Neuropeptide Y (NPY) in the area of the hypothalamic paraventricular nucleus activates the pituitary-adrenocortical axis in the rat

Claes R Wahlestedt, Gunnar Skagerberg, Rolf Ekman, Markus Heilig, Frank Sundler, Rolf Håkanson

Research output: Contribution to journalArticle

274 Citations (Scopus)

Abstract

Immunocytochemical studies have documented the presence of neuropeptide Y (NPY) in the hypothalamic paraventricular nucleus (PVN) which harbours a large number of neurones that contain corticotrophin-releasing factor (CRF). In this study the close morphological association between NPY fibres and CRF cell bodies in the PVN was confirmed. The localization of NPY terminals in the vicinity of CRF neurones forms a morphological basis for an action of NPY in the hypothalamic control of the pituitary-adrenocortical axis. We therefore microinjected NPY into the area of the PVN of both conscious, freely moving and anaesthetized rats and noted a powerful stimulatory effect on adrenocorticotropic hormone (ACTH) and corticosterone release as measured by radioimmunoassay. In experiments with conscious, freely moving rats, higher ACTH and corticosterone levels were detected following injection of NPY into the area of the PVN than following control injection (desamidated NPY). Intracerebroventricular injection of NPY produced a small, albeit significant, increase in circulating corticosterone levels as compared to control (saline-injected) rats. Anaesthetized rats responded to NPY (but not to saline) injected into the area of the PVN with elevated ACTH and corticosterone levels, while injection of NPY into the neocortex failed to affect the blood concentration of either ACTH or corticosterone. In conclusion, we have demonstrated an activating effect of NPY on the pituitary-adrenocortical axis both in conscious and anaesthetized rats which may reflect the anatomical relationship between NPY fibres and CRF neurones in the PVN.

Original languageEnglish
Pages (from-to)33-38
Number of pages6
JournalBrain Research
Volume417
Issue number1
DOIs
StatePublished - Aug 4 1987
Externally publishedYes

Fingerprint

Paraventricular Hypothalamic Nucleus
Neuropeptide Y
Corticosterone
Corticotropin-Releasing Hormone
Adrenocorticotropic Hormone
Injections
Neurons
Neocortex
Radioimmunoassay

Keywords

  • Adrenocorticotropic hormone (ACTH)
  • Corticosterone
  • Desamido-NPY
  • Hypothalamus
  • Neuropeptide Y
  • Paraventricular nucleus

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Neuropeptide Y (NPY) in the area of the hypothalamic paraventricular nucleus activates the pituitary-adrenocortical axis in the rat. / Wahlestedt, Claes R; Skagerberg, Gunnar; Ekman, Rolf; Heilig, Markus; Sundler, Frank; Håkanson, Rolf.

In: Brain Research, Vol. 417, No. 1, 04.08.1987, p. 33-38.

Research output: Contribution to journalArticle

Wahlestedt, Claes R ; Skagerberg, Gunnar ; Ekman, Rolf ; Heilig, Markus ; Sundler, Frank ; Håkanson, Rolf. / Neuropeptide Y (NPY) in the area of the hypothalamic paraventricular nucleus activates the pituitary-adrenocortical axis in the rat. In: Brain Research. 1987 ; Vol. 417, No. 1. pp. 33-38.
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