Abstract
Cain, Scott T. and Charles B. Nemeroff: Neurotensin-Sensitive Protein Phosphorylation in the Rodent Caudate Nucleus. Prog. Neuro-Psychopharmacol. & Biol. Psychiat. 1991, 15: 83-89. 1. 1. Caudate nucleus slices from rat brain were prepared and incubated with 5 μM neurotensin for 30 seconds and 5 minutes. Following homogenization of the caudate nucleus slices, proteins were phosphorylated in vitro in the presence of CaCl2 or cyclic AMP. Phosphorylated proteins were separated by electrophoresis, and phosphate incorporation into individual proteins quantitated by microdensitometry of the resultant autoradiographs. 2. 2. Incubation of caudate nucleus slices with neurotensin altered the subsequent in vitro calcium-dependent phosphorylation of several specific protein substrates, but in contrast, incubation with neurotensin altered the subsequent cyclic AMP-dependent phosphorylation of only 1 minor phosphoprotein substrate. 3. 3. These results are consistent with other evidence which implicates calcium as an important intracellular mediator of the neurotensin signal.
| Original language | English |
|---|---|
| Pages (from-to) | 83-89 |
| Number of pages | 7 |
| Journal | Progress in Neuropsychopharmacology and Biological Psychiatry |
| Volume | 15 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1991 |
| Externally published | Yes |
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Keywords
- brain slice
- calcium
- cAMP
- caudate nucleus
- neurotensin
- protein phosphorylation
ASJC Scopus subject areas
- Biological Psychiatry
- Pharmacology
Cite this
Neurotensin-sensitive protein phosphorylation in the rodent caudate nucleus. / Cain, Scott T.; Nemeroff, Charles.
In: Progress in Neuropsychopharmacology and Biological Psychiatry, Vol. 15, No. 1, 01.01.1991, p. 83-89.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neurotensin-sensitive protein phosphorylation in the rodent caudate nucleus
AU - Cain, Scott T.
AU - Nemeroff, Charles
PY - 1991/1/1
Y1 - 1991/1/1
N2 - Cain, Scott T. and Charles B. Nemeroff: Neurotensin-Sensitive Protein Phosphorylation in the Rodent Caudate Nucleus. Prog. Neuro-Psychopharmacol. & Biol. Psychiat. 1991, 15: 83-89. 1. 1. Caudate nucleus slices from rat brain were prepared and incubated with 5 μM neurotensin for 30 seconds and 5 minutes. Following homogenization of the caudate nucleus slices, proteins were phosphorylated in vitro in the presence of CaCl2 or cyclic AMP. Phosphorylated proteins were separated by electrophoresis, and phosphate incorporation into individual proteins quantitated by microdensitometry of the resultant autoradiographs. 2. 2. Incubation of caudate nucleus slices with neurotensin altered the subsequent in vitro calcium-dependent phosphorylation of several specific protein substrates, but in contrast, incubation with neurotensin altered the subsequent cyclic AMP-dependent phosphorylation of only 1 minor phosphoprotein substrate. 3. 3. These results are consistent with other evidence which implicates calcium as an important intracellular mediator of the neurotensin signal.
AB - Cain, Scott T. and Charles B. Nemeroff: Neurotensin-Sensitive Protein Phosphorylation in the Rodent Caudate Nucleus. Prog. Neuro-Psychopharmacol. & Biol. Psychiat. 1991, 15: 83-89. 1. 1. Caudate nucleus slices from rat brain were prepared and incubated with 5 μM neurotensin for 30 seconds and 5 minutes. Following homogenization of the caudate nucleus slices, proteins were phosphorylated in vitro in the presence of CaCl2 or cyclic AMP. Phosphorylated proteins were separated by electrophoresis, and phosphate incorporation into individual proteins quantitated by microdensitometry of the resultant autoradiographs. 2. 2. Incubation of caudate nucleus slices with neurotensin altered the subsequent in vitro calcium-dependent phosphorylation of several specific protein substrates, but in contrast, incubation with neurotensin altered the subsequent cyclic AMP-dependent phosphorylation of only 1 minor phosphoprotein substrate. 3. 3. These results are consistent with other evidence which implicates calcium as an important intracellular mediator of the neurotensin signal.
KW - brain slice
KW - calcium
KW - cAMP
KW - caudate nucleus
KW - neurotensin
KW - protein phosphorylation
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UR - http://www.scopus.com/inward/citedby.url?scp=0026007791&partnerID=8YFLogxK
U2 - 10.1016/0278-5846(91)90043-Z
DO - 10.1016/0278-5846(91)90043-Z
M3 - Article
VL - 15
SP - 83
EP - 89
JO - Progress in Neuro-Psychopharmacology and Biological Psychiatry
JF - Progress in Neuro-Psychopharmacology and Biological Psychiatry
SN - 0278-5846
IS - 1
ER -