Myogenic differentiation of human bone marrow mesenchymal stem cells on a 3D nano fibrous scaffold for bladder tissue engineering

Hong Tian, Shantaram Bharadwaj, Yan Liu, Haiyun Ma, Peter X. Ma, Anthony Atala, Yuanyuan Zhang

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Current strategies for engineering bladder tissues include a bladder biopsy for in vitro cell expansion for use in reconstructive procedures. However, this approach cannot be used in patients with bladder cancer who need a complete bladder replacement. Bone marrow mesenchymal stem cells (BMSC) might be an alternative cell source to better meet this need. We investigated the effects of soluble growth factors, bladder extracellular matrix (ECM), and 3D dynamic culture on cell proliferation and differentiation of human BMSC into smooth muscle cells (SMC). Myogenic growth factors (PDGF-BB and TGF-β1) alone, or combined either with bladder ECM or dynamic cultures, induced BMSC to express smooth muscle-specific genes and proteins. Either ECM or the dynamic culture alone promoted cell proliferation but did not induce myogenic differentiation of BMSC. A highly porous poly-l-lactic acid (PLLA) scaffold provided a 3D structure for maximizing the cell-matrix penetration, maintained myogenic differentiation of the induced BMSC, and promoted tissue remolding with rich capillary formation in vivo. Our results demonstrate that myogenic-differentiated BMSC seeded on a nano fibrous PLLA scaffold can be potentially used for cell-based tissue engineering for bladder cancer patients requiring cystoplasty.

Original languageEnglish
Pages (from-to)870-877
Number of pages8
JournalBiomaterials
Volume31
Issue number5
DOIs
StatePublished - Feb 1 2010
Externally publishedYes

Fingerprint

Tissue Engineering
Scaffolds (biology)
Stem cells
Mesenchymal Stromal Cells
Tissue engineering
Bone
Urinary Bladder
Bone Marrow
Cell culture
Extracellular Matrix
Cell proliferation
Lactic acid
Urinary Bladder Neoplasms
Muscle
Lactic Acid
Intercellular Signaling Peptides and Proteins
Cell Proliferation
Biopsy
Scaffolds
Smooth Muscle Myocytes

Keywords

  • Bladder
  • Bone marrow
  • Scaffold
  • Smooth muscle cell
  • Stem cells

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Myogenic differentiation of human bone marrow mesenchymal stem cells on a 3D nano fibrous scaffold for bladder tissue engineering. / Tian, Hong; Bharadwaj, Shantaram; Liu, Yan; Ma, Haiyun; Ma, Peter X.; Atala, Anthony; Zhang, Yuanyuan.

In: Biomaterials, Vol. 31, No. 5, 01.02.2010, p. 870-877.

Research output: Contribution to journalArticle

Tian, Hong ; Bharadwaj, Shantaram ; Liu, Yan ; Ma, Haiyun ; Ma, Peter X. ; Atala, Anthony ; Zhang, Yuanyuan. / Myogenic differentiation of human bone marrow mesenchymal stem cells on a 3D nano fibrous scaffold for bladder tissue engineering. In: Biomaterials. 2010 ; Vol. 31, No. 5. pp. 870-877.
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