Nef protein of HIV-1 has B-cell stimulatory activity

Narendra Chirmule, Naoki Oyaizu, Carl Saxinger, Savita G Pahwa

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Objective: To examine the B-cell stimulatory properties of the regulatory Nef protein of HIV-1. Methods: The effect of the HIV-1 regulatory proteins Nef, Tat and Vif, were analyzed for their ability to induce differentiation of normal B lymphocytes into immunoglobulin secreting cells (ISC). Results: A recombinant Nef protein, but neither Tat or Vif, was able to induce ISC in peripheral blood lymphocyte (PBL) cultures of HIV-1-seronegative donors. Another recombinant Nef protein, d-Nef, with a truncated amino terminal (deletion of 34 amino acids) failed to induce B-cell differentiation. Pretreatment of the Nef protein with a polyclonal anti-Nef-antibody abrogated its B-cell stimulatory activity. The Nef-induced B-cell differentiation was dependent on cell-to-cell contact. Cell surface molecules leukocyte function-associated molecule (LFA)-1, intracellular adhesion molecule (ICAM)-1, human lymphocyte antigen-DR and B7 were involved in the T-B-cell interaction because monoclonal antibodies to these molecules abrogated the Nef-induced B-cell differentiation response. The Nef protein was able to induce interleukin (IL)-6 messenger (m)RNA and IL-6 protein secretion in PBL, with monocytes as the primary source. Conclusions: These findings indicate that regulatory (Nef) proteins of HIV-1 contribute to the intense B-cell activation that occurs in association with HIV-1 infection. T-B-cell contact-dependent interaction and induction of IL-6 by these proteins appear to play major roles in this process.

Original languageEnglish
Pages (from-to)733-739
Number of pages7
JournalAIDS
Volume8
Issue number6
StatePublished - Jun 1 1994
Externally publishedYes

Fingerprint

B-Lymphocytes
nef Gene Products
Cell Differentiation
Interleukin-6
Antibody-Producing Cells
Lymphocytes
Recombinant Proteins
HIV-1
B7 Antigens
Human immunodeficiency virus 1 nef protein
Cell Communication
HIV Infections
Monocytes
Anti-Idiotypic Antibodies
Proteins
Leukocytes
Monoclonal Antibodies
Amino Acids
Messenger RNA

Keywords

  • B-cell differentiation
  • Interleukin-6
  • Nef
  • T-B-cell contact

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Chirmule, N., Oyaizu, N., Saxinger, C., & Pahwa, S. G. (1994). Nef protein of HIV-1 has B-cell stimulatory activity. AIDS, 8(6), 733-739.

Nef protein of HIV-1 has B-cell stimulatory activity. / Chirmule, Narendra; Oyaizu, Naoki; Saxinger, Carl; Pahwa, Savita G.

In: AIDS, Vol. 8, No. 6, 01.06.1994, p. 733-739.

Research output: Contribution to journalArticle

Chirmule, N, Oyaizu, N, Saxinger, C & Pahwa, SG 1994, 'Nef protein of HIV-1 has B-cell stimulatory activity', AIDS, vol. 8, no. 6, pp. 733-739.
Chirmule N, Oyaizu N, Saxinger C, Pahwa SG. Nef protein of HIV-1 has B-cell stimulatory activity. AIDS. 1994 Jun 1;8(6):733-739.
Chirmule, Narendra ; Oyaizu, Naoki ; Saxinger, Carl ; Pahwa, Savita G. / Nef protein of HIV-1 has B-cell stimulatory activity. In: AIDS. 1994 ; Vol. 8, No. 6. pp. 733-739.
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