Abstract
N-Cadherin is a major cell adhesion molecule that is expressed in the developing nervous system where it has been implicated in neural migration and axon growth. Recently, a role for N-cadherin in oligodendrocyte differentiation has been identified. Oligodendrocyte precursors adhere to N-cadherin and mature rapidly to produce myelin sheets. Since this implies that oligodendrocytes express N-cadherin, we examined the expression of N-cadherin by oligodendrocytes in culture. N-Cadherin was expressed by O-2A progenitors, immature oligodendrocytes and mature oligodendrocytes, but at a lower level than in type 1 astrocytes in the same cultures. On mature oligodendrocytes, the N-cadherin was concentrated on the major processes emerging from the soma. The ability of N-cadherin and merosin to promote oligodendrocyte precursor migration was also studied. Average migration rates were significantly higher on merosin (11.2 μm/h) than on N-cadherin (5.6 μm/h). These results suggest that N-cadherin is not likely to function predominantly as a substrate that stimulates migration of O-2A progenitors, but may be more important in initiating early oligodendrocyte-axon interactions that promote the process of myelination.
| Original language | English |
|---|---|
| Pages (from-to) | 9-15 |
| Number of pages | 7 |
| Journal | Developmental Brain Research |
| Volume | 97 |
| Issue number | 1 |
| DOIs | |
| State | Published - Nov 22 1996 |
| Externally published | Yes |
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Keywords
- Cell migration
- Immunofluorescence
- Merosin
- Myelination
- O-2A progenitor
ASJC Scopus subject areas
- Developmental Biology
- Developmental Neuroscience
Cite this
N-Cadherin expression and function in cultured oligodendrocytes. / Payne, Harold Ross; Hemperly, John J.; Lemmon, Vance.
In: Developmental Brain Research, Vol. 97, No. 1, 22.11.1996, p. 9-15.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - N-Cadherin expression and function in cultured oligodendrocytes
AU - Payne, Harold Ross
AU - Hemperly, John J.
AU - Lemmon, Vance
PY - 1996/11/22
Y1 - 1996/11/22
N2 - N-Cadherin is a major cell adhesion molecule that is expressed in the developing nervous system where it has been implicated in neural migration and axon growth. Recently, a role for N-cadherin in oligodendrocyte differentiation has been identified. Oligodendrocyte precursors adhere to N-cadherin and mature rapidly to produce myelin sheets. Since this implies that oligodendrocytes express N-cadherin, we examined the expression of N-cadherin by oligodendrocytes in culture. N-Cadherin was expressed by O-2A progenitors, immature oligodendrocytes and mature oligodendrocytes, but at a lower level than in type 1 astrocytes in the same cultures. On mature oligodendrocytes, the N-cadherin was concentrated on the major processes emerging from the soma. The ability of N-cadherin and merosin to promote oligodendrocyte precursor migration was also studied. Average migration rates were significantly higher on merosin (11.2 μm/h) than on N-cadherin (5.6 μm/h). These results suggest that N-cadherin is not likely to function predominantly as a substrate that stimulates migration of O-2A progenitors, but may be more important in initiating early oligodendrocyte-axon interactions that promote the process of myelination.
AB - N-Cadherin is a major cell adhesion molecule that is expressed in the developing nervous system where it has been implicated in neural migration and axon growth. Recently, a role for N-cadherin in oligodendrocyte differentiation has been identified. Oligodendrocyte precursors adhere to N-cadherin and mature rapidly to produce myelin sheets. Since this implies that oligodendrocytes express N-cadherin, we examined the expression of N-cadherin by oligodendrocytes in culture. N-Cadherin was expressed by O-2A progenitors, immature oligodendrocytes and mature oligodendrocytes, but at a lower level than in type 1 astrocytes in the same cultures. On mature oligodendrocytes, the N-cadherin was concentrated on the major processes emerging from the soma. The ability of N-cadherin and merosin to promote oligodendrocyte precursor migration was also studied. Average migration rates were significantly higher on merosin (11.2 μm/h) than on N-cadherin (5.6 μm/h). These results suggest that N-cadherin is not likely to function predominantly as a substrate that stimulates migration of O-2A progenitors, but may be more important in initiating early oligodendrocyte-axon interactions that promote the process of myelination.
KW - Cell migration
KW - Immunofluorescence
KW - Merosin
KW - Myelination
KW - O-2A progenitor
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UR - http://www.scopus.com/inward/citedby.url?scp=0011055876&partnerID=8YFLogxK
U2 - 10.1016/S0165-3806(96)00124-1
DO - 10.1016/S0165-3806(96)00124-1
M3 - Article
C2 - 8946049
AN - SCOPUS:0011055876
VL - 97
SP - 9
EP - 15
JO - Developmental Brain Research
JF - Developmental Brain Research
SN - 0165-3806
IS - 1
ER -