Neurotensin-sensitive protein phosphorylation in the rodent caudate nucleus

Scott T. Cain, Charles B. Nemeroff

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Cain, Scott T. and Charles B. Nemeroff: Neurotensin-Sensitive Protein Phosphorylation in the Rodent Caudate Nucleus. Prog. Neuro-Psychopharmacol. & Biol. Psychiat. 1991, 15: 83-89. 1. 1. Caudate nucleus slices from rat brain were prepared and incubated with 5 μM neurotensin for 30 seconds and 5 minutes. Following homogenization of the caudate nucleus slices, proteins were phosphorylated in vitro in the presence of CaCl2 or cyclic AMP. Phosphorylated proteins were separated by electrophoresis, and phosphate incorporation into individual proteins quantitated by microdensitometry of the resultant autoradiographs. 2. 2. Incubation of caudate nucleus slices with neurotensin altered the subsequent in vitro calcium-dependent phosphorylation of several specific protein substrates, but in contrast, incubation with neurotensin altered the subsequent cyclic AMP-dependent phosphorylation of only 1 minor phosphoprotein substrate. 3. 3. These results are consistent with other evidence which implicates calcium as an important intracellular mediator of the neurotensin signal.

Original languageEnglish (US)
Pages (from-to)83-89
Number of pages7
JournalProgress in Neuropsychopharmacology and Biological Psychiatry
Issue number1
StatePublished - 1991
Externally publishedYes


  • brain slice
  • calcium
  • cAMP
  • caudate nucleus
  • neurotensin
  • protein phosphorylation

ASJC Scopus subject areas

  • Biological Psychiatry
  • Pharmacology


Dive into the research topics of 'Neurotensin-sensitive protein phosphorylation in the rodent caudate nucleus'. Together they form a unique fingerprint.

Cite this