Abstract
Leukocytes play an important role in the development of ischemia/reperfusion injury. Recent work in our laboratory has demonstrated that a mixture of synthetic fibronectin peptides to leukocyte adhesion molecules reduces ischemic brain damage after transient focal cerebral ischemia. The purpose of this study was to evaluate the efficacy of the individual peptides on leukocyte accumulation, infarct size, and neurological outcome in rats subjected to 1 h of cerebral ischemia and 48 h of reperfusion. Thirty-five animals were divided into five groups: transient ischemia without treatment (Group I), treatment with arginyl-glycyl-aspartic acid (RGD) peptide (Group II), connecting segment (CS)-1 peptide (Group III), fibronectin (FN)-C/H-V peptide (Group IV), and scrambled FN-C/H-V peptide (Group V). Groups III and IV showed a significant decrease in the degree of leukocyte infiltration in the lesion and in the infarct size (p < 0.05) when compared to Groups I, II, and V. The neurological grade of Groups III and IV was significantly better than in Groups I, II, and V at 48 h after reperfusion (p < 0.01). Thus, in addition to demonstrating the potential efficacy of synthetic peptides as therapeutic agents for ischemia reperfusion, these results also offer new insights into the mechanisms of leukocyte arrest and recruitment in ischemia/reperfusion injury.
| Original language | English |
|---|---|
| Pages (from-to) | 1120-1125 |
| Number of pages | 6 |
| Journal | Journal of Cerebral Blood Flow and Metabolism |
| Volume | 16 |
| Issue number | 6 |
| State | Published - Nov 12 1996 |
| Externally published | Yes |
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Keywords
- Adhesion molecules
- Cerebral ischemia
- Fibronectin
- Leukocytes
- Rats
- Reperfusion
ASJC Scopus subject areas
- Endocrinology
- Neuroscience(all)
- Endocrinology, Diabetes and Metabolism
Cite this
Neuronal protection from cerebral ischemia by synthetic fibronectin peptides to leukocyte adhesion molecules. / Yanaka, Kiyoyuki; Camarata, Paul J.; Spellman, Stephen R.; McCarthy, James B.; Furcht, Leo T.; Low, Walter C.; Heros, Roberto.
In: Journal of Cerebral Blood Flow and Metabolism, Vol. 16, No. 6, 12.11.1996, p. 1120-1125.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neuronal protection from cerebral ischemia by synthetic fibronectin peptides to leukocyte adhesion molecules
AU - Yanaka, Kiyoyuki
AU - Camarata, Paul J.
AU - Spellman, Stephen R.
AU - McCarthy, James B.
AU - Furcht, Leo T.
AU - Low, Walter C.
AU - Heros, Roberto
PY - 1996/11/12
Y1 - 1996/11/12
N2 - Leukocytes play an important role in the development of ischemia/reperfusion injury. Recent work in our laboratory has demonstrated that a mixture of synthetic fibronectin peptides to leukocyte adhesion molecules reduces ischemic brain damage after transient focal cerebral ischemia. The purpose of this study was to evaluate the efficacy of the individual peptides on leukocyte accumulation, infarct size, and neurological outcome in rats subjected to 1 h of cerebral ischemia and 48 h of reperfusion. Thirty-five animals were divided into five groups: transient ischemia without treatment (Group I), treatment with arginyl-glycyl-aspartic acid (RGD) peptide (Group II), connecting segment (CS)-1 peptide (Group III), fibronectin (FN)-C/H-V peptide (Group IV), and scrambled FN-C/H-V peptide (Group V). Groups III and IV showed a significant decrease in the degree of leukocyte infiltration in the lesion and in the infarct size (p < 0.05) when compared to Groups I, II, and V. The neurological grade of Groups III and IV was significantly better than in Groups I, II, and V at 48 h after reperfusion (p < 0.01). Thus, in addition to demonstrating the potential efficacy of synthetic peptides as therapeutic agents for ischemia reperfusion, these results also offer new insights into the mechanisms of leukocyte arrest and recruitment in ischemia/reperfusion injury.
AB - Leukocytes play an important role in the development of ischemia/reperfusion injury. Recent work in our laboratory has demonstrated that a mixture of synthetic fibronectin peptides to leukocyte adhesion molecules reduces ischemic brain damage after transient focal cerebral ischemia. The purpose of this study was to evaluate the efficacy of the individual peptides on leukocyte accumulation, infarct size, and neurological outcome in rats subjected to 1 h of cerebral ischemia and 48 h of reperfusion. Thirty-five animals were divided into five groups: transient ischemia without treatment (Group I), treatment with arginyl-glycyl-aspartic acid (RGD) peptide (Group II), connecting segment (CS)-1 peptide (Group III), fibronectin (FN)-C/H-V peptide (Group IV), and scrambled FN-C/H-V peptide (Group V). Groups III and IV showed a significant decrease in the degree of leukocyte infiltration in the lesion and in the infarct size (p < 0.05) when compared to Groups I, II, and V. The neurological grade of Groups III and IV was significantly better than in Groups I, II, and V at 48 h after reperfusion (p < 0.01). Thus, in addition to demonstrating the potential efficacy of synthetic peptides as therapeutic agents for ischemia reperfusion, these results also offer new insights into the mechanisms of leukocyte arrest and recruitment in ischemia/reperfusion injury.
KW - Adhesion molecules
KW - Cerebral ischemia
KW - Fibronectin
KW - Leukocytes
KW - Rats
KW - Reperfusion
UR - http://www.scopus.com/inward/record.url?scp=0029962665&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029962665&partnerID=8YFLogxK
M3 - Article
C2 - 8898683
AN - SCOPUS:0029962665
VL - 16
SP - 1120
EP - 1125
JO - Journal of Cerebral Blood Flow and Metabolism
JF - Journal of Cerebral Blood Flow and Metabolism
SN - 0271-678X
IS - 6
ER -