Neurohypophysial-hormone-responsive cell line derived from a dimethylbenzanthracene-induced rat mammary tumour

M. E. Monaco, W. R. Kidwell, M. E. Lippman

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


WRK-1, a cloned cell line derived from a rat mammary tumour, responds to physiological concentrations of vasopressin and pharmacological concentrations of oxytocin with increased incorporation of [14C]acetate into lipids and increased protein accumulation. The presence of pharmacological concentrations of insulin, which itself is active on the WRK-1 cells, further enhances the effects of the neurohypophysial hormones. Unlike the action of vasopressin on other responsive tissues, the stimulation of acetate incorporation by WRK-1 cells is not observed until 24 h after the addition of the hormone. The lipids synthesized in response to the hormones are predominantly polar lipids, rather than the triacylglycerols characteristic of the differentiated mammary gland. [1-Deaminocysteine, 8-D-arginine]vasopressin, a vasopressin analogue that lacks pressor activity, has no effect on WRK-1 cells.

Original languageEnglish (US)
Pages (from-to)437-441
Number of pages5
JournalBiochemical Journal
Issue number2
StatePublished - 1980
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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