Myocardial glycolytic and gluconeogenic enzyme activity during cardiopulmonary bypass in humans

S. F G Wren, Tomas Salerno, M. Crowe, A. J. De Bold

Research output: Contribution to journalArticle

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Abstract

Through a study of the activity of the regulatory enzyme of glycolysis phosphofructokinase and the regulatory enzyme of gluconeogenesis fructose-1,6-diphosphate, myocardial metabolism has been evaluated in the human heart during cardiopulmonary bypass. Three ventricular transmural biopsy specimens were obtained at open-heart surgery before cardiopulmonary bypass was instituted and three similar specimens were taken 30 minutes after cardiopulmonary bypass was started. The enzyme assays were performed on the supernatant obtained from myocardial cells disrupted by sonication. The activity of both enzymes increased during the anoxic conditions of cardiopulmonary arrest. The increase in phosphofructokinase activity is likely due to an overall increase in glycolytic activity, but the presence and increased activity of fructose-1,6-diphosphatase was unexpected.

Original languageEnglish
Pages (from-to)604-605
Number of pages2
JournalCanadian Journal of Surgery
Volume24
Issue number6
StatePublished - Dec 1 1981
Externally publishedYes

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Cardiopulmonary Bypass
Phosphofructokinases
Enzymes
Fructose-Bisphosphatase
Sonication
Gluconeogenesis
Enzyme Assays
Glycolysis
Heart Arrest
Thoracic Surgery
Biopsy

ASJC Scopus subject areas

  • Surgery

Cite this

Myocardial glycolytic and gluconeogenic enzyme activity during cardiopulmonary bypass in humans. / Wren, S. F G; Salerno, Tomas; Crowe, M.; De Bold, A. J.

In: Canadian Journal of Surgery, Vol. 24, No. 6, 01.12.1981, p. 604-605.

Research output: Contribution to journalArticle

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