Nanocomposite bienzymatic sensor for monitoring xanthine in wound diagnostics

Sohini RoyChoudhury, Yogeswaran Umasankar, Pulak Bhushan, Penelope A. Hirt, Flor E. MacQuhae, Luis J. Borda, Hadar A. Lev-Tov, Robert Kirsner, Shekhar Bhansali

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This work reports a biosensor for monitoring xanthine for potential wound healing assessment. Active substrate of the biosensor has xanthine oxidase (XO) and horseradish peroxidase (HRP) physisorbed on a nanocomposite of multiwalled carbon nanotubes (MWCNT) decorated with gold nanoparticles (AuNP). The presence of HRP provided a two-fold increase in response to xanthine, and a three-fold increase in response to the nanocomposite. With a sensitivity of 155.71 nA μM−1 cm−2 the biosensor offers a detection limit of 1.3 μM, with linear response between 22 μM and 0.4 mM. Clinical sample analyses showed the feasibility of xanthine detection from biofluids in a lesion site due to diffusion of the analyte into surrounding biofluids. Higher concentrations by three-fold were observed from wound proximity, than away from injury, with an average recovery of 110%. Results show the feasibility of monitoring wound severity through longitudinal measurements of xanthine from injured vicinity.

Original languageEnglish (US)
Pages (from-to)B3295-B3301
JournalJournal of the Electrochemical Society
Volume166
Issue number9
DOIs
StatePublished - Jan 1 2019

Fingerprint

Xanthine
Biosensors
Nanocomposites
Monitoring
Sensors
Horseradish Peroxidase
Multiwalled carbon nanotubes (MWCN)
Xanthine Oxidase
Gold
Nanoparticles
Recovery
Substrates

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Renewable Energy, Sustainability and the Environment
  • Surfaces, Coatings and Films
  • Electrochemistry
  • Materials Chemistry

Cite this

RoyChoudhury, S., Umasankar, Y., Bhushan, P., Hirt, P. A., MacQuhae, F. E., Borda, L. J., ... Bhansali, S. (2019). Nanocomposite bienzymatic sensor for monitoring xanthine in wound diagnostics. Journal of the Electrochemical Society, 166(9), B3295-B3301. https://doi.org/10.1149/2.0401909jes

Nanocomposite bienzymatic sensor for monitoring xanthine in wound diagnostics. / RoyChoudhury, Sohini; Umasankar, Yogeswaran; Bhushan, Pulak; Hirt, Penelope A.; MacQuhae, Flor E.; Borda, Luis J.; Lev-Tov, Hadar A.; Kirsner, Robert; Bhansali, Shekhar.

In: Journal of the Electrochemical Society, Vol. 166, No. 9, 01.01.2019, p. B3295-B3301.

Research output: Contribution to journalArticle

RoyChoudhury, S, Umasankar, Y, Bhushan, P, Hirt, PA, MacQuhae, FE, Borda, LJ, Lev-Tov, HA, Kirsner, R & Bhansali, S 2019, 'Nanocomposite bienzymatic sensor for monitoring xanthine in wound diagnostics', Journal of the Electrochemical Society, vol. 166, no. 9, pp. B3295-B3301. https://doi.org/10.1149/2.0401909jes
RoyChoudhury S, Umasankar Y, Bhushan P, Hirt PA, MacQuhae FE, Borda LJ et al. Nanocomposite bienzymatic sensor for monitoring xanthine in wound diagnostics. Journal of the Electrochemical Society. 2019 Jan 1;166(9):B3295-B3301. https://doi.org/10.1149/2.0401909jes
RoyChoudhury, Sohini ; Umasankar, Yogeswaran ; Bhushan, Pulak ; Hirt, Penelope A. ; MacQuhae, Flor E. ; Borda, Luis J. ; Lev-Tov, Hadar A. ; Kirsner, Robert ; Bhansali, Shekhar. / Nanocomposite bienzymatic sensor for monitoring xanthine in wound diagnostics. In: Journal of the Electrochemical Society. 2019 ; Vol. 166, No. 9. pp. B3295-B3301.
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