Myocardial infarction in the guinea pig: Cellular electrophysiology

Marion S. Gaide, John S. Cameron, Cynthia B. Altman, Robert J Myerburg, Arthur L. Bassett

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The cellular electrophysiology of left ventricular preparations from guinea pig hearts was studied 1 hour, 24 hours, and 4-6 weeks after myocardial infarction produced by 6-8 single ties of the distal left coronary artery system or after sham operation. Microelectrode recordings were used to monitor cells from the endocardial surface of each preparation in tissue bath. All coronary ligated preparations displayed accelerated spontaneous activity compared to normal and sham operated preparations. Single and multiple premature ventricular depolarizations occurred frequently in coronary ligated and rarely in normal and sham operated preparations. Premature stimuli delivered to areas overlying and bordering the area of infarction, induced short bursts of self-terminating rapid repetitive ventricular activity in 4 of 8 (50%) acute (1-hour), 5 of 9 (55%) subacute (24-hour), and 14 of 20 (70%) healed (4-6-week) infarcted preparations. Such activity could not be induced in normal and sham operated preparations. The preparations with healed infarction were unique in that they demonstrated runs of self-terminating repetitive ventricular activity which occured spontaneously or was inducible with premature stimulation. Recordings from multiple sites in acute, subacute, and healed preparations revealed a variety of transmembrane action potential abnormalities (i.e., reduced action potential amplitude and resting potential, decreased and increased action potential duration, and depressed maximum rates of phase 0 depolarization) in cells overlying and bordering areas of infarction. Only Purkinje fiber action potentials were recorded over the healed infarcts. These data demonstrate that a spectrum of electrophysiological alterations occur in response to ischemic injury and persist after healing of the injury in this new model of myocardial infarction utilizing the guinea pig.

Original languageEnglish
Pages (from-to)2391-2401
Number of pages11
JournalLife Sciences
Volume36
Issue number25
DOIs
StatePublished - Jun 24 1985
Externally publishedYes

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Electrophysiology
Depolarization
Action Potentials
Guinea Pigs
Myocardial Infarction
Infarction
Microelectrodes
Membrane Potentials
Tissue
Purkinje Fibers
Fibers
Wounds and Injuries
Baths
Coronary Vessels

ASJC Scopus subject areas

  • Pharmacology

Cite this

Myocardial infarction in the guinea pig : Cellular electrophysiology. / Gaide, Marion S.; Cameron, John S.; Altman, Cynthia B.; Myerburg, Robert J; Bassett, Arthur L.

In: Life Sciences, Vol. 36, No. 25, 24.06.1985, p. 2391-2401.

Research output: Contribution to journalArticle

Gaide, MS, Cameron, JS, Altman, CB, Myerburg, RJ & Bassett, AL 1985, 'Myocardial infarction in the guinea pig: Cellular electrophysiology', Life Sciences, vol. 36, no. 25, pp. 2391-2401. https://doi.org/10.1016/0024-3205(85)90343-1
Gaide, Marion S. ; Cameron, John S. ; Altman, Cynthia B. ; Myerburg, Robert J ; Bassett, Arthur L. / Myocardial infarction in the guinea pig : Cellular electrophysiology. In: Life Sciences. 1985 ; Vol. 36, No. 25. pp. 2391-2401.
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