Nanos1 functions as a translational repressor in the Xenopus germline

Fangfang Lai, Yi Zhou, Xueting Luo, Josh Fox, Mary Lou L King

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Nanos family members have been shown to act as translational repressors in the Drosophila and Caenorhabditis elegans germline, but direct evidence is missing for a similar function in vertebrates. Using a tethered function assay, we show that Xenopus Nanos1 is a translational repressor and that association with the RNA is required for this repression. We identified a 14 amino acid region within the N-terminal domain of Nanos1 that is conserved in organisms as diverse as sponge and Human. The region is found in all vertebrates but notably lacking in Drosophila and C. elegans. Deletion and substitution analysis revealed that this conserved region was required for Nanos1 repressive activity. Consistent with this observation, deletion of this region was sufficient to prevent abnormal development that results from ectopic expression of Nanos1 in oocytes. Although Nanos1 can repress capped and polyadenylated RNAs, Nanos1 mediated repression did not require the targeted RNA to have a cap or to be polyadenylated. These results suggest that Nanos1 is capable of repressing translation by several different mechanisms. We found that Nanos1, like Drosophila Nanos, associates with cyclin B1 RNA in vivo indicating that some Nanos targets may be evolutionarily conserved. Nanos1 protein was detected and thus available to repress mRNAs while PGCs were in the endoderm, but was not observed in PGCs after this stage.

Original languageEnglish
Pages (from-to)153-163
Number of pages11
JournalMechanisms of Development
Volume128
Issue number1-2
DOIs
StatePublished - Jan 1 2011

Fingerprint

Xenopus
Drosophila
Caenorhabditis elegans
RNA
Vertebrates
Cyclin B1
Messenger RNA
Endoderm
Porifera
Oocytes
Amino Acids
Proteins

Keywords

  • Germline determination
  • Nanos1/Xcat2
  • Translational repression
  • Xenopus

ASJC Scopus subject areas

  • Developmental Biology
  • Embryology

Cite this

Nanos1 functions as a translational repressor in the Xenopus germline. / Lai, Fangfang; Zhou, Yi; Luo, Xueting; Fox, Josh; King, Mary Lou L.

In: Mechanisms of Development, Vol. 128, No. 1-2, 01.01.2011, p. 153-163.

Research output: Contribution to journalArticle

Lai, Fangfang ; Zhou, Yi ; Luo, Xueting ; Fox, Josh ; King, Mary Lou L. / Nanos1 functions as a translational repressor in the Xenopus germline. In: Mechanisms of Development. 2011 ; Vol. 128, No. 1-2. pp. 153-163.
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