Neonatal porcine pancreatic cell clusters as a potential source for transplantation in humans: Characterization of proliferation, apoptosis, xenoantigen expression and gene delivery with recombinant AAV

Caterina Vizzardelli, Ruth Molano, Antonello Pileggi, Thierry Berney, Pierre Cattan, Elizabeth S. Fenjves, Alyson Peel, Christopher Fraker, Camillo Ricordi, Luca A Inverardi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Neonatal porcine islets are characterized by reproducible isolation success and high yields, sizable advantages over adult islets. In this work we have analyzed selected phenotypic and functional characteristics of porcine neonatal islets relevant to their possible use for transplant in humans. We show that porcine islet cells proliferate in culture, and synthesize and store islet-specific hormones. Proliferating beta cells can be easily identified. Implant of cultured neonatal islets in immunodeficient rodents results in the reversal of diabetes, albeit with delay. We also show that measurable apoptosis occurs in cultured neonatal porcine islets. Further, antigens recognized by human natural antibodies are expressed in a dynamic fashion over the culture period analyzed and are not limited to the alpha-Gal epitope. Lastly, we demonstrate that a recombinant Adeno-Associated virus can be used to efficiently deliver a reporter gene in porcine islets. This characterization might be helpful in the definition of the potential use of neonatal porcine islets for human transplantation.

Original languageEnglish
Pages (from-to)14-24
Number of pages11
JournalXenotransplantation
Volume9
Issue number1
DOIs
StatePublished - Jan 1 2002

Fingerprint

Heterophile Antigens
Swine
Transplantation
Apoptosis
Gene Expression
Islets of Langerhans Transplantation
Dependovirus
Islets of Langerhans
Reporter Genes
Epitopes
Rodentia
Hormones
Transplants
Antigens
Antibodies

Keywords

  • AAV
  • Gene delivery
  • Islets cell proliferation
  • Neonatal pig islets
  • Pig islets
  • Transduction
  • Xenoantigen expression

ASJC Scopus subject areas

  • Immunology

Cite this

Neonatal porcine pancreatic cell clusters as a potential source for transplantation in humans : Characterization of proliferation, apoptosis, xenoantigen expression and gene delivery with recombinant AAV. / Vizzardelli, Caterina; Molano, Ruth; Pileggi, Antonello; Berney, Thierry; Cattan, Pierre; Fenjves, Elizabeth S.; Peel, Alyson; Fraker, Christopher; Ricordi, Camillo; Inverardi, Luca A.

In: Xenotransplantation, Vol. 9, No. 1, 01.01.2002, p. 14-24.

Research output: Contribution to journalArticle

Vizzardelli, Caterina ; Molano, Ruth ; Pileggi, Antonello ; Berney, Thierry ; Cattan, Pierre ; Fenjves, Elizabeth S. ; Peel, Alyson ; Fraker, Christopher ; Ricordi, Camillo ; Inverardi, Luca A. / Neonatal porcine pancreatic cell clusters as a potential source for transplantation in humans : Characterization of proliferation, apoptosis, xenoantigen expression and gene delivery with recombinant AAV. In: Xenotransplantation. 2002 ; Vol. 9, No. 1. pp. 14-24.
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