Neurotoxic components in normal serum

JoséE García, Doris Nonner, David Ross, John Barrett

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Serum neurotoxicity was studied by adding whole or fractionated serum (adult human, adult horse, or newborn calf) to neuron-rich cultures prepared from various regions of embryonic (Days 14-15) rat brain, including spinal cord, ventral mesencephalon, cerebellum, septum, and striatum. Effects of serum were also tested on several types of embryonic non-neuronal cells (skeletal muscle myotubes, cardiac muscle myocytes, and fibroblasts from skin and lung). Serum concentrations of 50% or more killed more than 95% of all neurons within 3 days. Serum concentrations as low as 10% also killed some neurons, especially those from cerebellum. Septal, cerebellar, and spinal cord neurons were more sensitive than striatal or mesencephalic neurons. All the tested non-neuronal cells survived much better than neurons at serum concentrations of 20% or more. Neurotoxicity was present in both fresh (human) and previously frozen (human and animal) sera, and affected both young (4 days in vitro) and older (42 days in vitro) cultures. Neurotoxicity was greatly diminished by heating the serum to 56°C for 30 min. Experiments indicated that serum toxicity was not due to lipoprotein, complement, or tumor necrosis factor. All serum neurotoxicity was retained by an ultrafilter with a nominal molecular weight cutoff of 10 kDa. The profile of neurotoxicity following gel filtration at neutral pH was variable, with high toxicity most consistently observed in fractions with apparent molecular weights exceeding 100 kDa, and variable degrees of toxicity at lower molecular weights.

Original languageEnglish
Pages (from-to)309-316
Number of pages8
JournalExperimental Neurology
Volume118
Issue number3
DOIs
StatePublished - Jan 1 1992

Fingerprint

Serum
Neurons
Molecular Weight
Cerebellum
Spinal Cord
Corpus Striatum
Skeletal Muscle Fibers
Mesencephalon
Cardiac Myocytes
Heating
Lipoproteins
Horses
Gel Chromatography
Myocardium
Skeletal Muscle
Tumor Necrosis Factor-alpha
Fibroblasts
Newborn Infant
Lung
Skin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Neurology

Cite this

Neurotoxic components in normal serum. / García, JoséE; Nonner, Doris; Ross, David; Barrett, John.

In: Experimental Neurology, Vol. 118, No. 3, 01.01.1992, p. 309-316.

Research output: Contribution to journalArticle

García, JoséE ; Nonner, Doris ; Ross, David ; Barrett, John. / Neurotoxic components in normal serum. In: Experimental Neurology. 1992 ; Vol. 118, No. 3. pp. 309-316.
@article{7ed36e4deca548038254bb71ec55a22f,
title = "Neurotoxic components in normal serum",
abstract = "Serum neurotoxicity was studied by adding whole or fractionated serum (adult human, adult horse, or newborn calf) to neuron-rich cultures prepared from various regions of embryonic (Days 14-15) rat brain, including spinal cord, ventral mesencephalon, cerebellum, septum, and striatum. Effects of serum were also tested on several types of embryonic non-neuronal cells (skeletal muscle myotubes, cardiac muscle myocytes, and fibroblasts from skin and lung). Serum concentrations of 50{\%} or more killed more than 95{\%} of all neurons within 3 days. Serum concentrations as low as 10{\%} also killed some neurons, especially those from cerebellum. Septal, cerebellar, and spinal cord neurons were more sensitive than striatal or mesencephalic neurons. All the tested non-neuronal cells survived much better than neurons at serum concentrations of 20{\%} or more. Neurotoxicity was present in both fresh (human) and previously frozen (human and animal) sera, and affected both young (4 days in vitro) and older (42 days in vitro) cultures. Neurotoxicity was greatly diminished by heating the serum to 56°C for 30 min. Experiments indicated that serum toxicity was not due to lipoprotein, complement, or tumor necrosis factor. All serum neurotoxicity was retained by an ultrafilter with a nominal molecular weight cutoff of 10 kDa. The profile of neurotoxicity following gel filtration at neutral pH was variable, with high toxicity most consistently observed in fractions with apparent molecular weights exceeding 100 kDa, and variable degrees of toxicity at lower molecular weights.",
author = "Jos{\'e}E Garc{\'i}a and Doris Nonner and David Ross and John Barrett",
year = "1992",
month = "1",
day = "1",
doi = "10.1016/0014-4886(92)90188-V",
language = "English",
volume = "118",
pages = "309--316",
journal = "Experimental Neurology",
issn = "0014-4886",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Neurotoxic components in normal serum

