Abstract
This work reports a biosensor for monitoring xanthine for potential wound healing assessment. Active substrate of the biosensor has xanthine oxidase (XO) and horseradish peroxidase (HRP) physisorbed on a nanocomposite of multiwalled carbon nanotubes (MWCNT) decorated with gold nanoparticles (AuNP). The presence of HRP provided a two-fold increase in response to xanthine, and a three-fold increase in response to the nanocomposite. With a sensitivity of 155.71 nA μM−1 cm−2 the biosensor offers a detection limit of 1.3 μM, with linear response between 22 μM and 0.4 mM. Clinical sample analyses showed the feasibility of xanthine detection from biofluids in a lesion site due to diffusion of the analyte into surrounding biofluids. Higher concentrations by three-fold were observed from wound proximity, than away from injury, with an average recovery of 110%. Results show the feasibility of monitoring wound severity through longitudinal measurements of xanthine from injured vicinity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | B3295-B3301 |
| Journal | Journal of the Electrochemical Society |
| Volume | 166 |
| Issue number | 9 |
| DOIs | |
| State | Published - Jan 1 2019 |
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ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Renewable Energy, Sustainability and the Environment
- Surfaces, Coatings and Films
- Electrochemistry
- Materials Chemistry
Cite this
Nanocomposite bienzymatic sensor for monitoring xanthine in wound diagnostics. / RoyChoudhury, Sohini; Umasankar, Yogeswaran; Bhushan, Pulak; Hirt, Penelope A.; MacQuhae, Flor E.; Borda, Luis J.; Lev-Tov, Hadar A.; Kirsner, Robert; Bhansali, Shekhar.
In: Journal of the Electrochemical Society, Vol. 166, No. 9, 01.01.2019, p. B3295-B3301.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nanocomposite bienzymatic sensor for monitoring xanthine in wound diagnostics
AU - RoyChoudhury, Sohini
AU - Umasankar, Yogeswaran
AU - Bhushan, Pulak
AU - Hirt, Penelope A.
AU - MacQuhae, Flor E.
AU - Borda, Luis J.
AU - Lev-Tov, Hadar A.
AU - Kirsner, Robert
AU - Bhansali, Shekhar
PY - 2019/1/1
Y1 - 2019/1/1
N2 - This work reports a biosensor for monitoring xanthine for potential wound healing assessment. Active substrate of the biosensor has xanthine oxidase (XO) and horseradish peroxidase (HRP) physisorbed on a nanocomposite of multiwalled carbon nanotubes (MWCNT) decorated with gold nanoparticles (AuNP). The presence of HRP provided a two-fold increase in response to xanthine, and a three-fold increase in response to the nanocomposite. With a sensitivity of 155.71 nA μM−1 cm−2 the biosensor offers a detection limit of 1.3 μM, with linear response between 22 μM and 0.4 mM. Clinical sample analyses showed the feasibility of xanthine detection from biofluids in a lesion site due to diffusion of the analyte into surrounding biofluids. Higher concentrations by three-fold were observed from wound proximity, than away from injury, with an average recovery of 110%. Results show the feasibility of monitoring wound severity through longitudinal measurements of xanthine from injured vicinity.
AB - This work reports a biosensor for monitoring xanthine for potential wound healing assessment. Active substrate of the biosensor has xanthine oxidase (XO) and horseradish peroxidase (HRP) physisorbed on a nanocomposite of multiwalled carbon nanotubes (MWCNT) decorated with gold nanoparticles (AuNP). The presence of HRP provided a two-fold increase in response to xanthine, and a three-fold increase in response to the nanocomposite. With a sensitivity of 155.71 nA μM−1 cm−2 the biosensor offers a detection limit of 1.3 μM, with linear response between 22 μM and 0.4 mM. Clinical sample analyses showed the feasibility of xanthine detection from biofluids in a lesion site due to diffusion of the analyte into surrounding biofluids. Higher concentrations by three-fold were observed from wound proximity, than away from injury, with an average recovery of 110%. Results show the feasibility of monitoring wound severity through longitudinal measurements of xanthine from injured vicinity.
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UR - http://www.scopus.com/inward/citedby.url?scp=85071527180&partnerID=8YFLogxK
U2 - 10.1149/2.0401909jes
DO - 10.1149/2.0401909jes
M3 - Article
AN - SCOPUS:85071527180
VL - 166
SP - B3295-B3301
JO - Journal of the Electrochemical Society
JF - Journal of the Electrochemical Society
SN - 0013-4651
IS - 9
ER -