Nerve growth cone migration onto Schwann cells involves the calcium-dependent adhesion molecule, N-cadherin

Paul C. Letourneau, Terri A. Shattuck, Florence K. Roche, Masatoshi Takeichi, Vance Lemmon

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

The role of calcium-dependent adhesion molecules in the migration of nerve growth cones onto the top of Schwann cells was probed by examination of sensory growth cone-Schwann cell interactions in medium containing either 1.0 mM Ca2+ or 0.1 mM Ca2+. In the presence of 1.0 mM Ca2+ growth cones rapidly migrated onto Schwann cells, spread, and remained for extended periods. However, in 0.1 mM Ca2+ growth cones still made frequent contacts with Schwann cells, but migration onto the upper cell surface was much reduced. This contrast in growth cone-Schwann cell interactions could be switched rapidly by changing the Ca2+ concentration of the culture medium. Growth cones of retinal neurons showed similar calcium-dependence in their migration onto Schwann cells. Antibodies to the calcium-dependent adhesion molecule, N-cadherin, also blocked growth cone migration onto Schwann cells, but antibodies to another neuronal adhesion molecule, L1, had no effect on growth cone-Schwann cell interactions. Immunocytochemical staining for N-cadherin and L1 indicated that growth cones and Schwann cells have N-cadherin on their surfaces, while L1 is present only on axons and growth cones. These results provide two kinds of evidence that N-cadherin is important in the initial interactions of growth cones and Schwann cells.

Original languageEnglish
Pages (from-to)430-442
Number of pages13
JournalDevelopmental Biology
Volume138
Issue number2
StatePublished - Dec 1 1990
Externally publishedYes

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Growth Cones
Schwann Cells
Cadherins
Calcium
Cell Communication
Retinal Neurons
Antibodies
Cell Movement
Axons
Culture Media
Staining and Labeling

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Nerve growth cone migration onto Schwann cells involves the calcium-dependent adhesion molecule, N-cadherin. / Letourneau, Paul C.; Shattuck, Terri A.; Roche, Florence K.; Takeichi, Masatoshi; Lemmon, Vance.

In: Developmental Biology, Vol. 138, No. 2, 01.12.1990, p. 430-442.

Research output: Contribution to journalArticle

Letourneau, PC, Shattuck, TA, Roche, FK, Takeichi, M & Lemmon, V 1990, 'Nerve growth cone migration onto Schwann cells involves the calcium-dependent adhesion molecule, N-cadherin', Developmental Biology, vol. 138, no. 2, pp. 430-442.
Letourneau, Paul C. ; Shattuck, Terri A. ; Roche, Florence K. ; Takeichi, Masatoshi ; Lemmon, Vance. / Nerve growth cone migration onto Schwann cells involves the calcium-dependent adhesion molecule, N-cadherin. In: Developmental Biology. 1990 ; Vol. 138, No. 2. pp. 430-442.
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