AU - García, JoséE

AU - Nonner, Doris

AU - Ross, David

AU - Barrett, John

PY - 1992/1/1

Y1 - 1992/1/1

N2 - Serum neurotoxicity was studied by adding whole or fractionated serum (adult human, adult horse, or newborn calf) to neuron-rich cultures prepared from various regions of embryonic (Days 14-15) rat brain, including spinal cord, ventral mesencephalon, cerebellum, septum, and striatum. Effects of serum were also tested on several types of embryonic non-neuronal cells (skeletal muscle myotubes, cardiac muscle myocytes, and fibroblasts from skin and lung). Serum concentrations of 50% or more killed more than 95% of all neurons within 3 days. Serum concentrations as low as 10% also killed some neurons, especially those from cerebellum. Septal, cerebellar, and spinal cord neurons were more sensitive than striatal or mesencephalic neurons. All the tested non-neuronal cells survived much better than neurons at serum concentrations of 20% or more. Neurotoxicity was present in both fresh (human) and previously frozen (human and animal) sera, and affected both young (4 days in vitro) and older (42 days in vitro) cultures. Neurotoxicity was greatly diminished by heating the serum to 56°C for 30 min. Experiments indicated that serum toxicity was not due to lipoprotein, complement, or tumor necrosis factor. All serum neurotoxicity was retained by an ultrafilter with a nominal molecular weight cutoff of 10 kDa. The profile of neurotoxicity following gel filtration at neutral pH was variable, with high toxicity most consistently observed in fractions with apparent molecular weights exceeding 100 kDa, and variable degrees of toxicity at lower molecular weights.

AB - Serum neurotoxicity was studied by adding whole or fractionated serum (adult human, adult horse, or newborn calf) to neuron-rich cultures prepared from various regions of embryonic (Days 14-15) rat brain, including spinal cord, ventral mesencephalon, cerebellum, septum, and striatum. Effects of serum were also tested on several types of embryonic non-neuronal cells (skeletal muscle myotubes, cardiac muscle myocytes, and fibroblasts from skin and lung). Serum concentrations of 50% or more killed more than 95% of all neurons within 3 days. Serum concentrations as low as 10% also killed some neurons, especially those from cerebellum. Septal, cerebellar, and spinal cord neurons were more sensitive than striatal or mesencephalic neurons. All the tested non-neuronal cells survived much better than neurons at serum concentrations of 20% or more. Neurotoxicity was present in both fresh (human) and previously frozen (human and animal) sera, and affected both young (4 days in vitro) and older (42 days in vitro) cultures. Neurotoxicity was greatly diminished by heating the serum to 56°C for 30 min. Experiments indicated that serum toxicity was not due to lipoprotein, complement, or tumor necrosis factor. All serum neurotoxicity was retained by an ultrafilter with a nominal molecular weight cutoff of 10 kDa. The profile of neurotoxicity following gel filtration at neutral pH was variable, with high toxicity most consistently observed in fractions with apparent molecular weights exceeding 100 kDa, and variable degrees of toxicity at lower molecular weights.

UR - http://www.scopus.com/inward/record.url?scp=0027066761&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027066761&partnerID=8YFLogxK

U2 - 10.1016/0014-4886(92)90188-V

DO - 10.1016/0014-4886(92)90188-V

M3 - Article

C2 - 1306488

AN - SCOPUS:0027066761

VL - 118

SP - 309

EP - 316

JO - Experimental Neurology

JF - Experimental Neurology

SN - 0014-4886

IS - 3

ER -