Myofibril-Inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl hearts

Chi Zhang, Pingping Jia, Xupei Huang, Gian Franco Sferrazza, Gagani Athauda, Mohan P. Achary, Jikui Wang, Sharon L. Lemanski, Dipak K. Dube, Larry F. Lemanski

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The Mexican axolotl, Ambystoma mexicanum, carries the naturally-occurring recessive mutant gene 'c' that results in a failure of homozygous (c/c) embryos to form hearts that beat because of an absence of organized myofibrils. Our previous studies have shown that a noncoding RNA, Myofibril-Inducing RNA (MIR), is capable of promoting myofibrillogenesis and heart beating in the mutant (c/c) axolotls. The present study demonstrates that the MIR gene is essential for tropomyosin (TM) expression in axolotl hearts during development. Gene expression studies show that mRNA expression of various tropomyosin isoforms in untreated mutant hearts and in normal hearts knocked down with double-stranded MIR (dsMIR) are similar to untreated normal. However, at the protein level, selected tropomyosin isoforms are significantly reduced in mutant and dsMIR treated normal hearts. These results suggest that MIR is involved in controlling the translation or post-translation of various TM isoforms and subsequently of regulating cardiac contractility.

Original languageEnglish
Article number81
JournalJournal of Biomedical Science
Volume16
Issue number1
DOIs
StatePublished - Oct 5 2009
Externally publishedYes

Fingerprint

Ambystoma mexicanum
Tropomyosin
Muscle Development
Myofibrils
RNA
Protein Isoforms
Genes
Recessive Genes
Untranslated RNA
Essential Genes
Gene expression
Embryonic Structures
Gene Expression
Messenger RNA

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Cell Biology
  • Biochemistry, medical
  • Endocrinology, Diabetes and Metabolism
  • Pharmacology (medical)

Cite this

Zhang, C., Jia, P., Huang, X., Sferrazza, G. F., Athauda, G., Achary, M. P., ... Lemanski, L. F. (2009). Myofibril-Inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl hearts. Journal of Biomedical Science, 16(1), [81]. https://doi.org/10.1186/1423-0127-16-81

Myofibril-Inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl hearts. / Zhang, Chi; Jia, Pingping; Huang, Xupei; Sferrazza, Gian Franco; Athauda, Gagani; Achary, Mohan P.; Wang, Jikui; Lemanski, Sharon L.; Dube, Dipak K.; Lemanski, Larry F.

In: Journal of Biomedical Science, Vol. 16, No. 1, 81, 05.10.2009.

Research output: Contribution to journalArticle

Zhang, C, Jia, P, Huang, X, Sferrazza, GF, Athauda, G, Achary, MP, Wang, J, Lemanski, SL, Dube, DK & Lemanski, LF 2009, 'Myofibril-Inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl hearts', Journal of Biomedical Science, vol. 16, no. 1, 81. https://doi.org/10.1186/1423-0127-16-81
Zhang, Chi ; Jia, Pingping ; Huang, Xupei ; Sferrazza, Gian Franco ; Athauda, Gagani ; Achary, Mohan P. ; Wang, Jikui ; Lemanski, Sharon L. ; Dube, Dipak K. ; Lemanski, Larry F. / Myofibril-Inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl hearts. In: Journal of Biomedical Science. 2009 ; Vol. 16, No. 1.
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