Neurotoxic effects of dietary aluminium.

Richard S Jope, G. V. Johnson

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Neurochemical responses to chronic oral aluminium administration have been studied in rats. Aluminium (0.3%) was added to drinking water of adult rats for four weeks or longer and weanling rats were given aluminium for eight weeks. Selective cognitive impairment was demonstrated in the adult rats. Aluminium inhibited calcium flux and phosphoinositide metabolism, one product of which (inositol 1,4,5-trisphosphate) modulates intracellular calcium levels. In weanling rats aluminium decreased the in vivo concentration of inositol 1,4,5-trisphosphate in the hippocampus. An increase in cyclic AMP concentrations by 30-70% in various brain regions in adult and weanling rats was found. Aluminium enhanced agonist-stimulated but not basal cyclic AMP production in vitro. It was postulated that aluminium inhibits the GTPase activity of the stimulatory G protein, Gs, leading to prolonged activation of Gs after receptor stimulation and increased cyclic AMP production. Aluminium treatment also increased the phosphorylation of microtubule-associated protein 2 (MAP-2) and the 200 kDa neurofilament protein (NF-H) but several other phosphoproteins were unaffected. Concentrations of seven structural proteins--MAP-2, tau, NF-H, NF-M (150 kDa), NF-L (68 kDa), tubulin and spectrin--were measured in rat brain regions by immunoblot methods. MAP-2 was most consistently decreased. These studies show that chronic oral aluminium administration to rats has significant neurochemical consequences. Three sites of action are implicated: altered calcium homeostasis, enhanced cyclic AMP production, and changes in cytoskeletal protein phosphorylation states and concentrations.

Original languageEnglish
Pages (from-to)254-262; discussion 262
JournalCiba Foundation symposium
Volume169
StatePublished - Dec 1 1992
Externally publishedYes

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Aluminum
Cyclic AMP
Microtubule-Associated Proteins
Inositol 1,4,5-Trisphosphate
Calcium
Oral Administration
Gs GTP-Binding Protein alpha Subunits
Phosphorylation
Spectrin
Cytoskeletal Proteins
GTP Phosphohydrolases
Phosphoproteins
Brain
Tubulin
Phosphatidylinositols
Drinking Water
Hippocampus
Homeostasis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Jope, R. S., & Johnson, G. V. (1992). Neurotoxic effects of dietary aluminium. Ciba Foundation symposium, 169, 254-262; discussion 262.

Neurotoxic effects of dietary aluminium. / Jope, Richard S; Johnson, G. V.

In: Ciba Foundation symposium, Vol. 169, 01.12.1992, p. 254-262; discussion 262.

Research output: Contribution to journalArticle

Jope, RS & Johnson, GV 1992, 'Neurotoxic effects of dietary aluminium.', Ciba Foundation symposium, vol. 169, pp. 254-262; discussion 262.
Jope RS, Johnson GV. Neurotoxic effects of dietary aluminium. Ciba Foundation symposium. 1992 Dec 1;169:254-262; discussion 262.
Jope, Richard S ; Johnson, G. V. / Neurotoxic effects of dietary aluminium. In: Ciba Foundation symposium. 1992 ; Vol. 169. pp. 254-262; discussion 262.
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