Myocardial infarction and intramyocardial injection models in swine

Frederic C. McCall, Kartik S. Telukuntla, Vasileios Karantalis, Viky Y. Suncion, Alan W. Heldman, Muzammil Mushtaq, Adam R. Williams, Joshua M. Hare

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

Sustainable and reproducible large animal models that closely replicate the clinical sequelae of myocardial infarction (MI) are important for the translation of basic science research into bedside medicine. Swine are well accepted by the scientific community for cardiovascular research, and they represent an established animal model for preclinical trials for US Food and Drug Administration (FDA) approval of novel therapies. Here we present a protocol for using porcine models of MI created with a closed-chest coronary artery occlusion-reperfusion technique. This creates a model of MI encompassing the anteroapical, lateral and septal walls of the left ventricle. This model infarction can be easily adapted to suit individual study design and enables the investigation of a variety of possible interventions. This model is therefore a useful tool for translational research into the pathophysiology of ventricular remodeling and is an ideal testing platform for novel biological approaches targeting regenerative medicine. This model can be created in approximately 8-10 h.

Original languageEnglish (US)
Pages (from-to)1479-1496
Number of pages18
JournalNature protocols
Volume7
Issue number8
DOIs
StatePublished - Aug 1 2012

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    McCall, F. C., Telukuntla, K. S., Karantalis, V., Suncion, V. Y., Heldman, A. W., Mushtaq, M., Williams, A. R., & Hare, J. M. (2012). Myocardial infarction and intramyocardial injection models in swine. Nature protocols, 7(8), 1479-1496. https://doi.org/10.1038/nprot.2012.075