Na+/H+ antiport is essential for yersinia pestis virulence

Yusuke Minato, Amit Ghosh, Wyatt J. Faulkner, Erin J. Lind, Sara Schesser Bartra, Gregory V Plano, Clayton O. Jarrett, B. Joseph Hinnebusch, Judith Winogrodzki, Pavel Dibrov, Claudia C. Häse

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Na+/H+ antiporters are ubiquitous membrane proteins that play a central role in the ion homeostasis of cells. In this study, we examined the possible role of Na+/H+ antiport in Yersinia pestis virulence and found that Y. pestis strains lacking the major Na+/H+ antiporters, NhaA and NhaB, are completely attenuated in an in vivo model of plague. The Y. pestis derivative strain lacking the nhaA and nhaB genes showed markedly decreased survival in blood and blood serum ex vivo. Complementation of either nhaA or nhaB in trans restored the survival of the Y. pestis nhaA nhaB double deletion mutant in blood. The nhaA nhaB double deletion mutant also showed inhibited growth in an artificial serum medium, Opti-MEM, and a rich LB-based medium with Na+ levels and pH values similar to those for blood. Taken together, these data strongly suggest that intact Na+/H+ antiport is indispensable for the survival of Y. pestis in the bloodstreams of infected animals and thus might be regarded as a promising noncanonical drug target for infections caused by Y. pestis and possibly for those caused by other blood-borne bacterial pathogens.

Original languageEnglish
Pages (from-to)3163-3172
Number of pages10
JournalInfection and Immunity
Volume81
Issue number9
DOIs
StatePublished - Sep 23 2013

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Yersinia pestis
Ion Transport
Virulence
Sodium-Hydrogen Antiporter
Blood-Borne Pathogens
Plague
Serum
Membrane Proteins
Homeostasis
Ions
Growth
Infection
Pharmaceutical Preparations
Genes

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Minato, Y., Ghosh, A., Faulkner, W. J., Lind, E. J., Bartra, S. S., Plano, G. V., ... Häse, C. C. (2013). Na+/H+ antiport is essential for yersinia pestis virulence. Infection and Immunity, 81(9), 3163-3172. https://doi.org/10.1128/IAI.00071-13

Na+/H+ antiport is essential for yersinia pestis virulence. / Minato, Yusuke; Ghosh, Amit; Faulkner, Wyatt J.; Lind, Erin J.; Bartra, Sara Schesser; Plano, Gregory V; Jarrett, Clayton O.; Hinnebusch, B. Joseph; Winogrodzki, Judith; Dibrov, Pavel; Häse, Claudia C.

In: Infection and Immunity, Vol. 81, No. 9, 23.09.2013, p. 3163-3172.

Research output: Contribution to journalArticle

Minato, Y, Ghosh, A, Faulkner, WJ, Lind, EJ, Bartra, SS, Plano, GV, Jarrett, CO, Hinnebusch, BJ, Winogrodzki, J, Dibrov, P & Häse, CC 2013, 'Na+/H+ antiport is essential for yersinia pestis virulence', Infection and Immunity, vol. 81, no. 9, pp. 3163-3172. https://doi.org/10.1128/IAI.00071-13
Minato Y, Ghosh A, Faulkner WJ, Lind EJ, Bartra SS, Plano GV et al. Na+/H+ antiport is essential for yersinia pestis virulence. Infection and Immunity. 2013 Sep 23;81(9):3163-3172. https://doi.org/10.1128/IAI.00071-13
Minato, Yusuke ; Ghosh, Amit ; Faulkner, Wyatt J. ; Lind, Erin J. ; Bartra, Sara Schesser ; Plano, Gregory V ; Jarrett, Clayton O. ; Hinnebusch, B. Joseph ; Winogrodzki, Judith ; Dibrov, Pavel ; Häse, Claudia C. / Na+/H+ antiport is essential for yersinia pestis virulence. In: Infection and Immunity. 2013 ; Vol. 81, No. 9. pp. 3163-3172.
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