Neurochemical consequences of status epilepticus induced in rats by coadministration of lithium and pilocarpine

Richard S Jope, Richard A. Morrisett

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Status epilepticus was produced in rats by administering pilocarpine (30 mg/kg, s.c.) 16 h after treatment with LiCl (3 meq/kg, i.p.). After 35 min of status epilepticus, several parameters of cholinergic activity were measured. Seizures had no effect on the in vivo concentration of acetylcholine or choline in cerebellum, cortex, hippocampus, or striatum. Synaptosomal high-affinity choline transport was also not changed by seizures in hippocampus, cortex, or striatum. Cortical slices from seizing rats had elevated concentrations of acetylcholine and released acetylcholine at a greater rate than did controls, but these effects seemed to be due to a reduction in the postmortem hydrolysis of acetylcholine. Synaptosomal 45calcium uptake during 2 to 60 s of incubation was no different from control rates in tissue prepared from seizing rats. These results indicate that presynaptic cholinergic activity is not markedly altered by 35 min of continuous seizure activity induced by lithium and pilocarpine. In contrast, the in vivo concentration of cyclic guanosine 5′-monophosphate was elevated above control values in seizing rats by 57 to 170% in cerebellum, cortex, hippocampus, and striatum.

Original languageEnglish
Pages (from-to)404-414
Number of pages11
JournalExperimental Neurology
Volume93
Issue number2
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Pilocarpine
Status Epilepticus
Lithium
Acetylcholine
Hippocampus
Seizures
Choline
Cerebellum
Cholinergic Agents
Guanosine Monophosphate
Hydrolysis

ASJC Scopus subject areas

  • Neuroscience(all)
  • Neurology

Cite this

Neurochemical consequences of status epilepticus induced in rats by coadministration of lithium and pilocarpine. / Jope, Richard S; Morrisett, Richard A.

In: Experimental Neurology, Vol. 93, No. 2, 01.01.1986, p. 404-414.

Research output: Contribution to journalArticle

@article{41d1fb9010f0407f97b7443f9735c05f,
title = "Neurochemical consequences of status epilepticus induced in rats by coadministration of lithium and pilocarpine",
abstract = "Status epilepticus was produced in rats by administering pilocarpine (30 mg/kg, s.c.) 16 h after treatment with LiCl (3 meq/kg, i.p.). After 35 min of status epilepticus, several parameters of cholinergic activity were measured. Seizures had no effect on the in vivo concentration of acetylcholine or choline in cerebellum, cortex, hippocampus, or striatum. Synaptosomal high-affinity choline transport was also not changed by seizures in hippocampus, cortex, or striatum. Cortical slices from seizing rats had elevated concentrations of acetylcholine and released acetylcholine at a greater rate than did controls, but these effects seemed to be due to a reduction in the postmortem hydrolysis of acetylcholine. Synaptosomal 45calcium uptake during 2 to 60 s of incubation was no different from control rates in tissue prepared from seizing rats. These results indicate that presynaptic cholinergic activity is not markedly altered by 35 min of continuous seizure activity induced by lithium and pilocarpine. In contrast, the in vivo concentration of cyclic guanosine 5′-monophosphate was elevated above control values in seizing rats by 57 to 170{\%} in cerebellum, cortex, hippocampus, and striatum.",
author = "Jope, {Richard S} and Morrisett, {Richard A.}",
year = "1986",
month = "1",
day = "1",
doi = "10.1016/0014-4886(86)90200-1",
language = "English",
volume = "93",
pages = "404--414",
journal = "Experimental Neurology",
issn = "0014-4886",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Neurochemical consequences of status epilepticus induced in rats by coadministration of lithium and pilocarpine

AU - Jope, Richard S

AU - Morrisett, Richard A.

PY - 1986/1/1

Y1 - 1986/1/1

N2 - Status epilepticus was produced in rats by administering pilocarpine (30 mg/kg, s.c.) 16 h after treatment with LiCl (3 meq/kg, i.p.). After 35 min of status epilepticus, several parameters of cholinergic activity were measured. Seizures had no effect on the in vivo concentration of acetylcholine or choline in cerebellum, cortex, hippocampus, or striatum. Synaptosomal high-affinity choline transport was also not changed by seizures in hippocampus, cortex, or striatum. Cortical slices from seizing rats had elevated concentrations of acetylcholine and released acetylcholine at a greater rate than did controls, but these effects seemed to be due to a reduction in the postmortem hydrolysis of acetylcholine. Synaptosomal 45calcium uptake during 2 to 60 s of incubation was no different from control rates in tissue prepared from seizing rats. These results indicate that presynaptic cholinergic activity is not markedly altered by 35 min of continuous seizure activity induced by lithium and pilocarpine. In contrast, the in vivo concentration of cyclic guanosine 5′-monophosphate was elevated above control values in seizing rats by 57 to 170% in cerebellum, cortex, hippocampus, and striatum.

AB - Status epilepticus was produced in rats by administering pilocarpine (30 mg/kg, s.c.) 16 h after treatment with LiCl (3 meq/kg, i.p.). After 35 min of status epilepticus, several parameters of cholinergic activity were measured. Seizures had no effect on the in vivo concentration of acetylcholine or choline in cerebellum, cortex, hippocampus, or striatum. Synaptosomal high-affinity choline transport was also not changed by seizures in hippocampus, cortex, or striatum. Cortical slices from seizing rats had elevated concentrations of acetylcholine and released acetylcholine at a greater rate than did controls, but these effects seemed to be due to a reduction in the postmortem hydrolysis of acetylcholine. Synaptosomal 45calcium uptake during 2 to 60 s of incubation was no different from control rates in tissue prepared from seizing rats. These results indicate that presynaptic cholinergic activity is not markedly altered by 35 min of continuous seizure activity induced by lithium and pilocarpine. In contrast, the in vivo concentration of cyclic guanosine 5′-monophosphate was elevated above control values in seizing rats by 57 to 170% in cerebellum, cortex, hippocampus, and striatum.

UR - http://www.scopus.com/inward/record.url?scp=0022921432&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022921432&partnerID=8YFLogxK

U2 - 10.1016/0014-4886(86)90200-1

DO - 10.1016/0014-4886(86)90200-1

M3 - Article

C2 - 3015661

AN - SCOPUS:0022921432

VL - 93

SP - 404

EP - 414

JO - Experimental Neurology

JF - Experimental Neurology

SN - 0014-4886

IS - 2

ER -