. J. ; Trifiletti, R. R. ; Jones, B. E. ; Folstein, S. ; Price, D. L. ; Snyder, S. H. ; Kuhar, M. J. / Neurotransmitter receptor alterations in Huntington's disease : Auoradiographic and homogenate studies with special reference to benzodiazepine receptor complexes. In: Annals of Neurology. 1985 ; Vol. 18, No. 2. pp. 202-210.
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