Neuronal protection from cerebral ischemia by synthetic fibronectin peptides to leukocyte adhesion molecules

Kiyoyuki Yanaka, Paul J. Camarata, Stephen R. Spellman, James B. McCarthy, Leo T. Furcht, Walter C. Low, Roberto Heros

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Leukocytes play an important role in the development of ischemia/reperfusion injury. Recent work in our laboratory has demonstrated that a mixture of synthetic fibronectin peptides to leukocyte adhesion molecules reduces ischemic brain damage after transient focal cerebral ischemia. The purpose of this study was to evaluate the efficacy of the individual peptides on leukocyte accumulation, infarct size, and neurological outcome in rats subjected to 1 h of cerebral ischemia and 48 h of reperfusion. Thirty-five animals were divided into five groups: transient ischemia without treatment (Group I), treatment with arginyl-glycyl-aspartic acid (RGD) peptide (Group II), connecting segment (CS)-1 peptide (Group III), fibronectin (FN)-C/H-V peptide (Group IV), and scrambled FN-C/H-V peptide (Group V). Groups III and IV showed a significant decrease in the degree of leukocyte infiltration in the lesion and in the infarct size (p < 0.05) when compared to Groups I, II, and V. The neurological grade of Groups III and IV was significantly better than in Groups I, II, and V at 48 h after reperfusion (p < 0.01). Thus, in addition to demonstrating the potential efficacy of synthetic peptides as therapeutic agents for ischemia reperfusion, these results also offer new insights into the mechanisms of leukocyte arrest and recruitment in ischemia/reperfusion injury.

Original languageEnglish
Pages (from-to)1120-1125
Number of pages6
JournalJournal of Cerebral Blood Flow and Metabolism
Volume16
Issue number6
StatePublished - Nov 12 1996
Externally publishedYes

Fingerprint

Cell Adhesion Molecules
Brain Ischemia
Fibronectins
Leukocytes
Reperfusion
Peptides
Reperfusion Injury
Ischemia
Transient Ischemic Attack
Neuroprotection
Brain
peptide V
arginyl-glycyl-aspartic acid
Therapeutics

Keywords

  • Adhesion molecules
  • Cerebral ischemia
  • Fibronectin
  • Leukocytes
  • Rats
  • Reperfusion

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Yanaka, K., Camarata, P. J., Spellman, S. R., McCarthy, J. B., Furcht, L. T., Low, W. C., & Heros, R. (1996). Neuronal protection from cerebral ischemia by synthetic fibronectin peptides to leukocyte adhesion molecules. Journal of Cerebral Blood Flow and Metabolism, 16(6), 1120-1125.

Neuronal protection from cerebral ischemia by synthetic fibronectin peptides to leukocyte adhesion molecules. / Yanaka, Kiyoyuki; Camarata, Paul J.; Spellman, Stephen R.; McCarthy, James B.; Furcht, Leo T.; Low, Walter C.; Heros, Roberto.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 16, No. 6, 12.11.1996, p. 1120-1125.

Research output: Contribution to journalArticle

Yanaka, K, Camarata, PJ, Spellman, SR, McCarthy, JB, Furcht, LT, Low, WC & Heros, R 1996, 'Neuronal protection from cerebral ischemia by synthetic fibronectin peptides to leukocyte adhesion molecules', Journal of Cerebral Blood Flow and Metabolism, vol. 16, no. 6, pp. 1120-1125.
Yanaka K, Camarata PJ, Spellman SR, McCarthy JB, Furcht LT, Low WC et al. Neuronal protection from cerebral ischemia by synthetic fibronectin peptides to leukocyte adhesion molecules. Journal of Cerebral Blood Flow and Metabolism. 1996 Nov 12;16(6):1120-1125.
Yanaka, Kiyoyuki ; Camarata, Paul J. ; Spellman, Stephen R. ; McCarthy, James B. ; Furcht, Leo T. ; Low, Walter C. ; Heros, Roberto. / Neuronal protection from cerebral ischemia by synthetic fibronectin peptides to leukocyte adhesion molecules. In: Journal of Cerebral Blood Flow and Metabolism. 1996 ; Vol. 16, No. 6. pp. 1120-1125.
@article{e74229ce0ff546a2ac538fce02967ef1,
title = "Neuronal protection from cerebral ischemia by synthetic fibronectin peptides to leukocyte adhesion molecules",
abstract = "Leukocytes play an important role in the development of ischemia/reperfusion injury. Recent work in our laboratory has demonstrated that a mixture of synthetic fibronectin peptides to leukocyte adhesion molecules reduces ischemic brain damage after transient focal cerebral ischemia. The purpose of this study was to evaluate the efficacy of the individual peptides on leukocyte accumulation, infarct size, and neurological outcome in rats subjected to 1 h of cerebral ischemia and 48 h of reperfusion. Thirty-five animals were divided into five groups: transient ischemia without treatment (Group I), treatment with arginyl-glycyl-aspartic acid (RGD) peptide (Group II), connecting segment (CS)-1 peptide (Group III), fibronectin (FN)-C/H-V peptide (Group IV), and scrambled FN-C/H-V peptide (Group V). Groups III and IV showed a significant decrease in the degree of leukocyte infiltration in the lesion and in the infarct size (p < 0.05) when compared to Groups I, II, and V. The neurological grade of Groups III and IV was significantly better than in Groups I, II, and V at 48 h after reperfusion (p < 0.01). Thus, in addition to demonstrating the potential efficacy of synthetic peptides as therapeutic agents for ischemia reperfusion, these results also offer new insights into the mechanisms of leukocyte arrest and recruitment in ischemia/reperfusion injury.",
keywords = "Adhesion molecules, Cerebral ischemia, Fibronectin, Leukocytes, Rats, Reperfusion",
author = "Kiyoyuki Yanaka and Camarata, {Paul J.} and Spellman, {Stephen R.} and McCarthy, {James B.} and Furcht, {Leo T.} and Low, {Walter C.} and Roberto Heros",
year = "1996",
month = "11",
day = "12",
language = "English",
volume = "16",
pages = "1120--1125",
journal = "Journal of Cerebral Blood Flow and Metabolism",
issn = "0271-678X",
publisher = "Nature Publishing Group",
number = "6",

