TY - JOUR
T1 - Nanocomposite bienzymatic sensor for monitoring xanthine in wound diagnostics
AU - RoyChoudhury, Sohini
AU - Umasankar, Yogeswaran
AU - Bhushan, Pulak
AU - Hirt, Penelope A.
AU - MacQuhae, Flor E.
AU - Borda, Luis J.
AU - Lev-Tov, Hadar A.
AU - Kirsner, Robert
AU - Bhansali, Shekhar
N1 - Funding Information:
This work is being supported by the NSF Nanosystems Engineering Research Centre for Advanced Self-Powered Systems of Integrated Biosensors and Technologies (ASSIST) under Award Number EEC-1160483.
Publisher Copyright:
© The Author(s) 2019.
PY - 2019
Y1 - 2019
N2 - This work reports a biosensor for monitoring xanthine for potential wound healing assessment. Active substrate of the biosensor has xanthine oxidase (XO) and horseradish peroxidase (HRP) physisorbed on a nanocomposite of multiwalled carbon nanotubes (MWCNT) decorated with gold nanoparticles (AuNP). The presence of HRP provided a two-fold increase in response to xanthine, and a three-fold increase in response to the nanocomposite. With a sensitivity of 155.71 nA μM−1 cm−2 the biosensor offers a detection limit of 1.3 μM, with linear response between 22 μM and 0.4 mM. Clinical sample analyses showed the feasibility of xanthine detection from biofluids in a lesion site due to diffusion of the analyte into surrounding biofluids. Higher concentrations by three-fold were observed from wound proximity, than away from injury, with an average recovery of 110%. Results show the feasibility of monitoring wound severity through longitudinal measurements of xanthine from injured vicinity.
AB - This work reports a biosensor for monitoring xanthine for potential wound healing assessment. Active substrate of the biosensor has xanthine oxidase (XO) and horseradish peroxidase (HRP) physisorbed on a nanocomposite of multiwalled carbon nanotubes (MWCNT) decorated with gold nanoparticles (AuNP). The presence of HRP provided a two-fold increase in response to xanthine, and a three-fold increase in response to the nanocomposite. With a sensitivity of 155.71 nA μM−1 cm−2 the biosensor offers a detection limit of 1.3 μM, with linear response between 22 μM and 0.4 mM. Clinical sample analyses showed the feasibility of xanthine detection from biofluids in a lesion site due to diffusion of the analyte into surrounding biofluids. Higher concentrations by three-fold were observed from wound proximity, than away from injury, with an average recovery of 110%. Results show the feasibility of monitoring wound severity through longitudinal measurements of xanthine from injured vicinity.
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U2 - 10.1149/2.0401909jes
DO - 10.1149/2.0401909jes
M3 - Article
AN - SCOPUS:85071527180
VL - 166
SP - B3295-B3301
JO - Journal of the Electrochemical Society
JF - Journal of the Electrochemical Society
SN - 0013-4651
IS - 9
ER -