Abstract
WRK-1, a cloned cell line derived from a rat mammary tumour, responds to physiological concentrations of vasopressin and pharmacological concentrations of oxytocin with increased incorporation of [14C]acetate into lipids and increased protein accumulation. The presence of pharmacological concentrations of insulin, which itself is active on the WRK-1 cells, further enhances the effects of the neurohypophysial hormones. Unlike the action of vasopressin on other responsive tissues, the stimulation of acetate incorporation by WRK-1 cells is not observed until 24 h after the addition of the hormone. The lipids synthesized in response to the hormones are predominantly polar lipids, rather than the triacylglycerols characteristic of the differentiated mammary gland. [1-Deaminocysteine, 8-D-arginine]vasopressin, a vasopressin analogue that lacks pressor activity, has no effect on WRK-1 cells.
Original language | English (US) |
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Pages (from-to) | 437-441 |
Number of pages | 5 |
Journal | Biochemical Journal |
Volume | 188 |
Issue number | 2 |
DOIs | |
State | Published - 1980 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology