Myocardial changes during the progression of left ventricular pressure-overload by renal hypertension or aortic constriction: Myosin, myosin ATPase and collage

Patricia L. Kozlovskis, L. A. Fieber, D. K. Pruitt, B. K. Bailey, M. J.D. Smets, A. L. Bassett, S. Kimura, R. J. Myerburg

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

We measured the interrelationships between ventricular muscle myosin mass, myosin ATPase activity and collagen in cats with varying degrees of hypertrophy from left ventricular (LV) pressure-overload produced by either aortic banding or renal hypertension. In order to compare two models of LV pressure-overload with different time courses of progression, the results were analyzed as a function of LV mass or LV weight/body weight (LV/BW) ratio. Myosin was quantitated by SDS-polyacrylamide gel electrophoresis and hydroxyproline was measured as an index of collagen. Myosin concentration was positively correlated with increasing LV mass in control cats. However, in pressure-overloaded LV, myosin concentration was elevated and nearly constant for LV<9.0 g, but decreased in LV>9.0 g. Myosin concentration in pressure-overloaded LV was greatest before a significant increase in LV/BW ratio. Hydroxyproline concentration was inversely related to myosin concentration in both LV pressure-overload models and increased with the severity of hypertrophy. Actomyosin ATPase activity in pressure-overloaded LV, was not significantly different from control over a wide range of LV/BW ratios. However, absolute myosin ATP hydrolysis in pressure-overloaded LV, increased by as much as 40%, relative to control, due primarily to increased myosin. The changing spectrum and interrelationships of myosin and collagen were independent of the mechanism of pressure-overload, but were correlated with the severity of hypertrophy.

Original languageEnglish (US)
Pages (from-to)105-114
Number of pages10
JournalJournal of Molecular and Cellular Cardiology
Volume19
Issue number1
DOIs
StatePublished - Jan 1987

Keywords

  • Collagen
  • Left ventricular pressure-overload
  • Myocardial hypertrophy
  • Myosin
  • Myosin ATPase

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

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