Nestin + progenitor cells isolated from adult human sweat gland stroma promote reepithelialisation and may stimulate angiogenesis in wounded human skin ex vivo

Tian Liao, Janin Lehmann, Sabine Sternstein, Arzu Yay, Guoyou Zhang, Anna Emilia Matthießen, Sandra Schumann, Frank Siemers, Charli Kruse, Jennifer E. Hundt, Ewan A. Langan, Stephan Tiede, Ralf Paus

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The combination of an aging population and an increasing prevalence of diseases associated with impaired-wound healing, including obesity, peripheral vascular disease and diabetes, is likely to result in a dramatic increase in the incidence and prevalence of chronic skin wounds. Indeed, systemic reviews are now not only trying to establish both the prevalence and the often under-estimated socio-economic costs of chronic skin wounds, but most importantly are addressing the impact that chronic wounds have on quality of life. Given the clear need for novel approaches to the management of chronic skin ulceration, ideally developed and tested in the human system in a manner that can be rapidly translated into clinical practice, we examined the effects of multipotent primary human nestin + progenitor cells on human wound healing in an ex vivo model. Human sweat gland-derived nestin + cells demonstrated the capacity to significantly promote two key wound healing parameters, i.e., both reepithelialisation and angiogenesis in experimentally wounded, organ-cultured human skin. The current data further support the use of full-thickness human skin wound-healing models ex vivo to pre-clinically test wound healing-promoting candidate agents. Whilst larger studies are required to substantiate a firm “proof-of-concept,” our preliminary studies encourage further efforts to systemically determine the potential of cell-based regenerative medicine strategies in general, and the use of skin appendage-associated human nestin + cells in particular, as novel treatment strategies for chronic skin ulceration.

Original languageEnglish (US)
JournalArchives of Dermatological Research
DOIs
StatePublished - Jan 1 2019

Fingerprint

Nestin
Sweat Glands
Stem Cells
Wound Healing
Skin
Wounds and Injuries
Regenerative Medicine
Peripheral Vascular Diseases
Obesity
Economics
Quality of Life
Costs and Cost Analysis
Incidence
Population

Keywords

  • Angiogenesis
  • CD31
  • Nestin
  • Organ culture
  • Reepithelialisation
  • Wound healing

ASJC Scopus subject areas

  • Dermatology

Cite this

Nestin + progenitor cells isolated from adult human sweat gland stroma promote reepithelialisation and may stimulate angiogenesis in wounded human skin ex vivo . / Liao, Tian; Lehmann, Janin; Sternstein, Sabine; Yay, Arzu; Zhang, Guoyou; Matthießen, Anna Emilia; Schumann, Sandra; Siemers, Frank; Kruse, Charli; Hundt, Jennifer E.; Langan, Ewan A.; Tiede, Stephan; Paus, Ralf.

In: Archives of Dermatological Research, 01.01.2019.

Research output: Contribution to journalArticle

Liao, Tian ; Lehmann, Janin ; Sternstein, Sabine ; Yay, Arzu ; Zhang, Guoyou ; Matthießen, Anna Emilia ; Schumann, Sandra ; Siemers, Frank ; Kruse, Charli ; Hundt, Jennifer E. ; Langan, Ewan A. ; Tiede, Stephan ; Paus, Ralf. / Nestin + progenitor cells isolated from adult human sweat gland stroma promote reepithelialisation and may stimulate angiogenesis in wounded human skin ex vivo In: Archives of Dermatological Research. 2019.
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abstract = "The combination of an aging population and an increasing prevalence of diseases associated with impaired-wound healing, including obesity, peripheral vascular disease and diabetes, is likely to result in a dramatic increase in the incidence and prevalence of chronic skin wounds. Indeed, systemic reviews are now not only trying to establish both the prevalence and the often under-estimated socio-economic costs of chronic skin wounds, but most importantly are addressing the impact that chronic wounds have on quality of life. Given the clear need for novel approaches to the management of chronic skin ulceration, ideally developed and tested in the human system in a manner that can be rapidly translated into clinical practice, we examined the effects of multipotent primary human nestin + progenitor cells on human wound healing in an ex vivo model. Human sweat gland-derived nestin + cells demonstrated the capacity to significantly promote two key wound healing parameters, i.e., both reepithelialisation and angiogenesis in experimentally wounded, organ-cultured human skin. The current data further support the use of full-thickness human skin wound-healing models ex vivo to pre-clinically test wound healing-promoting candidate agents. Whilst larger studies are required to substantiate a firm “proof-of-concept,” our preliminary studies encourage further efforts to systemically determine the potential of cell-based regenerative medicine strategies in general, and the use of skin appendage-associated human nestin + cells in particular, as novel treatment strategies for chronic skin ulceration.",
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AU - Liao, Tian

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AU - Yay, Arzu

AU - Zhang, Guoyou

AU - Matthießen, Anna Emilia

AU - Schumann, Sandra

AU - Siemers, Frank

AU - Kruse, Charli

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AU - Paus, Ralf

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