TY - JOUR
T1 - N-cadherin and integrins
T2 - Two receptor systems that mediate neuronal process outgrowth on astrocyte surfaces
AU - Tomaselli, Kevin J.
AU - Neugebauer, Karla M.
AU - Bixby, John L.
AU - Lilien, Jack
AU - Reichard, Louis F.
N1 - Funding Information:
The authors would like to acknowledge the generous contributions of antibody-producing hybridomas from Drs. A. F. Horwitz (CSAT) and D. I. Gottlieb fJG22 and lA6). The GFAP antiserum was the generous gift of Dr. L. F. Eng. We thank Dr. J. LaVail for use of the computerized digitizing pad, Dr. T. Large for help with the figures, Marion D. Meyerson for typing the manuscript, and Drs. Z. Hall, Y. N. Jan, 5. Lisberger, G. Napolitano, and S. Crittenden for their helpful comments on the manuscript. K. J. T. is a recipient of a predoctoral Chancellor’s Award from the University of California; K. M. N. is a predoctoral fellow of the National Science Foundation. This work was supported by National Institutes of Health grant NS 19090, NSF grant DCB 8511052, March of Dimes Birth Defects Foundation grant l-774, and the Howard Hughes Medical Institute. L. F. R. is an investigator of the Howard Hughes Medical Institute.
PY - 1988/3
Y1 - 1988/3
N2 - Receptor-mediated interactions between neurons and astroglia are likely to play a crucial role in the growth and guidance of CNS axons. Using antibodies to neuronal cell surface proteins, we identified two receptor systems mediating neurite outgrowth on cultured astrocytes. N-cadherin, a Ca2+-dependent cell adhesion molecule, functions prominently in the outgrowth of neurites on astrocytes by E8 and E14 chick ciliary ganglion (CG) neurons. β1-class integrin ECM receptor heterodimers function less prominently in E8 and not at all in E14 neurite outgrowth on astrocytes. The lack of effect of integrin β1 antibodies on E14 neurite out-growth reflects an apparent loss of integrin function, as assayed by E14 neuronal attachment and process out-growth on laminin. N-CAM appeared not to be required for neurite outgrowth by either E8 or E14 neurons. Since N-cadherin and integrin β1 antibodies together virtually eliminated E8 CG neurite outgrowth on cultured astrocytes, these two neuronal receptors are probably important in regulating axon growth on astroglia in vivo.
AB - Receptor-mediated interactions between neurons and astroglia are likely to play a crucial role in the growth and guidance of CNS axons. Using antibodies to neuronal cell surface proteins, we identified two receptor systems mediating neurite outgrowth on cultured astrocytes. N-cadherin, a Ca2+-dependent cell adhesion molecule, functions prominently in the outgrowth of neurites on astrocytes by E8 and E14 chick ciliary ganglion (CG) neurons. β1-class integrin ECM receptor heterodimers function less prominently in E8 and not at all in E14 neurite outgrowth on astrocytes. The lack of effect of integrin β1 antibodies on E14 neurite out-growth reflects an apparent loss of integrin function, as assayed by E14 neuronal attachment and process out-growth on laminin. N-CAM appeared not to be required for neurite outgrowth by either E8 or E14 neurons. Since N-cadherin and integrin β1 antibodies together virtually eliminated E8 CG neurite outgrowth on cultured astrocytes, these two neuronal receptors are probably important in regulating axon growth on astroglia in vivo.
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U2 - 10.1016/0896-6273(88)90207-3
DO - 10.1016/0896-6273(88)90207-3
M3 - Article
C2 - 2856086
AN - SCOPUS:0023967587
VL - 1
SP - 33
EP - 43
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 1
ER -