Myocardial mononuclear cell infiltrates are not associated with increased serum cardiac troponin i in cynomolgus monkeys

Michael E. Dunn, Denise Coluccio, Tanja S. Zabka, Gopakumar Gopalakrishnan, Gerard Hirkaler, Wanping Geng, Rosemary Nicklaus, Steven E Lipshultz, Lucette Doessegger, Brett H. Saladino, Thomas Singer, Igor Mikaelian

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Myocardial mononuclear cell infiltrate is a spontaneous cardiac finding commonly identified in laboratory cynomolgus monkeys. The infiltrates are predominantly composed of macrophages with lesser lymphocytes and are not typically associated with histologically detectable cardiomyocyte degeneration. These infiltrates are of concern because they confound interpretation of test article-related histopathology findings in nonclinical safety toxicology studies. The interpretation of safety studies would be simplified by a biomarker that could identify myocardial infiltrates prior to animal placement on study. We hypothesized that monkeys with myocardial mononuclear cell infiltrates could be identified before necropsy using an ultrasensitive immunoassay for cardiac troponin I (cTnI). Serum cTnI concentrations in monkeys with myocardial infiltrates were not higher than those in monkeys without infiltrates at any of the sampling times before and on the day of necropsy. Increased serum cTnI levels are not suitable for screening monkeys with myocardial mononuclear cell infiltrates before placement in the study.

Original languageEnglish
Pages (from-to)647-650
Number of pages4
JournalToxicologic Pathology
Volume40
Issue number4
DOIs
StatePublished - Jun 1 2012

Fingerprint

Troponin
Troponin I
Macaca fascicularis
Haplorhini
Serum
Lymphocytes
Macrophages
Biomarkers
Safety
Screening
Animals
Immunoassay
Cardiac Myocytes
Toxicology
Sampling

Keywords

  • biomarker
  • cardiac troponin I
  • heart
  • infiltrate
  • monkey
  • myocardial inflammation
  • spontaneous background disease
  • ultrasensitive assay

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Cell Biology
  • Molecular Biology

Cite this

Dunn, M. E., Coluccio, D., Zabka, T. S., Gopalakrishnan, G., Hirkaler, G., Geng, W., ... Mikaelian, I. (2012). Myocardial mononuclear cell infiltrates are not associated with increased serum cardiac troponin i in cynomolgus monkeys. Toxicologic Pathology, 40(4), 647-650. https://doi.org/10.1177/0192623311436176

Myocardial mononuclear cell infiltrates are not associated with increased serum cardiac troponin i in cynomolgus monkeys. / Dunn, Michael E.; Coluccio, Denise; Zabka, Tanja S.; Gopalakrishnan, Gopakumar; Hirkaler, Gerard; Geng, Wanping; Nicklaus, Rosemary; Lipshultz, Steven E; Doessegger, Lucette; Saladino, Brett H.; Singer, Thomas; Mikaelian, Igor.

In: Toxicologic Pathology, Vol. 40, No. 4, 01.06.2012, p. 647-650.

Research output: Contribution to journalArticle

Dunn, ME, Coluccio, D, Zabka, TS, Gopalakrishnan, G, Hirkaler, G, Geng, W, Nicklaus, R, Lipshultz, SE, Doessegger, L, Saladino, BH, Singer, T & Mikaelian, I 2012, 'Myocardial mononuclear cell infiltrates are not associated with increased serum cardiac troponin i in cynomolgus monkeys', Toxicologic Pathology, vol. 40, no. 4, pp. 647-650. https://doi.org/10.1177/0192623311436176
Dunn, Michael E. ; Coluccio, Denise ; Zabka, Tanja S. ; Gopalakrishnan, Gopakumar ; Hirkaler, Gerard ; Geng, Wanping ; Nicklaus, Rosemary ; Lipshultz, Steven E ; Doessegger, Lucette ; Saladino, Brett H. ; Singer, Thomas ; Mikaelian, Igor. / Myocardial mononuclear cell infiltrates are not associated with increased serum cardiac troponin i in cynomolgus monkeys. In: Toxicologic Pathology. 2012 ; Vol. 40, No. 4. pp. 647-650.
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