Neuronal nicotinic acetylcholine receptor expression by O2A/Oligodendrocyte progenitor cells

Scott W. Rogers, Ninel Gregori, Noel Carlson, Lorise C. Gahring, Mark Noble

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Oligodendrocyte precursor cells (O2A/OPC, A2B5+) were examined for expression of neuronal nicotinic acetylcholine receptors (nAChR). RT-PCR analysis and immunocytochemistry of O2A/OPCs purified from the rat corpus collusum revealed the expression of nAChR subunits (α3, α4, α5, α7, β2, and β4. Immunoreactivity toward nAChR subunits was not detected in cells induced to differentiate into either oligodendrocytes or astrocytes. Approximately 65% of O2A/OPCs loaded with the calcium-responsive dye FURA-2 increased their intracellular free calcium in response to nicotine application. This response was sensitive to the nAChRα4/β2 antagonist, dihydro-β-erythroidine (DHβE), and the voltage-gated calcium channel antagonist, nifedipine. A subset of nicotine-responsive cells (37%) established DHβE or nifedipine-sensitive intracellular free calcium oscillations that continued in the presence of nicotine. Typical oscillations occurred at intervals of 20 to 30 s with progressively diminished amplitudes over a period of 2 to 3 min. In rare cases, oscillations persisted for as long as 10 min. O2A/OPCs exposed to carbachol or AMPA produced no oscillations despite robust increases in intracellular free calcium. The expression of nAChRs in non-neuronal glial precursor cells suggests an expanded role for this receptor system in the development of the mammalian brain.

Original languageEnglish
Pages (from-to)306-313
Number of pages8
JournalGLIA
Volume33
Issue number4
DOIs
StatePublished - Apr 23 2001
Externally publishedYes

Fingerprint

Oligodendroglia
Nicotinic Receptors
Nicotine
Stem Cells
Nifedipine
Calcium
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Calcium Signaling
Calcium Channel Blockers
Carbachol
Neuroglia
Astrocytes
Immunohistochemistry
Polymerase Chain Reaction
Brain

Keywords

  • Brain
  • Culture
  • Development
  • Nicotine
  • O2A/OPC
  • Precursor
  • Receptor

ASJC Scopus subject areas

  • Immunology

Cite this

Neuronal nicotinic acetylcholine receptor expression by O2A/Oligodendrocyte progenitor cells. / Rogers, Scott W.; Gregori, Ninel; Carlson, Noel; Gahring, Lorise C.; Noble, Mark.

In: GLIA, Vol. 33, No. 4, 23.04.2001, p. 306-313.

Research output: Contribution to journalArticle

Rogers, Scott W. ; Gregori, Ninel ; Carlson, Noel ; Gahring, Lorise C. ; Noble, Mark. / Neuronal nicotinic acetylcholine receptor expression by O2A/Oligodendrocyte progenitor cells. In: GLIA. 2001 ; Vol. 33, No. 4. pp. 306-313.
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