Neonatal tolerance revisited again: Specific CTL priming in mouse neonates exposed to small numbers of semi- or fully allogeneic spleen cells

Rebecca D Adkins, Monica Jones, Yurong Bu, Robert B Levy

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Neonatal and adult mice mount distinct responses to allogeneic cells. Injection of neonates with fully allogeneic cells results in lethal graft-vs.-host disease (GVHD), whereas injection of semi-allogeneic (F1) cells leads to lifelong tolerance to the alloantigens, often marked by specific CTL non-responsiveness. In contrast, adults injected with the same number of either cell type become primed and develop vigorous anti-donor CTL activity. One possibility for this differential responsiveness may be developmental immaturity in the CTL arm of the immune system. Recent studies have shown that neonates are capable of mounting mature CTL responses, but only in the presence of strong Th1-promoting agents. Here, we demonstrate that neonates are competent to develop vigorous MHC class I-restricted CTL activity in vivo upon exposure to either fully or semi-allogeneic spleen cells. Specific CTL activity was generated using doses of cells approximately tenfold lower than levels used for the induction of GVHD or tolerance. Thus, the present studies demonstrate that mouse neonates are fully mature in their capacity to develop alloreactive CTL activity, as long as the dose of donor cells is low enough. These results have important implications for the known exposure of human fetuses and infants to small numbers of maternal cells.

Original languageEnglish
Pages (from-to)1901-1909
Number of pages9
JournalEuropean Journal of Immunology
Volume34
Issue number7
DOIs
StatePublished - Jul 1 2004

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Spleen
Newborn Infant
Cell Count
Tissue Donors
Injections
Isoantigens
Immune System
Fetus
Mothers
Transplants

Keywords

  • CTL
  • Graft-vs.-host disease
  • Ontogeny (comparative immunology)
  • Tolerance
  • Transplantation

ASJC Scopus subject areas

  • Immunology

Cite this

Neonatal tolerance revisited again : Specific CTL priming in mouse neonates exposed to small numbers of semi- or fully allogeneic spleen cells. / Adkins, Rebecca D; Jones, Monica; Bu, Yurong; Levy, Robert B.

In: European Journal of Immunology, Vol. 34, No. 7, 01.07.2004, p. 1901-1909.

Research output: Contribution to journalArticle

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