Natural anti-IgG antibodies to autologous and heterologous species IgG

L. Fuller, M. Carreno, V. Esquenazi, David Roth, J. Miller

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In the present report we have isolated by affinity chromatography a putative human Fab2 that binds to human Fab2. We have examined the binding specificity of the Fab2 anti-Fab2 to IgG, Fab2, Fab, and Fc fragments derived from various species IgG and have attempted to isolate species-specific antibody by affinity chromatography. The results obtained in this study using 125I-Fab2 anti-Fab2 demonstrate that the natural antihuman antibodies cannot be separated into IgG species-specific population. Moreover, subjecting affinity-isolated Fab2 anti-Fab2 to a Sepharose 4B-mouse IgG column failed to remove mouse IgG-specific binding activity. These data suggest that the human natural antibodies, ie, Fab2 anti-Fab2 recognize homology epitopes (domain) and not species-specific IgG epitopes. The observation that Fab2 anti-Fab2 reacts with the Fc fragment as well as Fab supports this hypothesis. Additionally, the binding specificity of the Fab2 that was bound (fractions 30-35) by the Sepharose 4B-mouse IgG column exhibited the same reactivity as the nonbound fractions 5,6. The observation that Fab2 that were bound by the Sepharose 4B-mouse IgG suggest that this population contained molecules with higher binding affinities than the Fab2 in fractions 5,6.

Original languageEnglish
Pages (from-to)45-46
Number of pages2
JournalTransplantation Proceedings
Volume20
Issue number1 SUPPL. 1
StatePublished - Jan 1 1988

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Anti-Idiotypic Antibodies
Immunoglobulin G
Sepharose
Immunoglobulin Fc Fragments
Affinity Chromatography
Epitopes
Immunoglobulin Fab Fragments
Antibody Affinity
anti-IgG
Antibodies
Population

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

Fuller, L., Carreno, M., Esquenazi, V., Roth, D., & Miller, J. (1988). Natural anti-IgG antibodies to autologous and heterologous species IgG. Transplantation Proceedings, 20(1 SUPPL. 1), 45-46.

Natural anti-IgG antibodies to autologous and heterologous species IgG. / Fuller, L.; Carreno, M.; Esquenazi, V.; Roth, David; Miller, J.

In: Transplantation Proceedings, Vol. 20, No. 1 SUPPL. 1, 01.01.1988, p. 45-46.

Research output: Contribution to journalArticle

Fuller, L, Carreno, M, Esquenazi, V, Roth, D & Miller, J 1988, 'Natural anti-IgG antibodies to autologous and heterologous species IgG', Transplantation Proceedings, vol. 20, no. 1 SUPPL. 1, pp. 45-46.
Fuller L, Carreno M, Esquenazi V, Roth D, Miller J. Natural anti-IgG antibodies to autologous and heterologous species IgG. Transplantation Proceedings. 1988 Jan 1;20(1 SUPPL. 1):45-46.
Fuller, L. ; Carreno, M. ; Esquenazi, V. ; Roth, David ; Miller, J. / Natural anti-IgG antibodies to autologous and heterologous species IgG. In: Transplantation Proceedings. 1988 ; Vol. 20, No. 1 SUPPL. 1. pp. 45-46.
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