Nature of the antigenic complex recognized by T lymphocytes. IX. Direct immunochemical demonstration of nominal antigen on the macrophage cell surface

Thomas Malek, E. M. Shevach

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7 Citations (Scopus)

Abstract

As an initial approach to macrophage (MΦ) antigen handling, guinea pig MΦ pulsed with the radiolabeled terpolymer L-glutamic acid, L-lysine, L-tyrosine (GLT) were modified with the cell surface-specific probe 2,4,6-trinitrobenzenesulfonic acid either immediately after pulsing (fresh) or 24 h after pulsing (aged); nonidet P-40 extracts were prepared from these cells and analysis of these extracts with anti-trinitrophenyl antisera was performed in a quantitative radioimmunoassay. Such experiments indicated that fresh GLT-pulsed MΦ contained a pool of intracellular GLT as well as a distinct pool of cell surface GLT. In contrast, aged GLT-pulsed MΦ lacked intracellular GLT but expressed GLT on the cell surface. In addition, a pool of cell surface GLT was also detected on Ia-MΦ and on MΦ from guinea pigs which were nonresponders to GLT. Thus, this study provides a direct demonstration of the presence of exogenous antigen on the MΦ cell surface. However, the cell surface expression of GLT does not appear to be an exclusive property expressed by functional Ia+ antigen-presenting MΦ.

Original languageEnglish
Pages (from-to)825-831
Number of pages7
JournalEuropean Journal of Immunology
Volume12
Issue number10
StatePublished - Dec 1 1982
Externally publishedYes

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Lysine
Tyrosine
Glutamic Acid
Macrophages
T-Lymphocytes
Antigens
Guinea Pigs
Trinitrobenzenesulfonic Acid
Histocompatibility Antigens Class II
Cell Extracts
Radioimmunoassay
Immune Sera

ASJC Scopus subject areas

  • Immunology

Cite this

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abstract = "As an initial approach to macrophage (MΦ) antigen handling, guinea pig MΦ pulsed with the radiolabeled terpolymer L-glutamic acid, L-lysine, L-tyrosine (GLT) were modified with the cell surface-specific probe 2,4,6-trinitrobenzenesulfonic acid either immediately after pulsing (fresh) or 24 h after pulsing (aged); nonidet P-40 extracts were prepared from these cells and analysis of these extracts with anti-trinitrophenyl antisera was performed in a quantitative radioimmunoassay. Such experiments indicated that fresh GLT-pulsed MΦ contained a pool of intracellular GLT as well as a distinct pool of cell surface GLT. In contrast, aged GLT-pulsed MΦ lacked intracellular GLT but expressed GLT on the cell surface. In addition, a pool of cell surface GLT was also detected on Ia-MΦ and on MΦ from guinea pigs which were nonresponders to GLT. Thus, this study provides a direct demonstration of the presence of exogenous antigen on the MΦ cell surface. However, the cell surface expression of GLT does not appear to be an exclusive property expressed by functional Ia+ antigen-presenting MΦ.",
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