Abstract
Neural tissues contain high levels of the cellular homologue of the transforming protein of Rous sarcoma virus (RSV), but neither the specific cell types expressing high levels of c-src, nor the function of the cellular src (c-src) protein has been determined. Using primary culture methods, we have found that pure neurones and astrocytes derived from the rat central nervous system (CNS) contain 15- to 20-times higher levels of the c-src protein than fibroblasts. However, the specific activity of the c-src protein from the neuronal cultures is 6- to 12-times higher than that from the astrocyte cultures. In addition, the c-src protein expressed in neuronal cultures contains a structural alteration within the amino-terminal region of the molecule that causes a shift in the mobility of the c-src protein on the SDS-polyacrylamide gels. These results indicate that a structurally distinct form of the cellular src protein that possesses an activated tyrosylkinase activity is expressed at very high levels in post-mitotic CNS neurones.
| Original language | English |
|---|---|
| Pages (from-to) | 554-557 |
| Number of pages | 4 |
| Journal | Nature |
| Volume | 316 |
| Issue number | 6028 |
| DOIs | |
| State | Published - Dec 1 1985 |
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Neurones express high levels of a structurally modified, activated form of pp60(c-src). / Brugge, J. S.; Cotton, P. C.; Queral, A. E.; Barrett, John; Nonner, D.; Keane, Robert.
In: Nature, Vol. 316, No. 6028, 01.12.1985, p. 554-557.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neurones express high levels of a structurally modified, activated form of pp60(c-src)
AU - Brugge, J. S.
AU - Cotton, P. C.
AU - Queral, A. E.
AU - Barrett, John
AU - Nonner, D.
AU - Keane, Robert
PY - 1985/12/1
Y1 - 1985/12/1
N2 - Neural tissues contain high levels of the cellular homologue of the transforming protein of Rous sarcoma virus (RSV), but neither the specific cell types expressing high levels of c-src, nor the function of the cellular src (c-src) protein has been determined. Using primary culture methods, we have found that pure neurones and astrocytes derived from the rat central nervous system (CNS) contain 15- to 20-times higher levels of the c-src protein than fibroblasts. However, the specific activity of the c-src protein from the neuronal cultures is 6- to 12-times higher than that from the astrocyte cultures. In addition, the c-src protein expressed in neuronal cultures contains a structural alteration within the amino-terminal region of the molecule that causes a shift in the mobility of the c-src protein on the SDS-polyacrylamide gels. These results indicate that a structurally distinct form of the cellular src protein that possesses an activated tyrosylkinase activity is expressed at very high levels in post-mitotic CNS neurones.
AB - Neural tissues contain high levels of the cellular homologue of the transforming protein of Rous sarcoma virus (RSV), but neither the specific cell types expressing high levels of c-src, nor the function of the cellular src (c-src) protein has been determined. Using primary culture methods, we have found that pure neurones and astrocytes derived from the rat central nervous system (CNS) contain 15- to 20-times higher levels of the c-src protein than fibroblasts. However, the specific activity of the c-src protein from the neuronal cultures is 6- to 12-times higher than that from the astrocyte cultures. In addition, the c-src protein expressed in neuronal cultures contains a structural alteration within the amino-terminal region of the molecule that causes a shift in the mobility of the c-src protein on the SDS-polyacrylamide gels. These results indicate that a structurally distinct form of the cellular src protein that possesses an activated tyrosylkinase activity is expressed at very high levels in post-mitotic CNS neurones.
UR - http://www.scopus.com/inward/record.url?scp=0022372469&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022372469&partnerID=8YFLogxK
U2 - 10.1038/316554a0
DO - 10.1038/316554a0
M3 - Article
C2 - 2412121
AN - SCOPUS:0022372469
VL - 316
SP - 554
EP - 557
JO - Nature
JF - Nature
SN - 0028-0836
IS - 6028
ER -