Nanocomposite bienzymatic sensor for monitoring xanthine in wound diagnostics

Sohini RoyChoudhury; Yogeswaran Umasankar; Pulak Bhushan; Penelope A. Hirt; Flor E. MacQuhae; Luis J. Borda; Hadar A. Lev-Tov; Robert Kirsner; Shekhar Bhansali

doi:10.1149/2.0401909jes

Nanocomposite bienzymatic sensor for monitoring xanthine in wound diagnostics

Sohini RoyChoudhury, Yogeswaran Umasankar, Pulak Bhushan, Penelope A. Hirt, Flor E. MacQuhae, Luis J. Borda, Hadar A. Lev-Tov, Robert Kirsner, Shekhar Bhansali

Dermatology & Cutaneous Surgery

Research output: Contribution to journal › Article

1 Scopus citations

Abstract

This work reports a biosensor for monitoring xanthine for potential wound healing assessment. Active substrate of the biosensor has xanthine oxidase (XO) and horseradish peroxidase (HRP) physisorbed on a nanocomposite of multiwalled carbon nanotubes (MWCNT) decorated with gold nanoparticles (AuNP). The presence of HRP provided a two-fold increase in response to xanthine, and a three-fold increase in response to the nanocomposite. With a sensitivity of 155.71 nA μM−¹ cm⁻² the biosensor offers a detection limit of 1.3 μM, with linear response between 22 μM and 0.4 mM. Clinical sample analyses showed the feasibility of xanthine detection from biofluids in a lesion site due to diffusion of the analyte into surrounding biofluids. Higher concentrations by three-fold were observed from wound proximity, than away from injury, with an average recovery of 110%. Results show the feasibility of monitoring wound severity through longitudinal measurements of xanthine from injured vicinity.

Original language	English (US)
Pages (from-to)	B3295-B3301
Journal	Journal of the Electrochemical Society
Volume	166
Issue number	9
DOIs	https://doi.org/10.1149/2.0401909jes
State	Published - Jan 1 2019

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Renewable Energy, Sustainability and the Environment
Surfaces, Coatings and Films
Electrochemistry
Materials Chemistry

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RoyChoudhury, S., Umasankar, Y., Bhushan, P., Hirt, P. A., MacQuhae, F. E., Borda, L. J., Lev-Tov, H. A., Kirsner, R., & Bhansali, S. (2019). Nanocomposite bienzymatic sensor for monitoring xanthine in wound diagnostics. Journal of the Electrochemical Society, 166(9), B3295-B3301. https://doi.org/10.1149/2.0401909jes

Nanocomposite bienzymatic sensor for monitoring xanthine in wound diagnostics. / RoyChoudhury, Sohini; Umasankar, Yogeswaran; Bhushan, Pulak; Hirt, Penelope A.; MacQuhae, Flor E.; Borda, Luis J.; Lev-Tov, Hadar A.; Kirsner, Robert; Bhansali, Shekhar.

In: Journal of the Electrochemical Society, Vol. 166, No. 9, 01.01.2019, p. B3295-B3301.

Research output: Contribution to journal › Article

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abstract = "This work reports a biosensor for monitoring xanthine for potential wound healing assessment. Active substrate of the biosensor has xanthine oxidase (XO) and horseradish peroxidase (HRP) physisorbed on a nanocomposite of multiwalled carbon nanotubes (MWCNT) decorated with gold nanoparticles (AuNP). The presence of HRP provided a two-fold increase in response to xanthine, and a three-fold increase in response to the nanocomposite. With a sensitivity of 155.71 nA μM−1 cm−2 the biosensor offers a detection limit of 1.3 μM, with linear response between 22 μM and 0.4 mM. Clinical sample analyses showed the feasibility of xanthine detection from biofluids in a lesion site due to diffusion of the analyte into surrounding biofluids. Higher concentrations by three-fold were observed from wound proximity, than away from injury, with an average recovery of 110%. Results show the feasibility of monitoring wound severity through longitudinal measurements of xanthine from injured vicinity.",

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