Neurotensin effects on calcium/calmodulin-dependent protein phosphorylation in rat neostriatal slices

John Kasckow, Scott T. Cain, Charles Nemeroff

Research output: Contribution to journalArticle

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Abstract

Neurotensin (NT) is an endogenous brain tridecapeptide for which high affinity binding sites exist in the central nervous system. We have investigated the effects of NT incubation with rat neotriatal slices on calcium/calmodulin (Ca/CaM)-dependent protein phosphorylation. Slices were incubated with NT (5 or 50 nM) for 3, 10, 16, or 30 min followed by in vitro phosphorylation, electrophoresis and autoradiography. NT significantly altered the phosphorylation of a 62 kDa protein which is likely the β subunit of the Ca/CaM dependent protein kinase. These changes may reflect the ability of NT to influence calcium mediated signal transduction.

Original languageEnglish
Pages (from-to)343-346
Number of pages4
JournalBrain Research
Volume545
Issue number1-2
DOIs
StatePublished - Apr 5 1991
Externally publishedYes

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Keywords

  • Brain slice
  • Calcium
  • Calmodulin
  • Neostriatum
  • Neurotensin
  • Protein phosphorylation

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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