}

TY - JOUR

T1 - Neuronal protection from cerebral ischemia by synthetic fibronectin peptides to leukocyte adhesion molecules

AU - Yanaka, Kiyoyuki

AU - Camarata, Paul J.

AU - Spellman, Stephen R.

AU - McCarthy, James B.

AU - Furcht, Leo T.

AU - Low, Walter C.

AU - Heros, Roberto

PY - 1996/11/12

Y1 - 1996/11/12

N2 - Leukocytes play an important role in the development of ischemia/reperfusion injury. Recent work in our laboratory has demonstrated that a mixture of synthetic fibronectin peptides to leukocyte adhesion molecules reduces ischemic brain damage after transient focal cerebral ischemia. The purpose of this study was to evaluate the efficacy of the individual peptides on leukocyte accumulation, infarct size, and neurological outcome in rats subjected to 1 h of cerebral ischemia and 48 h of reperfusion. Thirty-five animals were divided into five groups: transient ischemia without treatment (Group I), treatment with arginyl-glycyl-aspartic acid (RGD) peptide (Group II), connecting segment (CS)-1 peptide (Group III), fibronectin (FN)-C/H-V peptide (Group IV), and scrambled FN-C/H-V peptide (Group V). Groups III and IV showed a significant decrease in the degree of leukocyte infiltration in the lesion and in the infarct size (p < 0.05) when compared to Groups I, II, and V. The neurological grade of Groups III and IV was significantly better than in Groups I, II, and V at 48 h after reperfusion (p < 0.01). Thus, in addition to demonstrating the potential efficacy of synthetic peptides as therapeutic agents for ischemia reperfusion, these results also offer new insights into the mechanisms of leukocyte arrest and recruitment in ischemia/reperfusion injury.

AB - Leukocytes play an important role in the development of ischemia/reperfusion injury. Recent work in our laboratory has demonstrated that a mixture of synthetic fibronectin peptides to leukocyte adhesion molecules reduces ischemic brain damage after transient focal cerebral ischemia. The purpose of this study was to evaluate the efficacy of the individual peptides on leukocyte accumulation, infarct size, and neurological outcome in rats subjected to 1 h of cerebral ischemia and 48 h of reperfusion. Thirty-five animals were divided into five groups: transient ischemia without treatment (Group I), treatment with arginyl-glycyl-aspartic acid (RGD) peptide (Group II), connecting segment (CS)-1 peptide (Group III), fibronectin (FN)-C/H-V peptide (Group IV), and scrambled FN-C/H-V peptide (Group V). Groups III and IV showed a significant decrease in the degree of leukocyte infiltration in the lesion and in the infarct size (p < 0.05) when compared to Groups I, II, and V. The neurological grade of Groups III and IV was significantly better than in Groups I, II, and V at 48 h after reperfusion (p < 0.01). Thus, in addition to demonstrating the potential efficacy of synthetic peptides as therapeutic agents for ischemia reperfusion, these results also offer new insights into the mechanisms of leukocyte arrest and recruitment in ischemia/reperfusion injury.

KW - Adhesion molecules

KW - Cerebral ischemia

KW - Fibronectin

KW - Leukocytes

KW - Rats

KW - Reperfusion

UR - http://www.scopus.com/inward/record.url?scp=0029962665&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029962665&partnerID=8YFLogxK

M3 - Article

C2 - 8898683

AN - SCOPUS:0029962665

VL - 16

SP - 1120

EP - 1125

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

SN - 0271-678X

IS - 6

ER -