Neurones express high levels of a structurally modified, activated form of pp60c-src

Joan S. Brugge, Patricia C. Cotton, A. E. Queral, John N. Barrett, Doris Nonner, Robert W. Keane

Research output: Contribution to journalArticle

228 Scopus citations

Abstract

Neural tissues contain high levels of the cellular homologue of the transforming protein of Rous sarcoma virus (RSV), but neither the specific cell types expressing high levels of c-src, nor the function of the cellular src (c-src) protein has been determined. Using primary culture methods, we have found that pure neurones and astrocytes derived from the rat central nervous system (CNS) contain 15- to 20-times higher levels of the c-src protein than fibroblasts. However, the specific activity of the c-src protein from the neuronal cultures is 6- to 12-times higher than that from the astrocyte cultures. In addition, the c-src protein expressed in neuronal cultures contains a structural alteration within the amino-terminal region of the molecule that causes a shift in the mobility of the c-src protein on the SDS-polyacrylamide gels. These results indicate that a structurally distinct form of the cellular src protein that possesses an activated tyrosylkinase activity is expressed at very high levels in post-mitotic CNS neurones.

Original languageEnglish (US)
Pages (from-to)554-557
Number of pages4
JournalNature
Volume316
Issue number6028
DOIs
StatePublished - Dec 1 1985

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