Nanocomposite bienzymatic sensor for monitoring xanthine in wound diagnostics

Sohini RoyChoudhury, Yogeswaran Umasankar, Pulak Bhushan, Penelope A. Hirt, Flor E. MacQuhae, Luis J. Borda, Hadar A. Lev-Tov, Robert Kirsner, Shekhar Bhansali

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

This work reports a biosensor for monitoring xanthine for potential wound healing assessment. Active substrate of the biosensor has xanthine oxidase (XO) and horseradish peroxidase (HRP) physisorbed on a nanocomposite of multiwalled carbon nanotubes (MWCNT) decorated with gold nanoparticles (AuNP). The presence of HRP provided a two-fold increase in response to xanthine, and a three-fold increase in response to the nanocomposite. With a sensitivity of 155.71 nA μM−1 cm−2 the biosensor offers a detection limit of 1.3 μM, with linear response between 22 μM and 0.4 mM. Clinical sample analyses showed the feasibility of xanthine detection from biofluids in a lesion site due to diffusion of the analyte into surrounding biofluids. Higher concentrations by three-fold were observed from wound proximity, than away from injury, with an average recovery of 110%. Results show the feasibility of monitoring wound severity through longitudinal measurements of xanthine from injured vicinity.

Original languageEnglish (US)
Pages (from-to)B3295-B3301
JournalJournal of the Electrochemical Society
Volume166
Issue number9
DOIs
StatePublished - Jan 1 2019

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Renewable Energy, Sustainability and the Environment
  • Surfaces, Coatings and Films
  • Electrochemistry
  • Materials Chemistry

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    RoyChoudhury, S., Umasankar, Y., Bhushan, P., Hirt, P. A., MacQuhae, F. E., Borda, L. J., Lev-Tov, H. A., Kirsner, R., & Bhansali, S. (2019). Nanocomposite bienzymatic sensor for monitoring xanthine in wound diagnostics. Journal of the Electrochemical Society, 166(9), B3295-B3301. https://doi.org/10.1149/2.0401909jes