Neuronal pentraxin with chromo domain (NPCD) is a novel class of protein expressed in multiple neuronal domains

Bo Chen, John L. Bixby

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The receptor tyrosine phosphatase PTPRO is involved in axon guidance, but its intracellular signaling mechanisms are unknown. Signals generated through PTPRO must involve interaction of the intracellular domain with substrates and/or signaling proteins. By screening for proteins interacting with PTPRO's intracellular domain, we have identified a new class of cytoplasmic protein. This novel protein, NPCD (Neuronal Pentraxin with Chromo Domain), has multiple cytoplasmic isoforms generated by alternative splicing that are selectively expressed in neurons. These cytoplasmic NPCD isoforms are composed of a neuronal pentraxin domain (formerly thought exclusively extracellular) linked to a chromo domain (formerly thought exclusively nuclear); this protein motif organization is unprecedented. NPCD isoforms are expressed in numerous regions of the central nervous system, where they are present in distinct subcellular arrangements in different brain regions. NPCD isoforms are mainly associated with the inner side of the plasma membrane in brain neurons and rat PC12 cells in vitro; they are present in cell bodies, processes, and growth cones. The biochemical complexity and neuronal expression pattern of NPCD, together with its interaction with PTPRO, suggests involvement in multiple neuronal processes.

Original languageEnglish (US)
Pages (from-to)391-402
Number of pages12
JournalJournal of Comparative Neurology
Volume481
Issue number4
DOIs
StatePublished - Jan 24 2005

Keywords

  • Axon growth
  • Chromobox homolog
  • PC12 cell
  • Tyrosine phosphatase

ASJC Scopus subject areas

  • Neuroscience(all)

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