TY - JOUR
T1 - Neurons and glia in purified retinal cultures identified by monoclonal antibodies to intermediate filaments
AU - Hyndman, Arnold G.
AU - Lemmon, Vance
N1 - Funding Information:
This work was supported by a grant from the Charles and Johanna Busch Memorial Fund, a grant from the National Science Foundation PRM 82-12393 to A.G.H., and grants from the March of Dimes (5-395) and the National Eye Institute (EY05285) to V.L. The antibody RT-97-F1 was a generous gift of Dr. H.M. Geller, Robert Wood Johnson Medical School. Authors wish to thank Dr. Gail Zeevalk for her technical assistance and critical reading of the manuscript.
PY - 1987/3/31
Y1 - 1987/3/31
N2 - Two monoclonal antibodies known to bind intermediate filaments were used to identify neurons and glia from embryonic chick neural retina. Neurofilament specific antibody RT-97-F1, bound neuroepithelial cells, migrating neurons, as well as the photoreceptor layer, plexiform layers and optic fiber layer. The other, 3A7, bound intermediate filaments of Müller cells. In purified neuronal cultures, RT-97-F1 bound 75, 83 and 98% of the cells present at 24, 48 and 72 h respectively, while 3A7 bound 26, 15 and 0.3% for the same times in vitro. In purified glial cultures, RT-97-F1 showed a weak perinuclear binding, while 3A7 strongly bound intermediate filaments of nearly 100% of the cells. These antibodies confirmed and quantitated the high purity of our cultures.
AB - Two monoclonal antibodies known to bind intermediate filaments were used to identify neurons and glia from embryonic chick neural retina. Neurofilament specific antibody RT-97-F1, bound neuroepithelial cells, migrating neurons, as well as the photoreceptor layer, plexiform layers and optic fiber layer. The other, 3A7, bound intermediate filaments of Müller cells. In purified neuronal cultures, RT-97-F1 bound 75, 83 and 98% of the cells present at 24, 48 and 72 h respectively, while 3A7 bound 26, 15 and 0.3% for the same times in vitro. In purified glial cultures, RT-97-F1 showed a weak perinuclear binding, while 3A7 strongly bound intermediate filaments of nearly 100% of the cells. These antibodies confirmed and quantitated the high purity of our cultures.
KW - Culture
KW - Glia
KW - Intermediate filament
KW - Monoclonal antibody
KW - Neuron
KW - Retina
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U2 - 10.1016/0304-3940(87)90284-9
DO - 10.1016/0304-3940(87)90284-9
M3 - Article
C2 - 3554012
AN - SCOPUS:0023091901
VL - 75
SP - 121
EP - 126
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 2
ER -